Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Sample pretreatment method for simultaneous determination of oxalic acid, succinic acid, sulfuric acid and phosphate radicals in regenerated tobacco leaves through ion chromatography

A technology for sample pretreatment and reconstituted tobacco leaves, which is applied to measurement devices, material separation, instruments, etc., and can solve the problems of incomplete extraction of oxalic acid, failure to consider the influence of vitamin C, and inaccurate measurement results.

Active Publication Date: 2016-02-17
CHINA TOBACCO GUANGDONG IND
View PDF6 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are many ion chromatography methods for the determination of oxalic acid content in plants and foods containing vitamin C (ascorbic acid) including tobacco, but the above literatures do not consider the influence of vitamin C on the determination of oxalic acid, and only consider vitamins in the medical detection of biological fluids. The effect of C on the determination of oxalic acid
There is a key technical problem in the current methods for determining oxalic acid in tobacco: the extraction of oxalic acid with pure water is incomplete, resulting in inaccurate determination results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Sample pretreatment method for simultaneous determination of oxalic acid, succinic acid, sulfuric acid and phosphate radicals in regenerated tobacco leaves through ion chromatography
  • Sample pretreatment method for simultaneous determination of oxalic acid, succinic acid, sulfuric acid and phosphate radicals in regenerated tobacco leaves through ion chromatography
  • Sample pretreatment method for simultaneous determination of oxalic acid, succinic acid, sulfuric acid and phosphate radicals in regenerated tobacco leaves through ion chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 The impact experiment of vitamin C on the determination of oxalic acid

[0039] At present, many ion chromatography methods for the determination of oxalic acid content in plants containing vitamin C, including tobacco, use NaOH solution as the eluent to separate oxalate and other anions. This study found that vitamin C can be converted to oxalate at high pH. Prepare 100ug / ml vitamin C standard solution with water as solvent, and use 10mmol / L, 20mmol / L, 30mmol / L NaOH solution for isocratic elution and 11mmol / L~50mmol / L NaOH solution gradient elution respectively on AS18 anion analysis column as Mobile phase procedure, measure vitamin C standard solution, all have oxalic acid group to go out peak on test chromatogram (retention time qualitative, see figure 1 shown). figure 2 Shown is the amount of oxalic acid produced when the 100ug / mL vitamin C standard solution prepared under two solvent bases of pure water and methanesulfonic acid is eluted with different...

Embodiment 2

[0040] The research experiment of embodiment 2 sample pretreatment method

[0041] The four target substances to be tested are all water-soluble anions. For ion chromatography detection, the commonly used extraction solvents are pure water, acid solution and sodium hydroxide solution. Due to the reason of 2.1, strong alkaline solution cannot be used for extraction. In the test, pure water was used as the extraction solvent to extract the reconstituted tobacco leaf samples and the residue after the first extraction by shaking for 30 minutes, and four target components were determined. The results show that using pure water as the extractant, the extraction efficiency of oxalic acid is only 37.7%, which cannot be extracted completely at one time. Since the analytical column used in this method is an anion column, commonly used acid ions will cause anion interference, so different concentrations of methanesulfonic acid (MSA) were used as the extraction solvent in the experiment....

Embodiment 3

[0044] The determination experiment of embodiment 3 chromatographic conditions

[0045] Based on the summary of long-term research experiments of the present invention and the aforementioned reasons, NaOH solution cannot be used as the eluent, and the test explores the use of NaCO 3 +NaHCO 3 Possibility as mobile phase, the results show (see Figure 5 ), using 4.5mmol / LNaCO 3 +1.4mmol / LNaHCO 3 As eluent, vitamin C standard solution was not converted into oxalic acid. At the same time, a variety of chromatographic columns were studied and investigated, such as AS14, AS22 and other chromatographic columns, and multiple factors such as resolution, peak shape symmetry, peak width and sensitivity were compared and analyzed, and AS22 was preferably determined as the analytical column of this method. The two target components are well separated on this column; the influence of mobile phase flow rate and injection volume is compared, the flow rate is 1.2ml / min, increasing the inje...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a sample pretreatment method for simultaneous determination of oxalic acid, succinic acid, sulfuric acid and phosphate radicals in regenerated tobacco leaves through the ion chromatography. According to the method, regenerated tobacco leaf samples are subjected to vibrating extraction by using methanesulfonic acid as extraction liquid and then are filtered by a filter membrane, and filter liquor, namely liquor to be detected, is collected. By the adoption of the method, the methanesulfonic acid is used as the extraction liquid, it is guaranteed that the extraction efficiency of all the components to be detected in the samples can reach 99.7%, and realizing of the technical scheme that the four components of the oxalic acid, the succinic acid, the sulfuric acid and phosphoric acid in the regenerated tobacco leaves can be detected at the same time accurately is effectively ensured. The method is simple, reliable and suitable for determining the related components in tobacco samples and the regenerated tobacco leaf samples at the same time.

Description

technical field [0001] The technical field of detection of reconstituted tobacco leaves of the present invention, more specifically, relates to a sample pretreatment method for simultaneous determination of oxalic acid, succinic acid, sulfuric acid and phosphate in reconstituted tobacco leaves by ion chromatography. Background technique [0002] Paper-making reconstituted tobacco has been increasingly used because of its advantages of balancing tobacco structure, stabilizing cigarette quality, and effectively reducing the release of tar and some harmful components. During the processing of reconstituted tobacco leaves by the papermaking method, various auxiliary agents need to be added, wherein succinic acid (succinic acid) and oxalic acid are used as process auxiliary agents, and phosphoric acid is added as an extraction auxiliary agent. Based on the stable quality and safety considerations of reconstituted tobacco products, the addition of various additives should be stric...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/88G01N30/06
Inventor 施文庄胡静李峰
Owner CHINA TOBACCO GUANGDONG IND
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com