Lipoprotein phospholipase A2 (Lp-PLA2) immunofluorescence probe detection kit

Abstract

The present invention discloses a self-designed immunofluorescence probe detection system for quantitative detection of risk predictive factor Lp-PLA2 of arteriosclerosis cerebral infarction for predicting whether a patient may be subjected to atheroma heart disease. The immunofluorescence probe detection system comprises a sample collection system, a reaction detection system, a laser emission system, a fluorescence collection system and a fluorescence conversion system. The probe is connected with a negative-pressure device to successively enter a collected blood sample into a blood filter device for filtering the blood samples, then into a sample boding zone to combine with fluorescent microsphere labeled antibodies, and finally into a sample detection zone to combine with coated antibodies, and namely, the blood sample exists in Ab-Ag (sample)-Ab (fluorescent microsphere labeled) sandwich complex form. By laser irradiating, the labeled antibodies are excited to produce fluorescence, a fluorescence signal is transferred to a fluorescence photosensitive area by a fluorescence conduction device, and the fluorescence signal is converted into a digital signal, and then converted into a sample concentration signal. The characteristics of the self-designed immunofluorescence probe detection system are that: the probe is used for rapid detection of the heart disease predictive factor Lp-PLA2, the collection of blood samples to detection of the results can be completed within 5 minutes, detection is rapid and accurate, operation is simple, and the risk of sudden heart attack of the patient can be earlier and faster forecasted. The immunofluorescence probe detection system is a separable device, and can pass through the design of different projects.

Description

technical field [0001] The present invention is based on a self-designed immunofluorescence probe detection system to quantitatively detect the risk predictor Lp-PLA2 of arteriosclerosis and cerebral infarction, which belongs to the field of medical products. Background technique [0002] Coronary artery disease, also known as coronary heart disease (CHD), is a heart disease caused by atherosclerotic lesions in the coronary arteries that cause stenosis or blockage of the vessel lumen, resulting in myocardial ischemia, hypoxia or necrosis. The occurrence and development of coronary atherosclerosis is a chronic inflammatory process. Chronic inflammation is considered to increase the risk factors of coronary artery disease and make atherosclerotic plaques prone to rupture. In recent years, studies have found that lipoprotein-associated phospholipase A2 is a new predictor of coronary heart disease, and pointed out that it is closely related to atherosclerotic lesions. Lp-PLA2, ...

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Problems solved by technology

However, it is still impossible to warn of the risk of myocardial infarction at any time due to the aggravation of atherosclerotic plaque inflammation in blood vessels

[0006] 3) The detection of electrocardiogram and various myocardial enzymes is an abnormal index that occurs after myocardial infarction, so it loses the meaning of prevention

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