Colloidal gold test strip for detecting hereditary hearing loss, and preparation method and application thereof
A technology for hereditary deafness and colloidal gold test paper, which is applied in the field of colloidal gold to achieve the effects of accurate results, simple operation and low cost
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[0026] The preparation method of the above-mentioned colloidal gold test paper card for detecting hereditary deafness comprises:
[0027] (1) preparation of colloidal gold solution: in HauCl Add trisodium citrate, after boiling, use trisodium citrate, tannic acid as reducing agent to obtain the colloidal gold solution that diameter is 10-20nm;
[0028] (2) Preparation of colloidal gold binding pad: adjust the colloidal gold solution to pH 7.5-8.5 and add SLC26A4 antibody, GJB2 antibody and blocking agent. After labeling, redissolve the precipitate into the colloidal gold solution containing BSA and sucrose. The reconstituted product is added to glass fiber membrane or polyester membrane and then dried;
[0029] (3) Chromatography pad preparation: SLC26A4 antibody and GJB2 antibody were diluted with PBS, respectively streaked on the nitrocellulose membrane and dried;
[0030] (4) Test paper card assembly: Paste the sample pad, colloidal gold binding pad, chromatographic membra...
Embodiment 1
[0034] This embodiment provides a colloidal gold test card for detecting hereditary deafness, and provides a preparation method of the test card.
[0035] 1. Main materials
[0036] SLC26A4, GJB2 specific paired antibody, goat anti-mouse IgG antibody: Beijing Shengqi Yanghuan Technology Co., Ltd.; chloroauric acid: Sigma company; nitrocellulose (NC) membrane: SARTORIUS (Germany), CN140; Germany Sartorius company Products; bovine serum albumin (BSA), polyethylene glycol PEG20000, hydrolyzed casein: Sigma products. Other commonly used reagents are analytical reagents.
[0037] Two, the preparation method of colloidal gold
[0038] 1. Add HauCl 4 Prepare 0.01% aqueous solution first, take 100ml and heat to boiling.
[0039] 2. Accurately add 1% trisodium citrate (Na 3 C 6 h 5 o 7 2H 2 O) 2.00-4.50ml of aqueous solution.
[0040] 3. Continue heating and boiling for 30 minutes. At this time, it can be observed that the light yellow chloroauric acid aqueous solution turns...
Embodiment 2
[0058] This embodiment provides a colloidal gold kit for detecting hereditary deafness.
[0059] The test kit includes a test paper, a colorimetric card or a colloidal gold quantitative reader, and a plastic card for accommodating the test paper. The test paper is loaded into the plastic card to form a test paper card. Specifically, the test kit also includes a dropper Tubes, Serum Cups and Disposable Syringes. The detection test paper is the colloidal gold test paper card provided in Example 1 for detecting hereditary deafness. The colloidal gold quantitative reader is a known optical detection system.
[0060] The samples applicable to this kit are the same as those applicable to the colloidal gold test paper card for detecting hereditary deafness. Serum, whole blood, urine, etc. can be used as detection samples. In addition, tissue sections, cell smears and other samples for histochemical qualitative tests Can be used as samples.
[0061] The above kit was used to detect...
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