Cultivation and domestication method of wild collybia albuminosa
A technology for the cultivation and domestication of wild gallicariae fungus and the original seed culture medium can solve the problems such as the increase of the cultivation cost and the occurrence of ant damage, and achieve the effects of fast fruiting and stable output.
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Embodiment 1
[0017] A method for cultivating and acclimating wild colpodium fruticosa, comprising the following steps:
[0018] S1, separation of mother species: directly extract tender somatic cells from the growing vegetative body of Gallinarum gallinarum to induce callus, gradually cultivate into mycelium, and select pure white, strong mycelium as the mother species;
[0019] S2, the configuration of mother seed culture medium: 50%PDA+50%DDS, described PDA is potato 200g, glucose 20g, agar 20g, water 1000ml;
[0020] S3, inoculating the mycelium with strong growth after the propagation of the mother species into the culture medium of the mother species, cultivating it in a constant temperature incubator at 25°C until the culture medium is covered with mycelia, and selecting the strong mycelia as the original species;
[0021] S4. The configuration of the original seed medium: 25% of sawdust, 45% of DDGS, 20% of bran, 10% of termite discharge and 120% of water.
[0022] S5. Inoculate th...
Embodiment 2
[0027] A method for cultivating and acclimating wild colpodium fruticosa, comprising the following steps:
[0028] S1, separation of mother species: directly extract tender somatic cells from the growing vegetative body of Gallinarum gallinarum to induce callus, gradually cultivate into mycelium, and select pure white, strong mycelium as the mother species;
[0029] S2, the configuration of mother seed culture medium: 50% improved PDA+50% DDS, described improved PDA is potato 200g, glucose 20g, agar 20g, water 1000ml, potassium hydrogen phosphate 1g, magnesium sulfate 0.5g, yeast extract 2g.
[0030] S4, the configuration of the original seed medium: wood chips 25%, DDGS 45%, bran 20%, termite discharge 10% and water 120%;
[0031] S5. Inoculate the original seed in a cultivation bottle filled with the original seed medium, and cultivate it in a constant temperature incubator at 25° C. to obtain the cultivar;
[0032] S6, the configuration of cultivar culture medium: the comp...
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