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Method for synthesizing selenium and bismuth selenide nano material by using lysinibacillus sp.

A technology of Bacillus lysinus and nanomaterials, applied in the field of environmental nanobiology, can solve the problems of no biological synthesis of bismuth selenide and no biosynthesis of bismuth selenide, and achieve easy expansion and cultivation, simple operation, and environmental protection. green friendly effect

Active Publication Date: 2016-03-30
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, currently reported Bi 2 Se 3 The synthesis methods are all chemical or physical methods, and there is no report on the biological synthesis of bismuth selenide.
[0005] Retrieval of literature and patent results on the synthesis of bismuth selenide at home and abroad shows that before the application of the present invention, there has been no report on the biosynthesis of bismuth selenide under aerobic conditions

Method used

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  • Method for synthesizing selenium and bismuth selenide nano material by using lysinibacillus sp.
  • Method for synthesizing selenium and bismuth selenide nano material by using lysinibacillus sp.
  • Method for synthesizing selenium and bismuth selenide nano material by using lysinibacillus sp.

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Example 1: Under plate coating culture conditions, strain ZYM-1 reduces selenite to synthesize selenium nanoparticles

[0027] Reaction system: After diluting the ZYM-1 bacterial solution in 20ml of LB liquid medium containing 0.5-25mM (mmol) sodium selenite for 8-12 hours to different times, spread it evenly on the surface containing 1 mM sodium selenite on LB solid medium. Incubate for 36 h in a constant temperature incubator at 30°C. The color of the colony changed from light yellow to red, revealing the generation of the reduction product selenium nanoparticles, and the photos of the cultivated plates were as follows: figure 1 shown.

Embodiment 2

[0028] Example 2: The reduction degree of bacterial strain ZYM-1 to selenite under different sodium selenite concentrations

[0029] Add 0.5, 1, 2.5, 5, 10, and 25 mM sodium selenite to 20 mL of liquid LB medium respectively, and the inoculum amount of ZYM-1 bacteria solution is 1%, and the culture temperature is 30 ° C, pH is 5.5, 100 rpm Cultivate for 48 hours; the composition of the LB liquid medium is: sodium chloride 10g / L, peptone 10g / L, yeast powder 5g / L. The generated elemental selenium nanoparticles were treated with Na 2 S oxidation, calculation of selenium content, the specific method is as follows:

[0030] 1. Prepare 1M Na 2 S solution, add an appropriate amount of NaOH solid to suppress Na 2Autohydrolysis of S. Prepare 1M NaCl solution. Add different amounts of commercial selenium powder into 10ml test tubes, add 5mLNa 2 The S solution was fully shaken and dissolved so that the concentrations of selenium in the standard solution were 0.5, 1, 2, 3, 4, 5 mM r...

Embodiment 3

[0032] Example 3: Effects of different pH on reduction of selenite by bacterial strains.

[0033] Use HCl or NaOH to adjust the pH of the liquid LB medium to 4, 5, 6, 7, 8, 9, 10, 11, respectively, and ensure that the total volume of the culture system is 20 mL. The inoculation amount of the bacterial liquid ZYM-1 was 1%, the concentration of selenite was 5mM, the culture temperature was 30°C, and 200rpm was cultivated for 36h; the composition of the LB liquid medium was: sodium chloride 10g / L, peptone 10g / L, yeast Powder 5g / L. Utilize Na 2 The elemental selenium nanoparticles generated by S oxidation, the specific measurement method is as follows:

[0034] The sample containing bacteria and intracellular selenium nanoparticles was centrifuged at 10000rpm for 10min and separated from the supernatant. Rinse 2 times with 1M NaCl solution to remove residual selenite in the solution, and then wash the residual solid with 5mL of 1M NaCl 2 S was fully shaken to dissolve, and the...

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Abstract

The invention relates to a method for synthesizing selenium and a bismuth selenide nano material by using lysinibacillus sp.ZYM-1. The method comprises the following steps: adding 0.5-25mM of sodium selenite into an LB culture medium subjected to high-temperature sterilization at 121 DEG C, inoculating ZYM-1, and culturing for 24-48h under the condition that the temperature is 25-40 DEG C, the pH value is 5-9 and the speed is 50-300rpm to obtain a red electroselenium nano material; and adding 0.5-25mM of sodium selenite and 0.1-5mM of bismuth nitrate solution into the LB culture medium subjected to high-temperature sterilization at 121 DEG C, inoculating ZYM-1, and culturing for 24-48h under the condition that the temperature is 25-40 DEG C, the pH value is 5-9 and the speed is 50-300rpm to obtain a black bismuth selenide nano material. The reaction is carried out in aqueous solution without severe conditions of high temperature, high pressure and the like, so that cultivation can be enlarged easily. The prepared selenium nano spheres can be applied to adsorption removal of heavy metals, and biosynthesis of the prepared bismuth selenide nano material is not reported at present.

Description

technical field [0001] The invention belongs to the field of environmental nano-biology technology, and relates to a method for synthesizing selenium and bismuth selenide nanomaterials by using lysine bacillus. Background technique [0002] Selenium belongs to the sixth main group element and is an essential trace element for biological survival. However, high concentrations of selenium are biologically toxic and cause environmental pollution. Selenium in nature mainly exists in four valence states (-2, 0, +4 and +6). Among them, selenite (SeO 3 2- ) is easily soluble in water and has stronger biological toxicity than other forms. The researchers found that a variety of microorganisms that widely exist in nature can reduce selenite through detoxification to produce red elemental selenium nanoparticles with less toxicity and poor water solvent. In the literature (BMCMicrobiology.2014.14.204-217) by WangGJ et al., LampisS et al. in the literature (Microbialcellfactories.20...

Claims

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Application Information

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IPC IPC(8): C12P3/00C12R1/01
Inventor 周豪赵永和胡玉峰董宇轩栗李萌潘海霞柳丽芬
Owner DALIAN UNIV OF TECH
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