A kind of tissue culture method for inducing the bulblets of Fritillaria japonicus

Abstract

The present invention discloses a thunberg fritillary bulb bulblet inducement tissue culture method comprising the steps of cutting core buds of thunberg fritillary bulb to be used as explants, sterilization, seedling cultivation, induction culture, expansion cultivation and the like. For the first, the core buds of thunberg fritillary bulb are used as the explants for producing bulblets by induction after seedling by cultivation, the period for producing the bulblets of the thunberg fritillary bulb can be greatly shortened, the whole cultivation process is free of cell dedifferentiation and redifferentiation, the chance of genetic mutation is greatly reduced, and good quality can be guaranteed. The thunberg fritillary bulb bulblet inducement tissue culture method has rapid propagation speed, short culture period, and significant economic benefits popularization and application value.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for tissue culture and propagation of Fritillaria fritillaria, and is a new method for inducing and cultivating bulblets of Fritillaria fritillata. Background technique [0002] Fritillaria thunbergii (Fritillaria thunbergii) is one of the bulk of traditional Chinese medicinal materials commonly used in clinical practice. It is listed as the first of the "Eight Flavors of Zhejiang". At present, in the production of Fritillaria fritillaria, there are mainly 4 cultivars: narrow-leaf species, broad-leaf species, multi-seed species and small three sons. Among them, the narrow-leaf variety Fritillaria fritillaria has the most extensive planting area, and was identified as a new variety in 2007, named Zhebei No. 1; the wide-leaved variety Fritillaria fritillaria was identified as Zhebei No. 2 in 2014. Since the seeds of Fritillaria fritillaria are harvested, it takes 5 years for ...

AI Technical Summary

Problems solved by technology

At present, there are mainly two ways of in vitro rapid propagation of Fritillaria fritillaria: 1. Indirect organ generation: Callus induction by explants of Fritillaria fritillaria, and then induction of regenerated shoots is the most studied rapid propagation method at present, but Since this method needs to undergo cell dedifferentiation and redifferentiation to obtain tissue culture seedlings, there are a large number of genetic variations in this way, which is not conducive to the inheritance of the excellent quality of Fritillaria fritillaria.

2. The direct way of organ generation: this way does not go through the callus tissue, and the explants of Fritillaria fritillaria can directly induce adventitious buds or small bulbs, which can avoid the genetic variation caused by cell differentiation and redifferentiation, and is good for maintaining the germplasm of Fritillaria fritillaria. The traits have positive effects, but there is a lack of research on the rapid propagation of Fritillaria fritillaria through this method, and there are many deficiencies