Protein interaction network construction method

Abstract

The present invention discloses a protein interaction network construction method. The method comprises the following steps: acquiring a protein set that is interested in; constructing a first-level interaction protein database of a target protein, and drawing a first-level protein interaction network of the target protein according to the first-level interaction protein database; and constructing a second-level interaction protein database of the target protein, and in conjunction with the first-level protein interaction network of the target protein, drawing an interaction protein database of the target protein. Compared with the prior art, according to the method disclosed by the present invention the candidate database used when constructing the protein interaction network is comprehensive and extensive, and the idea of integrated use and expanded application of an existing database is novel; the protein interaction database can comprehensively and extensively store existing protein interaction relationships, the source is reliable, and the query is convenient; and a reliable basis is provided for experimental verification of unknown protein functions.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for constructing a protein interaction network. Background technique [0002] With the continuous supplement of human protein data, more and more unknown functions of proteins need to be discovered for biomedical research. Most proteins must be combined with other proteins or biomacromolecules to perform their functions. Therefore, to study protein functions, it is necessary to clarify the information of the proteins that interact with them. And based on the function of interacting proteins, speculate and verify the role of the target protein in cell function and biological process. At present, there are many high-throughput methods for identifying protein interactions, and they are stored in different protein databases. The existing protein interaction databases are as follows: [0003] 1. HPRD (HumanProteinReferenceDatabase) only collects human protein interaction (PPI)...

AI Technical Summary

Problems solved by technology

[0010] However, due to data redundancy and high false positives among various databases, it is difficult to effectively use the protein interaction information stored in a single protein database.

In addition, due to the different data sources in existing protein databases, the rapid increase of protein and sequence information, and the limitations of experimental methods, the protein interaction information stored in a single protein database lags far behind.