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A Method for Obtaining Hippeastrum 'Apple' Hybrid by Rescuing Immature Embryos in Vitro

A technology for in vitro rescue and hybridization of immature embryos, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of obtaining hybrid germplasm without hybridization of immature embryos, abortion of hybrid embryos, hindrance to hybrid breeding, etc. problem, to achieve the effect of improving the survival rate of hybrids, shortening the cultivation period, and having strong operability

Active Publication Date: 2017-09-29
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, previous studies on cross pollination for many years found that 'Apple' is a female sterile variety, that is, when it is used as a female parent, its hybrid embryos will be aborted and cannot bear fruit (Ye Lu, 2007; Lv Wentao et al., 2010; Shi Fengrui et al., 2014), which has greatly hindered people's research work on cross breeding
Therefore, there is an urgent need for a complete set of artificial embryo rescue technology to obtain the excellent hybrid germplasm resources of 'apple', but before that, there is no method for its hybrid immature embryo rescue and hybrid germplasm, let alone a successful example

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The object of the present invention is to provide a kind of method that obtains Hippeastrum 'apple' hybrid by immature embryo ex vivo rescue, and its steps are as follows:

[0023] 1), the preparation of culture medium:

[0024] (1) Basic medium: MS medium, wherein sucrose 30g / L, agar 7g / L, pH=5.8;

[0025] (2) Embryo rescue medium: MS+6-BA 0.1~0.5mg / L+NAA0.01~0.05mg / L+glutamine 200~600mg / L+hydrolyzed casein 200~800mg / L+0.1~0.4g / L Activated carbon;

[0026] (3) Strong seedling rooting medium: MS+6-BA 0.01~0.03mg / L+NAA0.05~0.3mg / L;

[0027] 2) Cross-pollination and selection of explants: use 'Apple' as the female parent, lightly smear the pollen of the male parent on the cracked stigma of 'Apple' for cross-pollination and bagging, about 2 to 3 weeks after pollination extraction of immature ovaries;

[0028] 3), explant disinfection and inoculation: take the whole immature ovary as the explant material, first soak it with detergent solution for 20 minutes and then cle...

Embodiment 2

[0032] The object of the present invention is to provide a kind of method that obtains Hippeastrum 'apple' hybrid by immature embryo ex vivo rescue, and its steps are as follows:

[0033] 1), the preparation of culture medium:

[0034] (1) Basic medium: MS medium, in which sucrose 20-30g / L, agar 5-9g / L, pH=5.8;

[0035] (2) Embryo rescue medium: MS+6-BA 0.1mg / L+NAA 0.02mg / L+glutamine 400mg / L+hydrolyzed casein 400mg / L+0.2g / L activated carbon;

[0036] (3) Strong seedling rooting medium: MS+6-BA 0.02mg / L+NAA 0.2mg / L;

[0037] 2), cross-pollination and selection of explants: use 'Apple' as the female parent, lightly smear the pollen of the male parent 'Dancing Queen' on the cracked stigma of 'Apple' for cross-pollination and bagging, after pollination The immature ovary is extracted at about 2 to 3 weeks;

[0038] 3), explant disinfection and inoculation: take the whole immature ovary as the explant material, first soak it with detergent solution for 20 minutes and then clean ...

Embodiment 3

[0042] The object of the present invention is to provide a kind of method that obtains Hippeastrum 'apple' hybrid by immature embryo ex vivo rescue, and its steps are as follows:

[0043] 1), the preparation of culture medium:

[0044] (1) Basic medium: MS medium, in which sucrose 20-30g / L, agar 5-9g / L, pH=5.8;

[0045] (2) Embryo rescue medium: MS+6-BA 0.2mg / L+NAA 0.05mg / L+glutamine 200mg / L+hydrolyzed casein 400mg / L+0.2g / L activated carbon;

[0046] (3) Strong seedling rooting medium: MS+6-BA 0.01mg / L+NAA 0.1mg / L;

[0047] 2), cross-pollination and selection of explants: use 'Apple' as the female parent, lightly smear the pollen of the male parent 'Flower Peacock' on the cracked stigma of 'Apple' for cross-pollination and bagging, after pollination The immature ovary is extracted in about 2 to 3 weeks;

[0048] 3), explant disinfection and inoculation: take the whole immature ovary as the explant material, first soak it with detergent solution for 20 minutes and then clean...

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PUM

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Abstract

The invention discloses a method for obtaining a hybrid of Hippeastrum 'apple' by rescuing immature embryos in vitro. The female sterile Hippeastrum 'apple' is used as the female parent for hybrid pollination, and the immature embryos are taken out for embryonic breeding before abortion. The rescue culture enables the hybrid immature embryos to develop into normal seeds, thereby obtaining a variety of 'apple' hybrid offspring. This method effectively improves the survival rate of hybrids and shortens the breeding cycle of 'apple' hybrid offspring, which improves the favorable technical guarantee for the development of its breeding work, and has positive significance for the innovation of germplasm resources of Hippeastrum chinensis.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a method for obtaining a hybrid of Hippeastrum 'apple' through in vitro rescue of immature embryos. Background technique [0002] Hippeastrum Herb. is a perennial bulbous plant of the genus Hippeastrum Herb in the family Amaryllidaceae. Its flowers are large and colorful and bloom in winter and spring. When in full bloom, the flowers and dark green leaves complement each other, and are of high ornamental value. Hippeastrum is not only an ideal potted flower and high-grade cut flower material for the New Year's Eve, but also can beautify the landscape in the open garden flower arrangement. However, wild species of Hippeastrum are mainly distributed in tropical and subtropical regions such as South America and Brazil, and there is no distribution of wild Hippeastrum resources in my country. Therefore, my country's Hippeastrum market is overly dependent on imports, and the v...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H4/00
CPCA01H1/02A01H4/008
Inventor 王燕吕永平牟豪杰汪一婷陈剑平陈志
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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