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Method for extracting holothurian saponins by using holothurian blanching liquid

The technology of sea cucumber saponins and blanching liquid is applied in the field of extracting active substances, which can solve the problems of low saponin adsorption efficiency, low saponin content and purity, weak adsorption specificity, etc., and achieves high health care value, extensive social and economic benefits, The effect of increasing operability and practicality

Active Publication Date: 2016-01-27
SHANDONG HOMEY AQUATIC DEV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the deficiencies in the prior art of directly using macroporous adsorption resins to adsorb saponins in sea cucumber blanching liquid, the adsorption efficiency is low, the adsorption specificity is not strong, the column is easy to be blocked, and the purity of saponins is low. Method for extracting sea cucumber saponins from sea cucumber blanching liquid with feasible operation, low production cost, high product yield and purity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for extracting sea cucumber saponins from sea cucumber blanching liquid, which undergoes the following process steps:

[0029] (1) Selection and processing of raw materials

[0030] Sea cucumber blanching liquid is selected as raw material, and it is concentrated under reduced pressure at 100 atmospheres to a solid content of 30% to obtain a concentrated liquid; wherein, the sea cucumber blanching liquid is fresh sea cucumbers that have been dissected and guts removed, and put into boiling water. Boil for 20 minutes, remove the liquid obtained from the sea cucumber;

[0031] (2) spray drying

[0032] Spray drying the concentrate obtained in step (1) to obtain a first solid powder;

[0033] (3) Degreasing

[0034] Using the first solid powder obtained in step (2) with supercritical CO 2 Fluid extraction, remove grease, get the second solid powder; among them, supercritical CO 2 The temperature of fluid extraction is controlled at 45°C, the pressure is 35Mpa...

Embodiment 2

[0047] A method for extracting sea cucumber saponins from sea cucumber blanching liquid, which undergoes the following process steps:

[0048] (1) Selection and processing of raw materials

[0049] Sea cucumber blanching liquid is selected as raw material, and it is concentrated under reduced pressure at 70 atmospheres to a solid content of 45% to obtain a concentrated liquid; wherein, the sea cucumber blanching liquid is fresh sea cucumbers that have been dissected and guts removed, and put into boiling water. Boil for 30 minutes, remove the liquid obtained from the sea cucumber;

[0050] (2) spray drying

[0051] Spray drying the concentrate obtained in step (1) to obtain a first solid powder;

[0052] (3) Degreasing

[0053] Using the first solid powder obtained in step (2) with supercritical CO 2 Fluid extraction, remove grease, get the second solid powder; among them, supercritical CO 2 The temperature of fluid extraction is controlled at 50°C, the pressure is 40Mpa,...

Embodiment 3

[0066] A method for extracting sea cucumber saponins from sea cucumber blanching liquid, which undergoes the following process steps:

[0067] (1) Selection and processing of raw materials

[0068] Sea cucumber blanching liquid is selected as the raw material, and it is concentrated under reduced pressure at 200 atmospheres until the solid content is 20% to obtain a concentrated liquid; wherein, the sea cucumber blanching liquid is fresh sea cucumbers that have been dissected and removed from the intestines, and then put into boiling water Boil for 10 minutes, remove the liquid obtained from the sea cucumber;

[0069] (2) spray drying

[0070] Spray drying the concentrate obtained in step (1) to obtain a first solid powder;

[0071] (3) Degreasing

[0072] Using the first solid powder obtained in step (2) with supercritical CO 2 Fluid extraction, remove grease, get the second solid powder; among them, supercritical CO 2 The temperature of fluid extraction is controlled at...

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Abstract

The invention discloses a method for extracting holothurian saponins by using a holothurian blanching liquid. According to the method, the holothurian blanching liquid is subjected to concentration under reduced pressure and spray-drying and then subjected to extraction by using a supercritical CO2 fluid, solid powder with grease removed is obtained, subjected to alcohol extraction, suction filtration and concentration and then re-dissolved in water, compounds such as phospholipids, cerebrosides and the like in a solution are removed with a centrifugal method, then an obtained supernate is subjected to adsorption chromatography by the aid of a macroporous adsorption resin column, elution is performed by using an ethanol solution, an elution solution is subjected to vacuum freeze-drying, and a holothurian saponin extract is obtained. With the adoption of the method, the adsorption chromatography efficiency of the macroporous adsorption resin column is improved, the service life of the resin column is prolonged, the extracting method adopts the reasonable technology, operation is simple, the production cost is low, continuous production can be realized, and the purity and the yield of target products are increased.

Description

technical field [0001] The invention relates to a technology for extracting active substances from sea cucumber processing by-products, in particular to a method for extracting sea cucumber saponins from sea cucumber blanching liquid. Background technique [0002] After fresh sea cucumbers are harvested, due to the presence of proteolytic enzymes such as autolytic enzymes, it is easy to peel and autolyze. In order to facilitate preservation, fresh sea cucumbers are usually processed and stored after blanching in hot water. However, fresh sea cucumbers produce a large amount of blanching liquid in the process of hot water treatment, and it is found through testing that the blanching liquid of sea cucumbers contains a large amount of active substances, such as sea cucumber saponins. Sea cucumber saponins are a class of main active ingredients in sea cucumbers, which have a variety of pharmacological activities, such as anti-tumor effects, improving immunity, anti-fungal, anti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L17/20A23L33/00
CPCA23V2002/00A61K35/616A23V2250/2042
Inventor 袁文鹏刘昌衡孙永军张绵松史亚萍夏雪奎鞠文明乔若瑾胡炜唐晓波刘新贾爱荣张永刚齐君
Owner SHANDONG HOMEY AQUATIC DEV
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