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Method used for promoting biological reduction of nitrobenzene with biological carbon

A technology of nitrobenzene and biochar, which is applied in the field of biological treatment of pollutants, can solve the problems of high material cost and low treatment efficiency, and achieve the effects of simple preparation process, strong operability and improved biological reduction rate

Inactive Publication Date: 2016-02-24
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method of using biochar to quickly and effectively promote the biological reduction of nitrobenzene, so as to solve the problems of low processing efficiency and high material cost of existing biological treatment technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Biochar obtained from different biomass raw materials and preparation methods is used to promote the reduction of nitrobenzene by microorganisms:

[0018] (A) Preparation of biochar:

[0019] The natural incineration method is to place the wheat and cotton stalks in the outdoor air to fully burn them, and collect the burnt carbonization products. The oxygen-limited pyrolysis method is that the wheat and cotton straw materials are respectively placed in a tubular pyrolysis furnace, heated to 600°C at a heating rate of 5°C / min, pyrolyzed at a constant temperature for 2 hours, and nitrogen is continuously passed during the pyrolysis process. It not only ensures the anaerobic environment in the furnace, but also sweeps the gaseous organic products out of the pyrolysis furnace. After natural cooling, the charred product was taken out. The charred products of wheat and cotton stalks after natural incineration and oxygen-limited pyrolysis were washed 3-5 times with deionized...

Embodiment 2

[0027] Biochar promotes microbial reduction of nitrobenzene at different concentrations:

[0028] (A) Select wheat straw natural burning biochar as the biochar material in this example.

[0029] (B) cultivation of microorganisms: with embodiment 1.

[0030] (C) Prepare 50 mL of 20 mM phosphate buffer solution, add 50 mM sodium lactate, transfer it to a 50 mL glass anaerobic bottle, and adjust the pH to 7.0 with 0.1 mol / L NaOH or HCl. Then aerated with nitrogen to exhaust the oxygen in the headspace of the anaerobic bottle and the aqueous solution, and put it into the anaerobic box to ensure the anaerobic state.

[0031] (D) In ​​the anaerobic box, transfer nitrobenzene to different anaerobic bottles with a pipette gun to obtain 50 mL reaction systems containing 5-100 mg / L nitrobenzene respectively.

[0032] (E) Weigh the wheat incinerated biochar, transfer it to an anaerobic bottle in the anaerobic box, make the biochar concentration 0.5g / L, and ultrasonically disperse it in...

Embodiment 3

[0036] The promotion effect of different biochar material concentrations on microbial reduction of nitrobenzene:

[0037] (A) Select wheat straw natural burning biochar as the biochar material in this example.

[0038] (B) cultivation of microorganisms: with embodiment 1.

[0039] (C) Prepare 50 mL of 20 mM phosphate buffer solution, add 50 mM sodium lactate, transfer it to a 50 mL glass anaerobic bottle, and adjust the pH to 7.0 with 0.1 mol / L NaOH or HCl. Then aerated with nitrogen to exhaust the oxygen in the headspace of the anaerobic bottle and the aqueous solution, and put it into the anaerobic box to ensure the anaerobic state.

[0040] (D) In ​​the anaerobic box, transfer nitrobenzene to an anaerobic bottle with a pipette gun to obtain a 50 mL reaction system containing 100 mg / L nitrobenzene.

[0041] (E) Weigh the biochar separately, transfer it to the anaerobic bottle in the anaerobic box, so that the biochar concentration is 0.167, 0.333, 0.500, 0.667g / L respectiv...

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Abstract

The invention discloses a method used for promoting biological reduction of nitrobenzene with biological carbon, and belongs to the technical field of pollutant biological treatment. The method comprises following steps: biological carbon with a concentration ranging from 0.167 to 0.667g / L is added into 50mL of solution containing 5 to 400mg / L of nitrobenzene; an obtained mixture is delivered into an anaerobic bottle; 0.5g / L of dissimilatory reduction bacteria cells are added; and then the anaerobic bottle is delivered into a constant temperature shaking table for 1 to 10d of dark reaction at 30 DEG C at a speed of 150rpm. The biological carbon used in the method comprises biological carbon prepared via natural burning method and oxygen limited pyrolysis. The biological carbon can be used for increasing biological reduction rate of nitrobenzene in water rapidly and effectively; a preparation method of the biological carbon is simple; raw materials are widely available and cheap; operationality is high; and the method is convenient for popularization and application.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of pollutants, in particular to a method for using biochar to promote the reduction of nitrobenzene by microorganisms. Background technique [0002] As an important intermediate for the synthesis of dyes, explosives, rubber, pesticides and other fine chemicals, nitrobenzene is widely present in industrial wastewater such as dyes, pesticides, medicines, and petrochemicals. Nitrobenzene is a refractory organic compound with stable chemical properties and high toxicity to organisms. After entering the water environment, it will gradually seep into the soil with groundwater, causing persistent pollution of water and soil, thereby causing a series of ecological and environmental effects. [0003] Biological method is a common method for treating nitrobenzene wastewater. Microorganisms such as dissimilatory metal-reducing bacteria can reduce nitrobenzene to aniline under anaerobic conditio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/28C02F3/34C12N1/20C12R1/01
Inventor 柳广飞刘乐成周集体金若菲
Owner DALIAN UNIV OF TECH
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