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Method for extracting blood sugar-reducing panaxan by enzyme method and application of obtained panaxan

A technology of ginseng polysaccharides and enzymatic extraction, which is applied in the directions of metabolic diseases, fermentation, drug combination, etc., can solve the problems of large loss of active ingredients, long extraction cycle, high solvent consumption, etc., to promote release, shorten extraction time, and improve extraction efficiency Effect

Inactive Publication Date: 2016-01-20
QIQIHAR MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are problems such as long extraction period, high solvent consumption, large loss of active ingredients, and high energy consumption.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] S1. Take clean ginseng and cut it into slices with a thickness of 5 mm, put it in the steam explosion tank, first pass nitrogen gas until the pressure in the steam explosion tank is 1 MPa, and blast for 15 minutes; then quickly inject steam to the pressure in the steam explosion tank 1.6MPa, steam explosion treatment for 1.6min, and dry to get ginseng powder;

[0027] S2, the ginseng powder obtained in step S1 is placed in an enzymolysis tank, and a biological compound enzyme preparation comprising mixed protease, pectinase, glucoamylase, lignin degrading enzyme, and laccase is added at a ratio of 85.0 ml / ton, 35 ℃, enzymolysis under the action of high-voltage pulsed electric field for 4 hours, the electric field strength is 30kv / cm, and the number of pulses is 8;

[0028] S3. Heat the solution after enzymolysis at 100°C for 6min to passivate it, add 72.5% natural source turpentine derivatives for leaching 2.5 times, the extraction temperature is 77.5°C, each time is 1 ...

Embodiment 2

[0031] S1. Take clean ginseng and cut it into 5 mm thick slices, put it in the steam explosion tank, first pass nitrogen into the steam explosion tank until the pressure in the steam explosion tank is 0.6MPa, and blast for 7 minutes; then quickly inject steam into the steam explosion tank The pressure is 1.4MPa, the steam explosion treatment is 0.6min, and dried to obtain ginseng powder;

[0032] S2, the ginseng powder obtained in step S1 is placed in an enzymolysis tank, and a biological compound enzyme preparation comprising mixed protease, pectinase, glucoamylase, lignin degrading enzyme, and laccase is added at a rate of 70.0 ml / ton, 30 ℃, under the action of high-voltage pulsed electric field for 3 to 5 hours, the electric field strength is 25kv / cm, and the number of pulses is 6;

[0033] S3. Heat the solution after enzymolysis at 100° C. for 5 minutes to passivate it, add turpentine derivatives with a concentration of 65% from natural sources to extract twice, the extrac...

Embodiment 3

[0036] S1. Take clean ginseng and cut it into 5 mm thick slices, put it in the steam explosion tank, first pass nitrogen into the steam explosion tank until the pressure in the steam explosion tank is 1.4MPa, and blast for 23 minutes; then quickly inject steam into the steam explosion tank The pressure is 1.8MPa, the steam explosion treatment is 2.6min, and dried to obtain ginseng powder;

[0037] S2. Place the ginseng powder obtained in step S1 in an enzymolysis tank, add a biological compound enzyme preparation comprising mixed protease, pectinase, glucoamylase, lignin degrading enzyme, and laccase at a rate of 100.0 ml / ton, 40 ℃, enzymatic hydrolysis for 5 hours under the action of high-voltage pulsed electric field, the electric field strength is 35kv / cm, and the number of pulses is 10;

[0038] S3. Heat the solution after enzymolysis at 100° C. for 7 minutes to passivate it, add turpentine derivatives from natural sources with a concentration of 80% for leaching 3 times, ...

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PUM

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Abstract

The invention discloses a method for extracting a blood sugar-reducing panaxan by an enzyme method and the application of the obtained panaxan. The method comprises the following steps of after slicing clean gen-seng into slices with the thickness of 5mm, performing steam explosion treatment on the slices, and drying the slices to obtain gen-seng powder; placing the obtained gen-seng powder in an enzymatic vessel, adding biologic composite enzyme preparations containing mixed protease, pectinase, glucose amylase, lignin-degrading enzyme and laccase into the vessel, and performing enzymolysis for 3 to 5 hours under the action of a high-pressure pulse field; heating the solution obtained after enzymolysis for 5 to 7 minutes at the temperature of 100 DEG C to passivate the solution, adding an organic solvent into the solution to leach for 2 to 3 times, performing centrifugal separation, and after mixing the supernatants, performing spray drying to obtain powder, wherein the drying temperature is not more than 65 DEG C, and the water content is controlled to be less than 7 percent. Due to the fact that in an enzymolysis reaction process, a steam explosion treatment technology and the high-pressure pulse field are introduced, the activity of the enzyme preparation is improved, the enzymolysis reaction speed is increased, and dissolution of saponin substances in the gen-seng is quickly and effectively promoted.

Description

technical field [0001] The invention relates to the technical field of polysaccharide extraction and application, in particular to a method for enzymatically extracting hypoglycemic ginseng polysaccharide and the application of the obtained polysaccharide. Background technique [0002] Ginseng (Panaxginseng C.A.Mey) is the dried root of Araliaceae plant ginseng, commonly known as ginseng, ghost cover, goblin, baby ginseng, etc. It is a precious medicinal material in my country and has a medicinal history of thousands of years. Ginseng is mild in nature, sweet in taste, slightly bitter and lukewarm. It has the effects of invigorating vital energy, recovering pulse and strengthening the spleen, nourishing the spleen and lung, promoting body fluid and quenching thirst, calming the nerves and improving intelligence. In recent years, scholars at home and abroad have conducted pharmacological studies, clinical observations and epidemiological examinations on the active ingredients...

Claims

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Application Information

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IPC IPC(8): C12P19/04A61K31/715A61P3/10
Inventor 于海涛张春晶孙艳
Owner QIQIHAR MEDICAL UNIVERSITY
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