Method for synthesizing high-fluorescent alloy nano cluster by taking small molecules as template

A photoalloy and small molecule technology, applied in the field of bioengineering, can solve the problems of low fluorescence intensity and poor stability, and achieve the effects of enhancing fluorescence intensity, improving stability, and easy-to-control synthesis methods.

Inactive Publication Date: 2016-03-30
DALIAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, people's research on nanoclusters has gradually shifted from expensive gold, silver, and platinum to cheap copper, but the synthesized copper nanoclusters have great shortcomings, such as poor stability and low fluorescence intensity.

Method used

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  • Method for synthesizing high-fluorescent alloy nano cluster by taking small molecules as template
  • Method for synthesizing high-fluorescent alloy nano cluster by taking small molecules as template
  • Method for synthesizing high-fluorescent alloy nano cluster by taking small molecules as template

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Synthesis of copper nanoclusters

[0030] Add 5mL of copper nitrate solutions of different concentrations to 5mL of 80mM reduced glutathione solution, so that the molar ratios of reduced glutathione to copper ions are 8:1, 6:1, 4:1, 2: 1. Adjust the pH to 5 with 1M NaOH solution and mix well at room temperature;

[0031] Fluorescent detection at an excitation wavelength of 350 nm by figure 1 Therefore, the molar ratio of glutathione to copper nitrate is 4:1, which is better than other ratios. When glutathione is excessive, due to the interaction between glutathione and copper nitrate, a complex is generated to cause fluorescence of quenching.

Embodiment 2

[0033] Synthesis of alloy copper-silver nanoclusters

[0034] ①Add 5mL of 20mM copper nitrate solution to 5mL of 80mM reduced glutathione solution, adjust the pH to 5 with 1M NaOH solution, and mix well at room temperature;

[0035] ②Add different concentrations of 1mL silver nitrate solution to the product obtained in step ①, so that the molar ratios of copper ions and silver ions are 5:2, 5:3, 5:4, 1:1, 5:6, and mixed uniform;

[0036] Fluorescent detection under UV light, by figure 2 Therefore, when the molar ratio of copper ions to silver ions is 5:4, the fluorescence intensity is the highest. Adding silver nitrate solution not only increases the fluorescence intensity of copper nanoclusters, but also improves their stability. image 3 is the fluorescence spectrum of copper nanoclusters and alloy copper-silver nanoclusters. Figure 4 1 and 2 are the luminescence diagrams of copper nanoclusters and alloy copper-silver nanoclusters under visible light respectively, the ...

Embodiment 3

[0038] Synthesis of alloy copper-silver nanoclusters

[0039] ① Mix 5mL 80mM reduced glutathione solution with 5mL 20mM copper nitrate solution, adjust the pH to 5 with 1M NaOH solution, and mix well at room temperature;

[0040] ② Add 1mL 80mM silver nitrate solution to the product obtained in step ① and mix well;

[0041] ③ Add 10%, 20%, 40%, and 60% ethanol to the product obtained in step ② and mix evenly;

[0042] The product obtained in step ③ was diluted 2 times by volume respectively, and the fluorescence detection was performed by Figure 5 Therefore, ethanol induces the phenomenon of aggregation in the system, thereby further increasing the fluorescence intensity of the system.

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Abstract

The invention relates to a method for synthesizing a high-fluorescent alloy nano cluster by taking small molecules as a template, and belongs to the technical field of bioengineering. The method comprises the following steps: (1) mixing glutathione solution with copper ion solution uniformly, and regulating the pH to 4-5, wherein the mole rate of glutathione to copper ions is (4-8):1; and (2) adding silver ions into a product obtained in the step (1), and mixing uniformly, wherein the mole rate of glutathione to copper ions to silver ions is (20-40):5:(4-6). The method provided by the invention has the beneficial effects that by introducing the silver ions to synthesize an alloy copper-silver nano cluster, the fluorescence intensity of a copper nano cluster is enhanced, the stability of the cluster is improved, and a new nano cluster is provided. Moreover, the synthetic method provided by the invention is easy to control, and is simple and quick.

Description

technical field [0001] The invention relates to a method for synthesizing high-fluorescence alloy nanoclusters by using small molecules as templates, and belongs to the technical field of bioengineering. Background technique [0002] Metal nanoclusters are composed of a few to a dozen atoms. Because of their low toxicity, small size, strong photostability, and good water solubility, metal nanoclusters have gradually become a widely used method in many fields of nanotechnology. nanomaterials. [0003] The synthesis of metal nanoclusters mainly adopts the template synthesis method. The commonly used templates include polymers, DNA, polypeptides and proteins. Recently, the use of small biological molecules to synthesize metal nanoclusters has attracted widespread attention. This is because the synthesis of metal nanoclusters by biological small molecules has its superior conditions, including mild reaction conditions, good biocompatibility, simple and clear structure, and smal...

Claims

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Application Information

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IPC IPC(8): C09K11/58B22F9/24B22F1/00B82Y40/00
CPCC09K11/58B22F9/24B22F1/054
Inventor 韩冰雁侯绪芬相荣超许杰
Owner DALIAN UNIV OF TECH
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