Application of 2-acetamido-1,5,-dehydrated-2-deoxy-D-glucitol to preparation of anti-rice-leaf-blight-bacterial-activity medicine

A technology of rice bacterial blight and acetylamino, which is applied in the field of agricultural microbiology to achieve the effect of new selectivity and strong antagonistic ability

Inactive Publication Date: 2016-04-06
HUAZHONG AGRI UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no report on the use of 2-acetylamino-1,5,-anhydro-2-deoxy-D-glucitol (2-Acetamido-1,5-anhydro-2-deoxy-D-glucitol, also known as 1-deoxy-N-acetylglucosamine) for biological control work, as an aminoglycoside analog with specific antibacterial activity, 2-Acetamido-1,5-anhydro-2-deoxy-D-glucitol is likely to become A new type of agricultural antibiotic

Method used

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  • Application of 2-acetamido-1,5,-dehydrated-2-deoxy-D-glucitol to preparation of anti-rice-leaf-blight-bacterial-activity medicine
  • Application of 2-acetamido-1,5,-dehydrated-2-deoxy-D-glucitol to preparation of anti-rice-leaf-blight-bacterial-activity medicine
  • Application of 2-acetamido-1,5,-dehydrated-2-deoxy-D-glucitol to preparation of anti-rice-leaf-blight-bacterial-activity medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Determination of Minimum Inhibitory Concentration of 2-Acetamido-1,5-anhydro-2-deoxy-D-glucitol against Xanthomonas oryzae

[0020] Principle: According to the law of diffusion, the logarithm of the total amount of antibiotics has a linear relationship with the square of the antibacterial diameter. When the antibiotic concentration reaches or exceeds the MIC value, the test bacteria are inhibited and cannot reproduce, thus presenting a transparent zone of inhibition.

[0021] LogY=kX 2 +b (Y is the concentration, X is the diameter of the inhibition zone)

[0022] 1) Xanthomonas oryzae pv. oryzae PXO99A is activated by streaking to obtain a single colony. A single colony is picked and inoculated with PSA medium at 28° C. and cultured at 180 rpm to the logarithmic growth phase.

[0023] 2) Prepare 2-Acetamido-1,5-anhydro-2-deoxy-D-glucitol with a concentration of 25, 50, 100, 200 μg / mL, and use water as a negative control.

[0024] 3) Add 1% pathogenic bacteria to the melted solid...

experiment example 2

[0026] The tube-dish method determines the activity of 2-Acetamido-1,5-anhydro-2-deoxy-D-glucitol against common pathogenic bacteria:

[0027] 1) The pathogenic bacteria Escherichia coli, Staphylococcus aureus and Salmonella are activated, and a single colony is selected to inoculate LB medium at 37°C and cultured at 180 rpm to the logarithmic growth phase. The pathogenic bacteria Pseudomonas solanacearum and Pseudomonas syringae were activated, and a single colony was picked to inoculate LB medium at 28°C and cultured at 180 rpm to the logarithmic growth phase.

[0028] 2) Prepare 2-Acetamido-1,5-anhydro-2-deoxy-D-glucitol with a concentration of 100μg / mL, and use water as a negative control.

[0029] 3) Add 1% pathogenic bacteria to the melted solid medium (add it when the flask is not hot), place an Oxford cup in the poured plate and add 100ul of the test solution, 3 repeats, E. coli, Staphylococcus aureus and Salmonella They were cultured at 37°C, and Pseudomonas solanacearum an...

experiment example 3

[0034] Determination of 2-Acetamido-1,5-anhydro-2-deoxy-D-glucitol activity against common pathogenic fungi by punching method

[0035] 1) Pathogenic fungi Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia solani, Aspergillus niger, Botrytis cinerea, Gibberella wheat, Botrytis cinerea, Penicillium italia, Sclerotium canola, Banana Fusarium oxysporum was activated, and the hyphae were picked to inoculate PDA medium and cultured at 28°C.

[0036] 2) Prepare 2-Acetamido-1,5-anhydro-2-deoxy-D-glucitol with a concentration of 100μg / mL, and use water as a negative control.

[0037] 3) Pick the bacterial clumps in the center of the PDA solid medium plate, punch 6 holes at equal distances with a puncher, add 20 μl of the test solution, 3 replicates, and culture at 28°C. The observation results are shown in Table 2.

[0038] Table 2: 2-Acetamido-1,5-anhydro-2-deoxy-D-glucitol antifungal spectrum

[0039]

[0040]

[0041] +: means there is effect, -: means no effect.

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Abstract

The invention discloses application of 2-acetamido-1,5,-dehydrated-2-deoxy-D-glucitol to preparation of an anti-rice-leaf-blight-bacterial-activity medicine. It is found for the first time that 2-acetamido-1,5,-dehydrated-2-deoxy-D-glucitol has anti-rice-leaf-blight-bacterial-activity. After 2-acetamido-1,5,-dehydrated-2-deoxy-D-glucitol is prepared into antibacterial medicine, it is found that rice leaf blight bacterial antimicrobial activity of 2-acetamido-1,5,-dehydrated-2-deoxy-D-glucitol is specific, antagonistic ability is strong, the minimal inhibitory concentration of 2-acetamido-1,5,-dehydrated-2-deoxy-D-glucitol is only 23.90 ug / ml, and 2-acetamido-1,5,-dehydrated-2-deoxy-D-glucitol has no inhibition activity on various common pathogenic bacteria and pathogenic fungi. Therefore, the technical scheme provides a new choice for preparation of a novel preparation preventing rice leaf blight.

Description

Technical field [0001] The invention belongs to the technical field of agricultural microbiology, and specifically relates to the application of 2-acetylamino-1,5,-anhydro-2-deoxy-D-glucitol in the preparation of active medicines against bacterial leaf blight of rice. Background technique [0002] Rice bacterial blight disease is caused by the bacterial disease Xanthomonasoryzaepv.oryzae. The disease was first discovered in Fukuoka, Japan in 1884, and the disease area continued to expand with the planting and transportation. Since the 1950s of the last world, the scope of the disease has expanded, and the incidence has spread to rice producing areas around the world, and the incidence is more serious in China, Japan and India. The area of ​​rice bacterial blight disease that occurs perennially in my country is more than 10 million mu, and the yield is generally reduced by 10%-20%. In severe cases, the yield is reduced by 50%-80%, or even no harvest. [0003] The control methods of...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01P3/00
CPCA01N43/16
Inventor 张吉斌邵宗泽郑龙玉李光玉蔡珉敏孙风芹喻子牛黄典张少博陈莉
Owner HUAZHONG AGRI UNIV
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