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Application of berberine in the preparation of marine fouling biological control agent

A biological control agent, marine fouling technology, applied in the direction of biocide, chemicals for biological control, application, etc., to achieve good application prospects, great promotion potential, and environmentally friendly effects

Active Publication Date: 2017-06-23
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the field of marine fouling biological control, there is no relevant report disclosing its anti-adhesion effect and its use in fouling biological control

Method used

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  • Application of berberine in the preparation of marine fouling biological control agent
  • Application of berberine in the preparation of marine fouling biological control agent
  • Application of berberine in the preparation of marine fouling biological control agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Experimental group: dissolving berberine with distilled water to prepare a solution with a concentration of 28.26 μg / ml. Add 1 ml of this solution to a petri dish with a diameter of 6 cm, and make it evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the dose of berberine coated on the bottom of the culture dish is 10 μg / cm 2 . Add 10ml of sea water.

[0025] Control group: Add 1ml of distilled water to make it evenly distributed on the bottom of the culture dish, and then add 10ml of seawater after the volatilization is complete.

[0026] Blank group: add 10ml of seawater.

[0027] Five parallel samples were set up in each of the experimental group, the blank group and the control group. Add 30 netted barnacle Venus larvae to each sample. Place them in a constant temperature incubator at a temperature of about 29°C and cultivate them in a dark environment. Observed every 24 hours. Statistical analysis was carried out on the fi...

Embodiment 2

[0033] Experimental group: dissolving berberine with distilled water to prepare a solution with a concentration of 28.26 μg / ml. Add 1 ml of this solution to a petri dish with a diameter of 6 cm and make it evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the content of berberine coated on the bottom of the culture dish is 1.0 μg / cm 2 . Add 10ml of seawater.

[0034] Control group: Add 1ml of distilled water to distribute the solution evenly on the bottom of the culture dish, and then add 10ml of seawater after the solvent evaporates completely.

[0035] Blank group: add 10ml of seawater.

[0036] Determination of the number of larvae: Take the water body containing the larvae of the emerald mussel vectoli from the nursery pond and concentrate it with sieve silk. Take 50ml each for three times, add 1-2 drops of formalin solution to kill the larvae, and then count them under a microscope. The average value obtained is the density of larv...

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Abstract

The invention discloses the application of berberine in the preparation of marine fouling biological control agent. The structural formula of the berberine is shown in formula (I). When the berberine of the present invention is coated on a solid surface in a low dose, it has the effect of obviously inhibiting the attachment of barnacles and mussel larvae, so it can be used to prepare marine fouling biological control agents. And it is a naturally occurring organic compound, which will not pollute the water environment and cause its enrichment in organisms through the food chain. It is environmentally friendly and has high safety. It does not contain copper and tin while effectively inhibiting the adhesion of marine organisms. Heavy metal elements such as heavy metals have good social benefits from the perspective of environmental protection, and their sources are extensive, the separation and preparation methods are simple, suitable for large-scale production, and have great potential for promotion. They have good application prospects in marine biofouling control.

Description

[0001] Technical field: [0002] The invention belongs to the field of natural products, and in particular relates to the application of berberine in the preparation of marine fouling biological control agents. [0003] Background technique: [0004] Marine fouling organisms refer to all animals, plants and microorganisms that fix or inhabit underwater parts of ships and artificial facilities, and have adverse effects on human economic activities. The harm is mainly to increase the resistance of ships, increase fuel consumption, and reduce speed. Affect the maneuverability and combat effectiveness of ships; block water pipes, hinder water supply or reduce cooling effect; change metal corrosion process, cause local corrosion or perforation corrosion; reduce the sensitivity of instruments and rotating parts, interfere with the normal operation of instruments and equipment; increase the Self-weight and increase its center of gravity, significantly increase the dynamic load effect ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/90A01P9/00A01P7/00
Inventor 严涛黄日明曹文浩蒋小飞张慧陈殷宁陈迪
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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