Method for producing saccharide solution

A manufacturing method, technology of sugar solution, applied to methods using microorganisms, production of sugar, boiling/evaporation of sugar solution, etc., can solve problems of cost, remove object limitation, and invest a lot of energy

Active Publication Date: 2016-03-30
TORAY IND INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the hydrolysis of cellulose-containing biomass, there is a problem that sugars such as glucose and xylose are decomposed simultaneously with the decomposition of cellulose or hemicellulose components, and furfural, hydroxymethyl, etc. are also produced. Furan compounds such as furfural, or by-products such as organic acids such as formic acid, acetic acid, and levulinic acid
However, in such a method of evaporative removal, there is a problem that the removal efficiency of fermentation inhibitors such as organic acids with low boiling points is low depending on the boiling point of the fermentation inhibitors, and a large amount of energy must be invested in order to o

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  • Method for producing saccharide solution
  • Method for producing saccharide solution
  • Method for producing saccharide solution

Examples

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Embodiment

[0108] Examples are given below to describe the present invention in detail. However, the present invention is not limited to these.

reference example 1

[0109] (Reference Example 1) Measurement of Sugar Concentration

[0110] The concentrations of glucose and xylose contained in the sugar solution were quantified by comparison with standard products under the HPLC conditions shown below.

[0111] Column: LunaNH 2 (manufactured by Phenomenex)

[0112] Mobile phase: Milli-Q:acetonitrile=25:75 (flow rate 0.6mL / min)

[0113] Reaction solution: no

[0114] Detection method: RI (differential refractive index)

[0115] Temperature: 30°C.

reference example 2

[0116] (Reference Example 2) Analysis of Fermentation Inhibiting Substances

[0117] Aromatic compounds and furan compounds contained in the hydrolyzate were quantified by comparison with standard samples under the HPLC conditions shown below. In addition, each analysis sample was centrifuged at 3500G for 10 minutes, and the supernatant was used for the analysis described below.

[0118] Column: Synergi Hidro RP4.6mm×250mm (manufactured by Phenomenex)

[0119] Mobile phase: Acetonitrile-0.1%H 3 PO 4 (flow rate 1.0mL / min)

[0120] Detection method: UV (283nm)

[0121] Temperature: 40°C.

[0122] Acetic acid and formic acid were quantified by comparison with standard products under the HPLC conditions shown below. In addition, each analysis sample was subjected to centrifugation at 3500G for 10 minutes, and the supernatant fraction was used for the analysis described below.

[0123] Column: Shim-Pack and Shim-Pack SCR101H (manufactured by Shimadzu Corporation) in series ...

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Abstract

[Problem] An aqueous saccharide solution obtained from biomass contains various fermentation-inhibiting substances, however, it is possible to easily remove fermentation-inhibiting substances derived from biomass at a low cost by treating the aqueous saccharide solution with microbes that do not utilize glucose or xylose or with a crude enzyme product derived from said microbes.

Description

technical field [0001] The present invention relates to a method for producing sugar liquor from biomass. Background technique [0002] Fermentative production processes of sugar-based chemicals are used in the production of various industrial raw materials. As the sugar used as the raw material for this fermentation, sugar derived from edible raw materials such as sugarcane, starch, and sugar beets is currently used industrially, but from the ethical perspective of the skyrocketing price of edible raw materials caused by the increase in the world population in the future, or competition with food The process of efficiently producing sugar liquid from recycled non-edible resources, that is, cellulose-containing biomass, or the process of efficiently converting the obtained sugar liquid into industrial raw materials is a future issue. [0003] As a method for producing sugar liquid from cellulose-containing biomass, a method of producing sugar liquid by acid hydrolysis of ce...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P7/06C12P19/02
CPCC12P19/02C12P19/14C12N1/22C12P7/10C12P2203/00Y02E50/10C12P7/14C13B20/002C13B30/002C13B50/00C13K1/04C13K5/00C13K13/00C13K13/002
Inventor 栗原宏征石冢裕子山田胜成
Owner TORAY IND INC
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