A molecularly imprinted piezoelectric sensor for detecting trace citrinin and its preparation method

Abstract

The invention provides a molecular imprinting piezoelectric sensor for detecting trace citrinin and a preparation method of the molecular imprinting piezoelectric sensor. A composite film on the surface of an electrode of the sensor is gold nanoparticle@mesoporous carbon CMK-3 composite and a molecular imprinting polymer, the gold nanoparticle@mesoporous carbon CMK-3 composite film has a large specific surface area and a three-dimensional ordered mesoporous structure, and the molecular imprinting polymer can provide molecular imprinting holes corresponding to citrinin molecules. During preparation, a quartz crystal gold electrode is coated with the gold nanoparticle@mesoporous carbon CMK-3 composite and then put into an electrolyte solution containing dummy templates and functional monomers to have an electrochemical polymerization reaction, the dummy templates are removed after the reaction ends, and the molecular imprinting piezoelectric sensor is obtained and used for detecting citrinin. The preparation method is simple, the cost is low, and the molecular imprinting piezoelectric sensor has good selectivity, repeatability and anti-interference capacity and is successfully applied to detection of citrinin in an actual sample.

Description

technical field [0001] The invention relates to a piezoelectric sensor based on molecular imprinting technology, in particular to a molecular imprinting piezoelectric sensor for detecting trace citrinin and a preparation method thereof. Background technique [0002] Citrinin (Cit) is a toxic secondary metabolite produced by some filamentous fungi, Monascus, Aspergillus and Penicillium, often contaminating grain, food, feed, biological fluids, etc. In addition to strong nephrotoxicity, studies have found that citrinin also has carcinogenic, mutagenic, and teratogenic effects. So far, the qualitative and quantitative methods of citrinin mainly include high-performance liquid chromatography, thin-layer chromatography, enzyme-linked immunosorbent assay, liquid chromatography-mass spectrometry, and zone of inhibition method. However, due to the poor stability of citrinin in food and the lack of suitable and accurate analysis methods, there is no unified standard in the world. T...

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Problems solved by technology

However, because the molecularly imprinted polymer membranes prepared by electropolymerization are usually dense, the effective imprinted sites are limited.