Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation and applications of anti-Och1 monoclonal antibody

A monoclonal antibody, Candida albicans technology, applied in the field of medical bioengineering, can solve the problem of no anti-Och1 monoclonal antibody and so on

Active Publication Date: 2016-01-20
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are no literature reports on anti-Och1 monoclonal antibodies, and there are no literature reports on the use of anti-Och1 monoclonal antibodies against invasive Candida infections

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation and applications of anti-Och1 monoclonal antibody
  • Preparation and applications of anti-Och1 monoclonal antibody
  • Preparation and applications of anti-Och1 monoclonal antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1. Polypeptide sequence selection and preparation of polypeptides of anti-Och1 monoclonal antibodies

[0058] Selection of Peptide Sequences

[0059] Och1 has 385 amino acids. According to its amino acid sequence information, Abimate Biomedicine (Shanghai) Co., Ltd. was entrusted to design, screen and synthesize the peptide sequence for peptide synthesis: DWKLFTGMEQ

Embodiment 2

[0060] Example 2. Preparation and purification of monoclonal antibodies against Och1

[0061] Entrusted Abimat Biopharmaceutical (Shanghai) Co., Ltd. to carry out the preparation and purification of monoclonal antibody.

[0062] 1. Antigen Production

[0063] The whole gene synthesis of the polypeptide, the gene sequence refers to the Candida Genome Database. Construction of expression vectors; identification of positive clones; plasmid extraction of positive clones and transformation of expression bacteria; large-scale induced expression production; Ni column affinity purification; after purification, protein antigens were obtained for immunization.

[0064] 2. Preparation and purification of monoclonal antibodies

[0065] Three healthy female BALB / c mice aged 8-12 weeks were immunized with the successfully obtained antigen mixture. The spleens of three mice were mixed and fused by rapid immunization of mice.

[0066] The splenocytes of the mice with the best immune respons...

Embodiment 3

[0069] Example 3. Identification and detection application of monoclonal antibodies against Och1

[0070] 1. Western Blot detection application of monoclonal antibody

[0071] The denatured Candida albicans cell wall protein samples were subjected to SDS-PAGE protein electrophoresis; after the electrophoresis, the semi-dry transfer was performed. Add blocking solution and block at room temperature for at least 1 h; wash the membrane 5 times with PBST washing solution, use monoclonal antibody as the primary antibody, and incubate overnight at 4°C; +L) 1:10000 dilution and incubation at room temperature for 2 hours, with slight shaking during incubation; the membrane was washed 5 times with PBST washing solution, and the NC membrane was scanned with an Odyssey infrared imaging system. see results figure 1 . The results showed that the monoclonal antibody could specifically recognize the cell wall protein of Candida albicans.

[0072] 2 Immunofluorescence detection applicatio...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to the technical field of medical bioengineering, and provides an anti-Candida albicans cell wall protein Och1 monoclonal antibody, a polypeptide for preparing the monoclonal antibody, a hybridoma cell line for secreting the monoclonal antibody, and applications of the monoclonal antibody, the polypeptide and the hybridoma cell line in preparation of Western Blot or immunohistochemical Candida albicans infection detection reagents or kits, and in preparation of drugs for prevention or treatment of Candida albicans infections.

Description

technical field [0001] The invention relates to the technical field of medical bioengineering, in particular to an anti-Och1 monoclonal antibody and the application of the monoclonal antibody in anti-Candida albicans infection. Background technique [0002] OCH1 is a gene encoding cell wall α-1,6-mannosyltransferase. Deletion can block the hyperglycosylation of glycoproteins. It is necessary for the virulence of Candida albicans and is a fungal specific gene. Therefore, the Och1 protein is a promising antifungal target for antibody-based therapy. [0003] At present, there is no literature report on anti-Och1 monoclonal antibody, and there is no literature report on the use of anti-Och1 monoclonal antibody to resist invasive Candida infection. Contents of the invention [0004] The object of the present invention is to provide an anti-Candida albicans cell wall protein Och1 monoclonal antibody, a polypeptide for preparing the monoclonal antibody, a hybridoma cell line sec...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/10C12N5/20C07K16/40G01N33/577G01N33/569A61K39/395A61P31/10C12R1/91
Inventor 阎澜张艳霞王晓娟王丽姜远英
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com