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Convenient procalcitonin detection kit

A technology for detecting kits and procalcitonin, applied in the field of biomedicine, can solve the problems of easy copying and tampering, false positives, complicated operation process, etc., and achieve the effect of avoiding copying and tampering and improving the simplicity of operation

Inactive Publication Date: 2016-04-06
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection process needs to read the ID chip data and time the reaction twice, the operation process is complicated, and the ID chip has no encryption function, so it is easy to be copied and tampered with; moreover, this method is also susceptible to the interference of rheumatoid factor and heterophile antibody, resulting in false positives

Method used

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  • Convenient procalcitonin detection kit
  • Convenient procalcitonin detection kit
  • Convenient procalcitonin detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Preparation of procalcitonin detection kit

[0049] 1) Preparation of fluorescent tracer-labeled antibody

[0050] Dilute the carboxyl fluorescent microspheres with 10mmol / LpH=6.5 phosphate buffer solution to a concentration of 5mg / ml, and prepare a suspension of microspheres. Add 5mg of EDAC to each 10ml of microsphere suspension, mix well, and stand at room temperature for 1 hour , centrifuge at 10000rpm for 20min, remove the supernatant, and suspend the pellet in an equal volume of 10mmol / LpH=7.2 phosphate buffer, ultrasonically disperse; then add 3mg of procalcitonin monoclonal antibody 1 ( The primary antibody to procalcitonin is the fusion of splenocytes obtained from mice immunized with procalcitonin antigen and bone marrow cells, and the hybridoma cells that can permanently produce the first epitope antibody of procalcitonin are screened and hybridized The mouse monoclonal antibody that can specifically bind to the first epitope of procalcitonin was ...

Embodiment 2

[0068] Example 2: Performance Evaluation of Procalcitonin Detection Kit

[0069] 1) Comparative experiment

[0070] The kit of the present invention and the specific protein meter of beckmanIMMAGE800 detect 176 samples of procalcitonin (whole blood / plasma) in the concentration range of 0.1-50ng / ml at the same time, and the correlation coefficient R2=0.997.

[0071] 2) Interfering experiments

[0072] After adding different amounts of interfering substances to the same human serum sample, the determination is carried out. The difference between the measured value after adding the interfering substance and the measured value before adding the interfering substance divided by the ratio of the measured value before adding the interfering substance is the interference rate. The test results show that the concentrations of human rheumatism factor and human anti-mouse antibody are respectively When they are below 640 μmol / L, 640 μmol / L, 8g / L, 10mmol / L and 400IU / mL, their interferen...

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Abstract

A convenient procalcitonin detection kit comprises a test strip, an upper clamping groove, a lower clamping groove and an RFID (radio frequency identification) chip, wherein the test strip comprises a plastic rubber slab; a sample pad, a first antibody bearing pad, a cellulose membrane and a water absorption pad are arranged sequentially on the plastic rubber slab; the first antibody bearing pad and the water absorption pad are in lap joint at two ends of the cellulose membrane; one end of the sample pad is in lap joint on the first antibody bearing pad; a detection band T for immobilizing a second C-reactive protein antibody and a quality control band C for immobilizing a goat-anti-mouse antibody are arranged on the cellulose membrane; the test strip is arranged in a cavity formed by splicing the upper clamping groove and the lower clamping groove; a sampling hole and a detection window are formed in the upper clamping groove; a sample diluent adopts a buffer containing rheumatoid factors and a heterophil antibody blocker; the RFID chip is arranged in the lower clamping groove close to the left end. The detection kit has the advantages that the antibody activity can be reflected accurately, an error detection result can be avoided, the kit is simple to use, and the like.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a convenient detection kit for procalcitonin. Background technique [0002] At present, quantitative immunoassay technology mainly includes the following quantitative detection methods: [0003] One is the immunoturbidimetric detection technology supporting large-scale, highly integrated automated instruments; [0004] The second is enzyme-linked immunochromatography detection technology; [0005] The third is the rapid detection technology of immunochromatography; [0006] Among them, immunoturbidimetric detection technology and enzyme-linked immunoassay technology have disadvantages such as sample pretreatment, complicated operation, long detection time, complex large-scale equipment, poor mobility, and long reporting time, which are more suitable for centralized detection of large batches of samples. , Not suitable for rapid detection in emergency department and outpati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/68G01N33/533G01N1/38
CPCG01N33/558G01N1/38G01N33/533G01N33/6803
Inventor 邹炳德邹继华方亮刘献文
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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