Screening method of resisting joint toxicity of heavy metals by trisodium citrate dihydrate

Abstract

The invention discloses a screening method of resisting joint toxicity of heavy metals by trisodium citrate dehydrate. The screening method comprises the following steps: step one, preparing a mixed solution of multiple heavy metal pollutants; step two, determining LC50 of the heavy metal mixed solution by MTT; step three, by taking trisodium citrate dehydrate as an antagonist, sequentially mixing trisodium citrate dehydrate water solutions with different concentrations to be screened and the heavy metal mixed solution with concentration capable of causing the death of half number of cells, adding the mixture into a culture medium to be used as a cell line to be cultured; and step four, testing the survival activity of MTT cells, namely, poisoning for 24h, adding MTT, incubating for 3-4h, and measuring the survival activity of the cells, thus obtaining the resisting efficiency of the chemical antagonists with different concentrations to be screened. The screening method has the advantages that the screening rapid is high, the cost is low, and the antagonists with different concentrations can be screened simultaneously, so that the screening efficiency is high.

Description

technical field [0001] The invention relates to the technical field of heavy metal pollutant repellent, in particular to the repellent combined toxicity of various heavy metal pollutants, in particular to a screening method for trisodium citrate dihydrate to antagonize the combined toxicity of heavy metal pollutants. Background technique [0002] Heavy metals generally refer to metal elements with a specific gravity greater than 4.0. Heavy metals or their compounds are a class of pollutants that are difficult to degrade in the environment. They are widely present in the atmosphere, water, soil and other environmental media and cause serious environmental pollution. Heavy metal pollution refers to environmental pollution caused by heavy metals or their compounds. In recent years, there have been frequent incidents of heavy metal pollution in my country, ranging from excessive blood lead in children to heavy metal pollution of "cadmium rice" and so on. Heavy metal pollution ha...

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Problems solved by technology

In addition, studies have shown that almost all heavy metal ions can cause the generation of active oxygen groups in the body, which in turn leads to oxidative stress damage in cells and tissues

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