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Ganoderma spore wall breaking method

A technology of Ganoderma lucidum spores and Ganoderma lucidum spore powder, which can be used in pharmaceutical formulations, plant raw materials, plant/algae/fungus/moss components, etc. It can solve the problems of loss of active ingredients of Ganoderma lucidum spores, low wall breaking rate, etc., to reduce the difficulty of dissolution, The effect of improving the wall breaking rate

Inactive Publication Date: 2019-02-26
SICHUAN XUYANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a method for breaking the wall of ganoderma lucidum spores, which can effectively solve the problems of low wall breaking rate and serious loss of active ingredients in the ganoderma spores in the existing wall breaking methods

Method used

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Examples

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Embodiment 1

[0029] A method for breaking ganoderma spores, comprising the following steps:

[0030] (1) Pulverization: pulverize the ganoderma spores, and cross a 60-80 mesh sieve to obtain the ganoderma spore powder;

[0031] (2) Oscillation: mix Ganoderma lucidum spore powder, distilled water, and lysozyme in a weight ratio of 1:2:0.01, and oscillate at 25°C for 2 hours to obtain an oscillating mixture;

[0032] (3) Enzymolysis: Mix the oscillating mixed solution and the mixed enzyme solution at a mass ratio of 1:0.3 and then perform enzymolysis. The enzymolysis temperature is 40°C and the enzymolysis time is 4 hours to obtain an enzymolysis solution; the mixed enzyme solution Including cellulase, hemicellulase and pectinase, the concentrations of cellulase, hemicellulase and pectinase are 0.5g / L, 0.3g / L, 0.4g / L respectively;

[0033] (4) Ultrasonic treatment: filter the enzymolysis solution, remove the filtrate, mix the obtained solid with the cellulase solution at a weight ratio of 1...

Embodiment 2

[0039] A method for breaking ganoderma spores, comprising the following steps:

[0040] (1) Pulverization: pulverize the ganoderma spores, and cross a 60-80 mesh sieve to obtain the ganoderma spore powder;

[0041] (2) Oscillation: mix Ganoderma lucidum spore powder, distilled water, and lysozyme in a weight ratio of 1:4:0.02, and oscillate at 35°C for 1 hour to obtain an oscillating mixture;

[0042] (3) Enzymolysis: Mix the oscillating mixed solution and the mixed enzyme solution at a mass ratio of 1:0.8 and then perform enzymolysis. The enzymolysis temperature is 50°C and the enzymolysis time is 3 hours to obtain an enzymolysis solution; the mixed enzyme solution Including cellulase, hemicellulase and pectinase, the concentrations of cellulase, hemicellulase and pectinase are 0.8g / L, 0.6g / L, 0.7g / L respectively;

[0043] (4) Ultrasonic treatment: filter the enzymolysis solution, remove the filtrate, mix the obtained solid with the cellulase solution at a weight ratio of 1:...

Embodiment 3

[0049] A method for breaking ganoderma spores, comprising the following steps:

[0050] (1) Pulverization: pulverize the ganoderma spores, and cross a 60-80 mesh sieve to obtain the ganoderma spore powder;

[0051](2) Oscillation: mix Ganoderma lucidum spore powder, distilled water, and lysozyme in a weight ratio of 1:3:0.15, and oscillate at 30°C for 2 hours to obtain an oscillating mixture;

[0052] (3) Enzymolysis: Mix the oscillating mixed solution and the mixed enzyme solution at a mass ratio of 1:0.5 and then perform enzymolysis. The enzymolysis temperature is 45°C and the enzymolysis time is 4 hours to obtain an enzymolysis solution; the mixed enzyme solution Including cellulase, hemicellulase and pectinase, the concentrations of cellulase, hemicellulase and pectinase are 0.6g / L, 0.5g / L, 0.6g / L respectively;

[0053] (4) Ultrasonic treatment: filter the enzymatic solution, remove the filtrate, mix the obtained solid with the cellulase solution at a weight ratio of 1:6,...

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Abstract

The invention discloses a ganoderma spore wall breaking method. The method comprises crushing, oscillating, enzymolysis, ultrasonic treatment, drying, superfine grinding, freeze drying and ultrasonictreatment processes. The method has the advantages that the enzymolysis, ultrasonic treatment, superfine grinding and freeze drying processes have huge effect on wall breaking, and the oscillating process, within which wall breaking enzyme is added, further has huge effect on the preliminary decomposition of cell wall, so that the later processes exert the biggest effect, the wall breaking efficiency can be improved only through combination of all the steps, the wall breaking rate reaches 99.8 percent, and meanwhile loss of effective components is reduced.

Description

technical field [0001] The invention belongs to the technical field of processing ganoderma spores, and in particular relates to a method for breaking ganoderma spores. Background technique [0002] Existing wall-breaking methods of Ganoderma lucidum spores include biological enzymatic hydrolysis, chemical methods, physical methods, etc., but due to the poor stability of the active ingredients of Ganoderma lucidum spore powder, the existing wall-breaking technology is to ensure that the active ingredients of Ganoderma lucidum spore powder are not stable to the greatest extent. Destroyed, but there is a low wall-breaking rate, and it is difficult to completely break the wall, and the active ingredients in the ganoderma spores are seriously lost. Contents of the invention [0003] Aiming at the above-mentioned shortcomings in the prior art, the present invention provides a method for breaking the wall of ganoderma lucidum spores, which can effectively solve the problems of l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/074
CPCA61K36/074A61K2236/15A61K2236/17A61K2236/19
Inventor 韩业详杨文生
Owner SICHUAN XUYANG PHARMA
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