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Method for extracting chicoric acid from echinacea purpurea moench

A technology of Echinacea purpurea and cichoric acid, applied in the separation/purification of carboxylic acid esters, organic chemistry, etc., can solve the problems of low purity and yield of cichoric acid, and achieve the effect of improving the purity and yield

Inactive Publication Date: 2016-02-03
GUILIN SANBAO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main preparation method of cichoric acid is to reflux extraction with ethanol, and then to purify with macroporous resin, but the purity and yield of cichoric acid extracted by the above-mentioned method are all low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1) Take 100 g of the dry root product of Echinacea purpurea and extract it with 60v% ethanol, the amount of ethanol is 10 times the weight of Echinacea purpurea, reflux extraction at 40°C for 3 hours, filter the extract, and take the filtrate for later use;

[0019] 2) Add activated carbon to the filtrate for decolorization, the amount of activated carbon added is 1‰ of the total weight of the filtrate, the decolorization temperature is 40°C, and the decolorization time is 20min, filter to obtain a clear liquid;

[0020] 3) After concentrating the clear solution, apply silica gel column chromatography, the flow rate of the concentrated solution is 0.5BV / h; wash the column with water until it is colorless, the flow rate of water is 0.5BV / h, and the dosage is 1BV; then use a mixture of methanol and acetone Solvent for elution, the volume ratio of methanol and acetone is 50:40, the elution fluidity is 1BV / h, the dosage is 2BV, and the eluent of methanol and acetone is colle...

Embodiment 2

[0023] 1) Take 100 g of dry root product of Echinacea purpurea and extract with 75v% ethanol, the amount of ethanol is 30 times of the weight of Echinacea purpurea, reflux extraction at 60° C. for 2 hours each time, combine the extracts, filter, and take the filtrate for later use;

[0024] 2) Add activated carbon to the filtrate for decolorization, the amount of activated carbon added is 1.5‰ of the total weight of the filtrate, the decolorization temperature is 60°C, the decolorization time is 30min, filter to obtain a clear liquid;

[0025] 3) After concentrating the clarified solution, go to silica gel column chromatography, the flow rate of the concentrated solution is 1BV / h; the column is washed with water until it is colorless, the flow rate of water is 1BV / h, and the dosage is 2BV; then use a mixed solvent of methanol and acetone For elution, the volume ratio of methanol and acetone is 50:50, the elution fluidity is 1.5BV / h, the dosage is 2.5BV, and the eluent of methan...

Embodiment 3

[0028] 1) Take 100 g of the dry root product of Echinacea purpurea and extract it with 65v% ethanol. The amount of ethanol is 20 times the weight of Echinacea purpurea. Reflux extraction at 50° C. for 2 hours each time, combine the extracts, filter, and take the filtrate for later use;

[0029] 2) adding activated carbon to the filtrate for decolorization, the amount of activated carbon added is 1‰ of the total weight of the filtrate, the decolorization temperature is 50°C, the decolorization time is 25min, and the clarified liquid is obtained by filtering;

[0030] 3) After concentrating the clarified liquid, go to silica gel column chromatography, the flow velocity of the concentrated solution is 0.5BV / h; the column is washed with water until colorless, the flow velocity of water is 0.5BV / h, and the consumption is 1.5BV; then use methanol and acetone Mixed solvent for elution, the volume ratio of methanol and acetone is 60:40, the elution fluidity is 1BV / h, the dosage is 2BV,...

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PUM

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Abstract

The invention provides a method for extracting chicoric acid from echinacea purpurea moench. The method comprises the following steps: 1) extracting the echinacea purpurea moench by 60 to 75v% ethyl alcohol, filtering an extracting solution, and taking filtrate for standby; 2) adding activated carbon into the filtrate to carry out decoloration, and filtering to obtain supernatant liquid; 3) after the supernatant liquid is concentrated, putting a silica gel column to carry out chromatography, washing the column with water until the column is colorless, then, carrying out elution by a mixed solvent of methyl alcohol and acetone, and collecting a eluent of the methyl alcohol and the acetone; 4) carrying out the chromatography on the eluent of the methyl alcohol and the acetone on the silica gel column again, eluting by the mixed solvent of the ethyl alcohol, the acetone and water, collecting the eluent of the ethyl alcohol, the acetone and the water, recovering the solvent, and drying to obtain the chicoric acid. According to the method, the yield and the purity of the chicoric acid can be greatly improved, the yield of an echinacea purpurea moench dried product can reach not less than 1.50% through detection, and purity can reach not less than 96.5%.

Description

technical field [0001] The invention belongs to the technical field of plant activity extraction, and in particular relates to a method for extracting cichoric acid from Echinacea purpurea. Background technique [0002] Echinacea purpurea (Echinacea purpurea Moench) belongs to the Asteraceae Echinacea genus herbaceous perennials. Chichoric acid is a derivative of caffeic acid and is one of the most important immune active components in Echinacea purpurea. Chichoric acid can inhibit hyaluronidase and HIV-1 integrase, and has cell phagocytosis promoting effect and antiviral activity in vitro and in vivo. After testing, the content of cichoric acid in the dried root of Echinacea purpurea is 1.78g / 100g, while the content of cichoric acid in the aerial part is 1.69g / 100g. At present, the main preparation method of cichoric acid is to first use ethanol to heat and reflux to extract, and then to purify it with a macroporous resin. However, the purity and yield of cichoric acid ex...

Claims

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Application Information

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IPC IPC(8): C07C69/732C07C67/48
CPCC07C67/48C07C69/732
Inventor 周晓青钟德品谢冬养陆美珍赵明东
Owner GUILIN SANBAO PHARMA
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