Slide preparation method for discriminating chromosome number of Avena magna, Triticum aestivum or filial generation of Avena magna and Triticum aestivum
A chromosome number and tetraploid technology, applied in the biological field, can solve the problems of hypotonicity after lack, chromosome sticking together, poor chromosome dispersion, etc., and achieve the effect of good chromosome dispersion, more cell division phases, and high repeatability
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[0046]Example 1 Chromosome Number Analysis of 22 Strains of Tetraploid Large Oat and Common Wheat Hybrid Progeny
[0047] 1. Material preparation
[0048] Soak the seeds of 22 strains of tetraploid large oats and common wheat hybrid progenies obtained in water, place them on moist filter paper and cultivate them at 25°C for germination. When the roots grow to about 10mm, cut out the root tip meristem , quickly follow the steps below.
[0049] 2. Production
[0050] (1) Pretreatment: 0.075mol / L KCl solution, saturated α-bromonaphthalene solution, and distilled water are prepared as a pretreatment solution at a volume ratio of 2:3:5, and the cut root tips are soaked in the pretreatment solution, and treated at 25°C After 3 hours, wash with water for 15 minutes;
[0051] (2) Fixation: Methanol and glacial acetic acid were prepared into a fixative solution at a volume ratio of 3:1, and the pretreated root tips were fixed with the fixative solution, fixed at 25°C for 1 hour, and...
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