Chlamydia pneumoniae quantum dot immunochromatographic assay detection card and preparing method and application thereof

Abstract

The invention provides a chlamydia pneumoniae quantum dot immunochromatographic assay detection card and a preparing method and application thereof. The detection card comprises a bottom board, a sample pad, a water absorbing pad, a combining pad and a detection layer. Chlamydia pneumoniae resisting nanometer probes labeled with quantum dots envelop combining pad. The detection layer is formed by a solid-phase nitrocellulose membrane with a detection line and a quality control line. Anti-mouse chlamydia pneumoniae 98KD membrane protein polyclonal antibodies envelop the detection line. Anti-rabbit IgG is encapsulated in the quality control line. The detection layer is bonded to the bottom board. The combining pad and the water absorbing pad are arranged on the upper portions of the two ends of the detection layer respectively, partially overlapped with the detection layer and then bonded to the detection layer and the bottom board. The sample pad is arranged on the combining pad, partially overlapped with the combining pad and then bonded to the combining pad and the bottom board. The chlamydia pneumoniae quantum dot immunochromatographic assay detection card has the advantages of being easy and convenient to operate, fast in detection, capable of achieving quantification, high in sensitivity and the like.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a human Chlamydia pneumoniae quantum dot immunochromatographic detection card and its preparation method and application. Background technique [0002] Chlamydiapneumoniae (Chlamydiapneumoniae, Cpn), as an important respiratory pathogenic microorganism of the genus Chlamydia, was officially named by Grayston et al. in the mid-1980s, and has been extensively studied by researchers in the subsequent time. It is only known that humans are the host of the pathogen, and the infection may be transmitted from person to person through respiratory secretions. Children under the age of 5 are rarely infected, and children over the age of 8 and young people are susceptible to infection, especially in places where people gather, such as families, schools, and barracks. Serological epidemiological investigations have confirmed that at least 40% of adults have been infected. Infected...

AI Technical Summary

Problems solved by technology

However, the PCR experiment has special requirements for the laboratory, the sample processing, amplification and detection requirements are strict, and it is prone to false positives, so it cannot be used as a commonly used clinical diagnostic method in my country.

[0005] Antigen detection methods include separation and culture of chicken embryo yolk sac and cell culture method (two kinds of cells, HL and Hep-2, are commonly used), and then use fluorescent-labeled or enzyme-labeled antibodies to detect Chlamydia in the specimen, but this method has complicated operation steps and cell culture Obvious defects such as long time, not suitable for clinical application

In recent years, it has also been tried to be directly applied to the detection of clinical specimens, and its sensitivity is related to specimen collection: EIA can be used to detect the presence of Chlamydia pneumoniae antigen after smears of sputum and throat swabs, but its sensitivity, specificity and reproducibility are not ideal , and is limited to acute respiratory infection; enzyme-linked immunosorbent assay (ELISA) can also directly detect pathogens, mainly detecting Chlamydia pneumoniae outer membrane protein-2, the antibody used is an anti-Chlamydia specific antibody, which cannot directly recognize Chlamydia pneumoniae, and Sensitive but less specific than the "gold standard" (MIF)

Images