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Dual gradient system in liquid chromatography and matched elution program setting method thereof

A liquid chromatography and gradient technology, which is applied in the field of instrument analysis, can solve the problems of being unable to be eluted and replaced, and the effective bonding sites gradually decrease, and achieve the requirements of low hardware requirements, wide range of strength changes, and improved service life. Effect

Active Publication Date: 2021-07-13
SHIMADZU (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One of the reasons for the decline in column efficiency after a long period of use is that these ultra-low polar compounds are firmly combined with the bonded phase and cannot be eluted and replaced, which means that the effective bonding sites gradually decrease, and the column efficiency will definitely drop.

Method used

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  • Dual gradient system in liquid chromatography and matched elution program setting method thereof
  • Dual gradient system in liquid chromatography and matched elution program setting method thereof
  • Dual gradient system in liquid chromatography and matched elution program setting method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] In the dual gradient system of this embodiment, the liquid phase combination pump uses two pumps, one pump directly pumps the weak solvent water, and the other pump has a built-in gradient proportional valve to pump the strong solvent A acetonitrile and the strong solvent B isopropanol respectively , wherein the elution capacity of isopropanol is much higher than that of acetonitrile; the separation column is a C18 column of Shimadzu.

[0056] As a comparison, in this embodiment, the system is used to separate the same group of targets in normal mode and dual gradient mode. The common mode does not use a double elution gradient, and the weak elution solvent used is water, and the strong solvent is acetonitrile; A super strong elution solvent isopropanol was used.

[0057] The gradient elution program of normal mode without double elution gradient is as follows: image 3 Shown, the total flow velocity of described liquid phase combination pump pumping solvent is 0.4mL / ...

Embodiment 2

[0061] In the dual gradient system of this embodiment, the liquid phase combination pump uses two pumps, one pump directly pumps the weak solvent water, and the other pump has a built-in gradient proportional valve to pump the strong solvent A acetonitrile and the strong solvent B isopropanol respectively , wherein the elution capacity of isopropanol is much higher than that of acetonitrile; the separation column is the T3 column of WATERS.

[0062] As a comparison, in this embodiment, the system is used to separate the same group of targets in normal mode and dual gradient mode. The common mode does not use a double elution gradient, and the weak elution solvent used is water, and the strong solvent is acetonitrile; A super strong elution solvent isopropanol was used.

[0063] The gradient elution program and conditions of the common mode in this embodiment are the same as those of Example 1, and the gradient elution program and conditions of the dual gradient mode of this e...

Embodiment 3

[0066] In the dual gradient system of this embodiment, the liquid phase combination pump uses two pumps, one pump directly pumps the weak solvent water, and the other pump has a built-in gradient proportional valve to pump the strong solvent A acetonitrile and the strong solvent B isopropanol respectively , wherein the elution capacity of isopropanol is much higher than that of acetonitrile; the separation column is a C18 column of Shimadzu.

[0067] As a comparison, in this embodiment, the system is used to separate the same group of targets in normal mode and dual gradient mode. The common mode does not use a double elution gradient, and the weak elution solvent used is water, and the strong solvent is acetonitrile; A super strong elution solvent isopropanol was used.

[0068] The gradient elution program and conditions of the common mode in this embodiment are the same as those of Example 1, and the gradient elution program and conditions of the dual gradient mode of this ...

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Abstract

The invention relates to a dual gradient system in liquid chromatography and a matched elution program setting method thereof. The system comprises a liquid phase combination pump, a mixer, a sample introduction device, a separation column and a detector which are connected in sequence, the liquid phase combination pump can independently pump at least three solvents to the mixer as mobile phases, the three solvents are respectively a weak solvent, a strong solvent A and a strong solvent B, and the elution capacities of the weak solvent, the strong solvent A and the strong solvent B on a separated target object are sequentially enhanced; the liquid phase combination pump forms a first elution gradient and a second elution gradient of a mobile phase by adjusting the ratio of pumped solvents, the first elution gradient is the ratio of the weak solvent to the sum of the strong solvent A and the strong solvent B, and the second elution gradient is the ratio of the strong solvent A to the strong solvent B. According to the system and the elution program, the separation capacity of wide-polarity compounds in the separation column can be improved, and ultra-low-polarity compounds in the separation column can be cleaned out.

Description

technical field [0001] The invention relates to the field of instrument analysis, in particular to a dual gradient system in liquid chromatography and a matching method for setting elution programs. Background technique [0002] Reverse-phase liquid chromatography is suitable for the separation of low and medium polar compounds. During the separation process, the compound is subjected to the dual effects of the binding force of the column bonded phase and the elution force of the mobile phase. During the entire separation process, if the polarity of the compound is relatively moderate, and the eluting force of the mobile relative to it is stronger than its binding force with the bonded phase, the target will be gradually pushed forward by the mobile phase, and finally washed by the mobile phase Out of the chromatographic column and out of the peak. [0003] However, if the compound is of very low polarity (i.e., strongly retentive compound), then its binding to the reverse...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/60
CPCG01N30/02G01N30/06G01N30/60G01N2030/065
Inventor 郑云重钟启升申玲玲邝江濛刘佳琪黄涛宏
Owner SHIMADZU (CHINA) CO LTD
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