Method for cultivating agaricus bisporus through liquid strains

Abstract

The invention discloses a method for cultivating agaricus bisporus through liquid strains and belongs to the technical field of mushroom cultivation. Mannitol, tryptone, starch and agar are taken and prepared into a culture medium, after the pH is adjusted, heating sterilization is conducted, and agaricus bisporus spores are inoculated and statically cultivated for use; wort, corn flour, potassium phosphate and other matter are taken and mixed, deionized water is added, standby agaricus bisporus strains are inoculated after high-temperature sterilization, shaker shaking cultivation is conducted after still standing, then the agaricus bisporus strains and distilled water are mixed, bagasse obtained after smashing, wetting fermentation, immersion in added whitewash and sterilization is added for blending, the mixture is placed in a polypropylene plastic bag to be cultivated, and the mixture is cultivated at certain temperature after earthing and watering. The method has the advantages that the cost by the method for cultivating agaricus bisporus is lower than that by other methods by 5%, the production period is short, the contamination rate is low, no massive supply of labor or fields are needed, and management is convenient. The cultivated agaricus bisporus is high in quality, the yield is higher than that by other methods by 7% or above, fungus ages are consistent, mechanical operation is facilitated, and the method is suitable for large-scale production.

Description

technical field [0001] The invention relates to a method for cultivating Agaricus bisporus with liquid strains, and belongs to the technical field of mushroom cultivation. Background technique [0002] Agaricus bisporus is also known as white mushroom, mushroom, and sea mushroom, and producers and operators in European and American countries often call it common cultivated mushroom or button mushroom. Agaricus bisporus is a mushroom that is cultivated and consumed worldwide. It is known as the "World Mushroom" and can be sold fresh, canned, or salted. The mycelium of Agaricus bisporus is also used as a raw material for medicine. China's Agaricus bisporus is most cultivated in Fujian, Shandong, Henan, Zhejiang and other provinces. Cultivation methods include mushroom house cultivation, greenhouse frame cultivation and greenhouse border cultivation. Different regions, different climatic conditions and different seasons can adopt their own cultivation methods. It is widely ...

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention: the production of solid strains is generally multiplied by test tube parent species into secondary and tertiary species, the production cycle is long, the bacterial age is inconsistent, the pollution rate is high, and a large amount of labor and space are required, resulting in Due to the disadvantages of low yield, difficult production management and high cost, a method for cultivating Agaricus bisporus with liquid strains is provided. The present invention is to prepare a medium by taking mannitol, tryptone, starch and agar, and after adjusting the pH, the temperature is raised to extinguish Agaricus bisporus spores were inoculated and cultured for later use; then wort, corn flour, potassium phosphate and other substances were mixed, and deionized water was added, after high-temperature sterilization, inoculated with the spare Agaricus bisporus strains, and shaker shaker after standing The cultured stock solution of Agaricus bisporus young plants is mixed with distilled water, mixed with bagasse that has been crushed, wetted and fermented, soaked in lime water, sterilized, put into a polypropylene plastic bag for cultivation, covered with soil, sprinkled with water, and kept at a certain temperature down to cultivate