Seaweed azotobacter fertilizer preparation method
A nitrogen-fixing bacteria fertilizer and nitrogen-fixing bacteria technology are applied in the directions of organic fertilizers, inorganic fertilizers, calcareous fertilizers, etc., to achieve the effects of simple process operation, improved application effect and low production cost.
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Embodiment 1
[0014] The invention discloses a preparation method of seaweed nitrogen-fixing bacteria fertilizer, the specific steps are as follows:
[0015] Rinse 1kg of fresh commercially available kelp repeatedly with tap water, dry it and cut it into 1cm-sized pieces; take another 4g of flour, 0.1g of magnesium sulfate, and 2g of calcium carbonate dissolved in a small amount of tap water and mix evenly to make a nutritional supplement; Fully mix the kelp fragments and nutritional supplements to make a solid fermentation substrate, cook at 100°C for 15 minutes, and cool.
[0016] Preparation of nitrogen-fixing bacteria seed liquid: the first-level slant culture adopts Ashubei culture medium, which consists of 0.2 g of potassium dihydrogen phosphate, 0.2 g of magnesium sulfate, 0.2 g of sodium chloride, 5 g of calcium carbonate, 10 g of glucose, and 0.1 g of calcium sulfate. g. 18 g of agar, 1 L of water, pH 7.0, cultured at 28° C. for 48 hours, inoculated with second-grade asbestos liqui...
Embodiment 2
[0020] Experiment purpose and method: In order to evaluate the effectiveness and feasibility of the present invention, this example takes the seaweed nitrogen-fixing bacteria fertilizer prepared in Example 1 as the experimental object, and studies the influence on the growth and development of the wheat variety Tiemai No. 1, and sets up a blank group , common nitrogen-fixing bacteria group and seaweed nitrogen-fixing bacteria group, the blank group was not fertilized, the common nitrogen-fixing bacteria group was given common nitrogen-fixing bacteria fertilizer, and the seaweed nitrogen-fixing bacteria group was given seaweed nitrogen-fixing bacteria fertilizer. The specific operation method is as follows:
[0021] Using Tiemai No. 1 wheat variety, soak the wheat seeds in clean water for 24 hours, and use 0.1% mg / LHgCl 2 The solution was sterilized for 5 minutes, and then rinsed with distilled water for 3 times. Wheat seeds of uniform size were selected to be sown in the steril...
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