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Prepn. process for freezing dry powder of bromelain

A technology of bromelain and dry powder, which is applied in freeze-dried transportation, peptide/protein components, and medical preparations containing active ingredients, etc. It can solve problems such as low enzyme activity rate, environmental pollution, and low yield of enzyme activity. Achieve the effect of improving immunity and enhancing drug absorption

Inactive Publication Date: 2008-02-13
王美岭 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are three production processes of bromelain: kaolin adsorption method, tannin precipitation method and organic solvent extraction method. The production process and operation of kaolin adsorption method are more complicated, consume more raw materials, require more equipment, and the total recovery of enzyme activity low yield; the tannin precipitation method has a low yield of enzyme activity, which pollutes the environment; the organic solvent method needs to invest a large amount of organic solvent, the activity rate of the enzyme is not high, and the cost is high
These three methods are all produced with pineapple, which cannot produce highly active bromelain

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] After washing the pineapple stems with water, squeeze the juice and filter, add 2% zinc oxide to the filtrate for adsorption, the pH value is 5, the time is 30 minutes, and the adsorption temperature is 20°C. After adsorption, elution was performed with citric acid buffer, the elution time was 20 minutes, the elution pH value was 5, and the concentration of the eluent was 0.2M. After elution, carry out ultrafiltration concentration, using ceramic ultrafiltration membrane, ultrafiltration membrane cut-off relative molecular weight is 30000, operating temperature is 30 ℃, operating pressure is 0.06Mpa. The ultrafiltration concentrated solution is freeze-dried. First freeze the bromelain solution in an ultra-low temperature refrigerator at -40°C, and then put it in a freeze dryer for freeze-drying. After 2 hours, the temperature rises to -10°C, keeps the temperature for 6 hours, and then gradually increases the temperature to 30°C, the drying is finished, and bromelain fre...

Embodiment 2

[0018] After washing the pineapple stems with water, squeeze the juice and filter, add 3% zinc oxide to the filtrate for adsorption, the pH value is 6, the time is 20 minutes, and the adsorption temperature is 15°C. After the adsorption, the citric acid buffer was used for elution, the elution time was 30 minutes, the elution pH value was 6, and the concentration of the eluent was 0.3M. After desorption, carry out ultrafiltration concentration, using ceramic ultrafiltration membrane, ultrafiltration membrane cut-off relative molecular weight is 25000, operating temperature is 28 ℃, operating pressure is 0.07Mpa. The ultrafiltration concentrated solution is freeze-dried. First freeze the bromelain solution in an ultra-low temperature refrigerator at -40°C, and then put it in a freeze dryer for freeze-drying. After 2 hours, the temperature rises to -10°C, keeps the temperature for 6 hours, and then gradually increases the temperature to 30°C, the drying was completed, and the br...

Embodiment 3

[0020] After washing the pineapple stems with water, squeeze the juice and filter, add 1% zinc oxide to the filtrate for adsorption, the pH value is 4, the time is 40 minutes, and the adsorption temperature is 25°C. After adsorption, elution was carried out with citric acid buffer, the elution time was 40 minutes, the elution pH value was 4.5, and the concentration of the eluent was 0.4M. After desorption, carry out ultrafiltration concentration, using ceramic ultrafiltration membrane, ultrafiltration membrane cut-off relative molecular weight is 35000, operating temperature is 35 ℃, operating pressure is 0.05Mpa. The ultrafiltration concentrated solution is freeze-dried. First freeze the bromelain solution in an ultra-low temperature refrigerator at -40°C, and then put it in a freeze dryer for freeze-drying. After 2 hours, the temperature rises to -10°C, keeps the temperature for 6 hours, and then gradually increases the temperature to 30°C, the drying was completed, and brom...

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Abstract

This invention discloses a method for manufacturing pineapple proteinase freeze-dried powders, which can solve the problem of low activity faced by conventional pineapple proteinase. The method comprises the steps of: washing pineapple stems with water, pressing to obtain juice, filtering, adsorbing the filtrate with zinc oxide, desorbing, ultrafiltering, concentrating and freeze-drying to obtain pineapple proteinase freeze-dried powders, whose enzyme activity can reach 600 million units per gram.

Description

technical field [0001] The invention relates to the technical field of bromelain extraction. Background technique [0002] Bromelain is a kind of protease extracted from pineapple, pineapple peel, and pineapple stem. It is mainly used as a raw material for medicine. It has the functions of anti-inflammatory, improving immunity and enhancing drug absorption. It can treat cardiovascular and cerebrovascular diseases, tumors and inflammation. It can also be used in the production of cheese, gelatin, and hydrolyzed protein in the biochemical industry; as a food additive in the food industry, it can tenderize meat and clarify beer. [0003] At present, there are three production processes of bromelain: kaolin adsorption method, tannin precipitation method and organic solvent extraction method. The production process and operation of kaolin adsorption method are more complicated, consume more raw materials, require more equipment, and the total recovery of enzyme activity The yiel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/50A61K38/48A61K9/19
Inventor 王美岭吴茂玉
Owner 王美岭
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