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Method for producing bio-organic fertilizer by utilizing pig manure and mushroom slag

A bio-organic fertilizer and organic fertilizer technology, applied in the direction of microbial-based methods, preparation of organic fertilizers, bio-organic part treatment, etc., can solve problems such as difficult to control microorganisms, inconvenience, etc., achieve reduction of pests and diseases, simple components, and improved The effect of soil structure

Inactive Publication Date: 2018-04-03
HUNAN AGRICULTURAL UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

For example, the Institute of Plant Protection Soil and Fertilizer of the Hubei Academy of Agricultural Sciences applied for an invention patent (Application No. CN201210307075) of "a double-effect bio-organic fertilizer for preventing and treating soil-borne diseases of crops and its production method". There are more than 10 kinds of strains. Due to the large number of strains, it is difficult to control the ebb and flow in the microbial fermentation process, which is not convenient for the actual operation of agricultural production.

Method used

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  • Method for producing bio-organic fertilizer by utilizing pig manure and mushroom slag
  • Method for producing bio-organic fertilizer by utilizing pig manure and mushroom slag
  • Method for producing bio-organic fertilizer by utilizing pig manure and mushroom slag

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Experimental program
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Effect test

Embodiment 1

[0034] Embodiment 1, Bacillus subtilis Lys-979 obtains

[0035] The high-temperature-resistant Bacillus Lys-979 was isolated from corn stalks, rice straw, and tobacco piles in Yunyuan, Hunan Agricultural University. After morphological identification and 16S RNA evolution tree molecular identification, the strain Lys-979 was Bacillus subtilis (Bacillus subtilis). Bacillus subtilis Lys-979 was deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms on September 8, 2015, and the preservation number is CGMCC No.11333.

Embodiment 2

[0036] Embodiment 2, the preparation of Bacillus subtilis Lys-979 liquid fermentation bacterial agent

[0037] Inoculate one loop of strain Lys-979 test tube slant into four 500mL Erlenmeyer flasks. The medium formula is 0.5% yeast extract, 2.0% glucose, pH 7.0, 250mL water, sterilized at 121°C for 25min; fermented at 40°C at 200rpm for 12~ It takes 18 hours, and the number of bacteria reaches 5-10 billion / mL, and it is prepared into shake flask seeds; then 1000mL of strain Lys-979 shake flask seed solution is inoculated to 15L for fermentation according to 1:10 (v / v) aseptic operation In the tank, the formula of the medium is 0.5% of yeast extract, 2.0% of glucose, 0.03% of GP330 defoamer, pH 7.0, 10L of tap water, about 75% of liquid content, sterilized at 121°C for 25 minutes, ventilation (1:1, v / v), 40°C, 200rpm fermentation for 12-15 hours, the number of bacteria reached 5-10 billion / mL, prepared into first-class seeds; further 10L of strain Lys-979 first-class seeds, ac...

Embodiment 3

[0038] Embodiment 3, the preparation of Bacillus subtilis Lys-979 solid fermentation bacterial agent

[0039] Inoculate one loop of strain Lys-979 test tube slant into four 500mL Erlenmeyer flasks, several, the medium formula is 0.5% yeast extract, 2.0% glucose, pH 7.0, 250mL water, sterilized at 121°C for 25min; fermented at 40°C, 200rpm It takes 12-18 hours, and the number of bacteria reaches 5-10 billion / mL, and prepared into shake flask seeds; then inoculate 1000mL of strain Lys-979 shake flask seed solution according to 1:40 (w / w) aseptic operation to In 200 bottles of 500mL triangular flasks, each bottle contains 100g of culture medium; the medium formula is 25% bran, 25% mushroom dregs, 50% water, natural pH, sterilized at 121°C for 30min; fermented for 24-36 hours at 37°C , the number of bacteria reached 10-20 billion / g, and prepared into first-class solid seeds; finally, the strain Lys-979 first-class solid seeds were aseptically inoculated to 200 plates of 40cm×60cm×...

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Abstract

The invention provides microorganism bacillus subtilis Lys-979 for efficiently and quickly fermenting pig manure and mushroom residues and a technological method for preparing bio-organic fertilizer through fermentation of microorganism bacillus subtilis Lys-979. The preservation serial number of microorganism bacillus subtilis Lys-979 is CGMCC No.11333, according to the pig manure and the mushroom residues fermented through the strain, fermentation base materials are quickly decomposed in 18-22 days, and the physical and chemical indicators meet the new standard of organic fertilizer; on the basis, a functional anti-biological inoculant is compounded, the bio-organic fertilizer with the control function on pest and disease damage of crops is prepared, the problems that at present, pollution is caused by excrement in production and husbandry and waste straw residues, and compost is long in fermentation time and high in cost are fundamentally solved, and the sustainable development of agricultural production is promoted.

Description

technical field [0001] The invention relates to a production method for obtaining bio-organic fertilizer by using agricultural and animal husbandry waste to turn waste into treasure, and belongs to the field of organic fertilizer biotechnology. Background technique [0002] Pig farming is the largest pillar industry of animal husbandry in my country, and pork is the most consumed meat in my country. In 2014, 735.1 million pigs were slaughtered in my country, and the output of pork was 56.71 million tons. However, large-scale pig raising brings a lot of pig manure, and every 100 kg pigs discharge up to 10 kg of feces and urine per day. The smell of pig manure is disgusting, and the parasite eggs exceed the standard. It is not suitable to be directly used as fertilizer, let alone be discharged at will. At present, the processing methods of pig manure include biogas fermentation method, composting fermentation method, direct returning method and so on. For example, Qin Yingli...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F15/00C05F17/00C12R1/125
CPCY02W30/40
Inventor 林元山方俊田云冯波易旭东尹翌刘博伦
Owner HUNAN AGRICULTURAL UNIV
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