Device for improving peak capacity of capillary electrophoresis through capillary electrophoresis two-step speed difference mode

Abstract

The invention discloses a device for improving the peak capacity of capillary electrophoresis through a capillary electrophoresis two-step speed difference mode. The device comprises a negative pole liquid storage tank (1), a positive pole liquid storage tank (2), a sample tank (4) and a capillary tube (5) and further comprises a first zero-pole liquid storage tank (3) and a second zero-pole liquid storage tank (13); the capillary tube (5) is arranged to penetrate through the first zero-pole liquid storage tank (3) and the second zero-pole liquid storage tank (13), one end of the capillary tube (5) is movably connected with the positive pole liquid storage tank (2) or the sample tank (4), the other end of the capillary tube (5) is movably connected with the negative pole liquid storage tank (1) or the sample tank (4), and a gap which can conduct electricity and cannot conduct mass transfer is formed in the portion, in each zero-pole liquid storage tank, of the capillary tube. By means of the device for improving the peak capacity of the capillary electrophoresis, the requirement for the self separation capacity of the capillary tube is reduced, even though partial components in a sample cannot reach the baseline separation, the baseline separation can be finally achieved by means of the method, and therefore the whole peak capacity of the capillary electrophoresis is improved. In addition, a low content component signal is not lost when the peak capacity is improved through the device.

Description

technical field [0001] The invention relates to a device for improving capillary electrophoresis peak capacity in two-step speed difference mode of capillary electrophoresis. Background technique [0002] Peak capacity is the maximum number of peaks that can be separated to a certain degree of resolution within a certain period of time under a given separation system and operating conditions. Peak capacity has become an effective measure of the separation ability of a separation method. In chromatographic separation, methods to increase peak capacity mainly include two-dimensional or multidimensional chromatographic separation, gradient elution, increasing the length of the separation column, and changing the particle size of the stationary phase. [0003] But for capillary electrophoresis, there is no mature method to accomplish this goal. Generally, the peak capacity can only be increased by improving the resolution, that is, increasing the effective separation length of ...

AI Technical Summary

Problems solved by technology

However, excessively increasing the capillary length reduces the resolution due to sample diffusion; optimization of the background electrolyte conditions can improve the resolution to a limited extent; reducing the sample injection volume can improve the resolution between components, but for some complex samples (such as biological samples) ), the content of various components varies greatly, and reducing the injection volume also means the loss of low-content samples, resulting in the absence of detection of these components; High-voltage electric field divides the sample zone into several zones according to the swimming speed of each component. Since the amount of sample in each zone is reduced, the resolution is increased.

This method improves the resolution of capillary electrophoresis to a large extent, but it is only suitable for the case where the component peaks in the sample do not overlap seriously, otherwise it will not achieve good results

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