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Application of blackberry seed polysaccharide in aspect of preparing antithrombotic drugs

A technology of blackberry seeds and polysaccharides, applied in the field of medicine and/or health care products, can solve the problems of necrosis, tissue ischemia and the like, and achieve good antithrombotic effect.

Inactive Publication Date: 2016-05-04
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Once blocked, tissue ischemia and necrosis will occur, resulting in serious consequences

Method used

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  • Application of blackberry seed polysaccharide in aspect of preparing antithrombotic drugs
  • Application of blackberry seed polysaccharide in aspect of preparing antithrombotic drugs
  • Application of blackberry seed polysaccharide in aspect of preparing antithrombotic drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1 Extraction, separation and purification of blackberry seed polysaccharide

[0030] 1.1 Extraction and separation of blackberry seed polysaccharide

[0031] Crush blackberry seeds, degrease 3 times with petroleum ether at room temperature, evaporate petroleum ether to dryness, extract 3 times with 70V% ethanol, filter, dry the residue in the shade, then add distilled water at a ratio of 20mL / g to liquid, at 80°C Extract 3 times, 3h each time. Suction filtration while it is hot, combine the filtrates and concentrate under reduced pressure, adjust the final concentration of ethanol to 70V% with 95V% ethanol, let it stand at 4°C for 24h, then centrifuge (10000r / min, 10min), the obtained precipitate is dissolved in distilled water and dialyzed (48h , change distilled water every 4 hours) to remove small molecule impurities, concentrate under reduced pressure, and freeze-dry to obtain blackberry seed crude polysaccharide.

[0032] Dissolve 300 mg of blackberry seed cr...

Embodiment 2

[0051] This example is an antithrombotic test of the blackberry seed polysaccharides (BSP-1, BSP-2, BSP-3) obtained in Example 1.

[0052] Methods: The acute blood stasis model in rats was established by subcutaneous injection of epinephrine hydrochloride and ice-water bath. Rats were given 7 consecutive gavages to investigate the effects of blackberry seed polysaccharide on the four items of blood rheology and coagulation in acute blood stasis model rats.

[0053] Instruments and materials: HF6000-4 semi-automatic coagulation analyzer, HT-100C blood rheological viscosity tester, TGL-16gR high-speed desktop refrigerated centrifuge (Shanghai Anting Scientific Instrument Factory); prothrombin time (PT) determination kit ( 105262), activated partial thrombin time (APTT) assay kit (1121781), thrombin time (TT) assay kit (121119), fibrinogen (FIB) content assay kit (132079) are all provided by Shanghai Sun Biotechnology Ltd. production.

[0054] Experimental animals: SPF grade SD...

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Abstract

The invention relates to application of blackberry seed polysaccharide in the aspect of preparing antithrombotic drugs. The blackberry seed polysaccharide is obtained through the steps of obtaining crude polysaccharide by smashing blackberry seeds and using a water extraction and alcohol precipitation method, and then obtaining three polysaccharides respectively through DEAE-52 cellulose column chromatography and SephadexG-100 gel column chromatography. The in-vivo antithrombotic effect of the three polysaccharides is studied, and a result shows that the three polysaccharides have a good antithrombotic effect and can be separately used or combined to prepare the antithrombotic drugs.

Description

technical field [0001] The invention belongs to the technical field of medicine and / or health care products, and in particular relates to a method for extracting active components of blackberry seeds and its new application, specifically the application of blackberry seed polysaccharides in the preparation of antithrombotic drugs. Background technique [0002] Blackberry Rubusspp. Blackberry is an aggregate fruit plant of the Rosaceae Rubus genus, not used for medicine, and is one of the four emerging small fruit trees in the world. Native to North America, its sweet and sour fruit has high nutritional value and medicinal value. In addition to fresh food, most of it is made into quick-frozen fruit, fruit juice, fruit wine and jam. Literature search found that the research on blackberry mainly focused on the fruit volatile oil, the fatty acid composition of blackberry seed oil and the content analysis of flavonoids, anthocyanins and vitamin E in blackberry leaves and seeds. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K36/73A61K31/715A61P7/02
CPCC08B37/0003A61K31/715A61K36/73
Inventor 康文艺王金梅连朋丽谢平耀王学标
Owner HENAN UNIVERSITY
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