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Preparation method of cell membrane microcapsule simultaneously loaded with chemotherapeutic drug and photodynamic therapeutic drug

A photodynamic therapy and chemotherapeutic drug technology, applied in microcapsules, drug combinations, pharmaceutical formulas, etc., can solve the problems of difficult to reach the lesion, short circulation time in the body, limit promotion and application, etc., to achieve increased permeability, superior Biocompatibility and delivery performance, the effect of fast preparation speed

Active Publication Date: 2016-02-03
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, they often have poor biocompatibility, are easier to be recognized and eliminated by the human immune system, and have the disadvantages of short circulation time in the body and difficult to reach the lesion site, which limit their promotion and application.

Method used

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  • Preparation method of cell membrane microcapsule simultaneously loaded with chemotherapeutic drug and photodynamic therapeutic drug
  • Preparation method of cell membrane microcapsule simultaneously loaded with chemotherapeutic drug and photodynamic therapeutic drug
  • Preparation method of cell membrane microcapsule simultaneously loaded with chemotherapeutic drug and photodynamic therapeutic drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) Add 20μL of cytochalasin solution with a concentration of 1mg / mL to the culture plate for culturing human endothelial cells, and incubate for 30min at 37℃; wash three times with physiological saline, digest with trypsin, centrifuge at 500rpm for 10min to remove the cells, and the supernatant is It is a suspension of cell membrane microcapsules;

[0027] 2) Take 200 μg of the cell membrane microcapsules obtained in step 1) and add it to 1 mL of a mixed solution of digitalis saponin and the chemotherapy drug doxorubicin hydrochloride. The concentration of digitalis saponin in the mixed solution is 10 μg / mL and the concentration of doxorubicin hydrochloride is 200 μg / mL; incubate at 37°C for 6h;

[0028] 3) Centrifuge the cell membrane microcapsules obtained in step 2), wash off excess doxorubicin hydrochloride with saline; add 1 mL of photodynamic therapy drug indocyanine green solution, the concentration of indocyanine green in the solution is 200μg / mL; 37 Incubate at ℃ ...

Embodiment 2

[0033] Same as Example 1, the difference is that in step 2), the 37°C incubation time is 1 h.

Embodiment 3

[0035] Same as Example 1, the difference is that in step 2), the concentration of the chemotherapeutic drug doxorubicin hydrochloride in the mixed solution is 20 μg / mL.

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Abstract

The invention discloses a cell membrane microcapsule simultaneously loaded with a chemotherapeutic drug and a photodynamic therapeutic drug. Human cells are selected as a raw material for preparing the cell membrane microcapsule; the obtained cell membrane microcapsule is soaked into a digitonin solution to improve the permeability of cell membranes; a chemotherapeutic drug namely doxorubicin hydrochloride and a photodynamic therapeutic drug namely indocyanine green are added sequentially to ensure that the drugs are infiltrated into the cell membrane microcapsule; and calcium ions are used for sealing the cell membranes to obtain the cell membrane microcapsule simultaneously loaded with the chemotherapeutic drug and the photodynamic therapeutic drug. The cell membrane microcapsule can generate active oxygen under the irradiation of laser light of 808nm, and the active oxygen and the chemotherapeutic drug act together to kill tumor cells, so that the cell membrane microcapsule is expected to be used for tumor treatment. The preparation method of the cell membrane microcapsule disclosed by the invention is simple, convenient and controllable, is high in production efficiency and has good application prospects.

Description

Technical field [0001] The invention relates to a method for preparing cell membrane microcapsules simultaneously loaded with chemotherapeutic drugs and photodynamic therapy drugs. Background technique [0002] Photodynamic therapy is a new type of treatment that uses a combination of laser and photodynamic therapy drugs (photosensitizers) to specifically kill diseased tissues. After laser stimulating photodynamic therapy drugs, around O 2 It is transformed into reactive oxygen species such as singlet oxygen, and the reactive oxygen kills target cells through oxidation. However, singlet oxygen, which plays a major role in killing, has a short survival time. Realizing the positioning effect of photodynamic therapy drugs is one of the key contents of photodynamic therapy research, and its delivery through a carrier is a way to achieve this function. [0003] Chemotherapy is also a common method to treat tumors and other diseases. Doxorubicin is an anthracycline anti-tumor antibioti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/50A61K31/704A61P35/00
Inventor 毛峥伟韩利杰高长有
Owner ZHEJIANG UNIV
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