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A kind of method for preparing high-purity chlorogenic acid from crude extract of Eucommia ulmoides leaves

A technology of crude extract and Eucommia ulmoides leaves, applied in the field of preparing high-purity chlorogenic acid, can solve problems such as no reports, and achieve the effects of promoting rational utilization, expanding raw material sources, and reducing losses

Active Publication Date: 2017-06-16
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] After reviewing the literature, we learned that membrane separation technology, macroporous resin separation and purification technology and activated carbon decolorization technology have all been used in the preparation of chlorogenic acid, but the combination of the three technologies has not been reported in the research of preparation of high-purity chlorogenic acid.

Method used

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  • A kind of method for preparing high-purity chlorogenic acid from crude extract of Eucommia ulmoides leaves
  • A kind of method for preparing high-purity chlorogenic acid from crude extract of Eucommia ulmoides leaves

Examples

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Effect test

Embodiment 1

[0033] Add 100g Eucommia leaves crude extract, add 500ml water to fully dissolve, stir and prepare the Eucommia leaves crude extract aqueous solution, adjust the pH of the aqueous solution to 4 with a pH meter, first pass the above aqueous solution through an ultrafiltration membrane with a molecular weight cut-off of 1000 for membrane separation, collect Permeate, pass the permeate through NKA-9 macroporous resin at a flow rate of 10BV / h to fully absorb to saturation, and rinse the above macroporous resin column with deionized water at a flow rate of 3BV / h until the effluent is colorless At this time, the NKA-9 type macroporous resin A column and the ADS-8 type macroporous resin B column are connected in series, and then 60% ethanol solution is used to pass through the A column and the B column at a flow rate of 8BV / h for elution and removal. impurity, collect the eluate obtained after impurity removal to high performance liquid chromatography (HPLC) to detect that there is no...

Embodiment 2

[0035]Add 400g of eucommia leaf crude extract, add 4000ml of deionized water to fully dissolve, stir and prepare an aqueous solution of eucommia leaf crude extract, use a pH meter to adjust the pH of the aqueous solution to 4, and pass the above aqueous solution through an ultrafiltration membrane with a molecular weight cut-off of 1000 for membrane separation , collect the permeate, pass the permeate through the ADS-7 macroporous resin at a flow rate of 8BV / h for dynamic adsorption to saturation, and rinse the above macroporous resin column with deionized water at a flow rate of 2BV / h to the effluent Colorless, at this time, connect ADS-7 type macroporous resin column and HPD-300 type macroporous resin column in series first, then use ethanol solution with a volume fraction of 50% to flow through the adsorption column ADS-7 at a flow rate of 7BV / h Type macroporous resin column and impurity removal column HPD-300 type macroporous resin column for elution and impurity removal, c...

Embodiment 3

[0037] 1000g Eucommia leaf crude extract, add 10000ml deionized water to fully dissolve, stir to prepare the Eucommia leaf crude extract aqueous solution, adjust the pH of the aqueous solution to 4 with a pH meter, and pass the above aqueous solution through an ultrafiltration membrane with a molecular weight cut-off of 1000 for membrane separation , collect the permeate, pass the permeate through the ADS-F8 macroporous resin at a flow rate of 5BV / h to absorb to saturation, rinse the above macroporous resin column with deionized water at 6BV / h until the effluent is colorless, At this time, the ADS-F8 type macroporous resin column and the BS-65 type macroporous resin are connected in series, and then the ethanol solution with a volume fraction of 60% is used to pass through the above-mentioned series of columns successively at a flow rate of 8BV / h for elution and removal of impurities. , collect the ethanol eluate and carry out membrane concentration through a nanofiltration mem...

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Abstract

The invention relates to a method for preparing high-purity chlorogenic acid, in particular to a method for preparing high-purity chlorogenic acid from the crude extract of eucommia leaves. Specifically, the crude extract of eucommia leaves is used as raw material, which is fully dissolved in deionized water to obtain an aqueous solution of the crude extract of eucommia leaves, and then separated by a membrane to collect the permeate; the permeate is adsorbed through a series of resin adsorption columns, And remove impurities, collect the eluate and concentrate, the concentrate is refined by activated carbon, and freeze-dried to obtain high-purity chlorogenic acid. The innovation of the preparation method of the present invention lies in the use of an anion-yang exchange column equipment, and the adsorption, elution and impurity removal procedures are completed in the same equipment, which saves time and operation steps. In addition, this preparation method also uses relatively large-scale equipment to process raw materials, which can process a large number of samples at one time. The process is simple and feasible, and the operation is convenient. The reagents are green and pollution-free and can be recycled. Activated carbon and macroporous resin can also be recycled and reused. , low cost, suitable for industrial production, and has good social, economic and environmental benefits.

Description

technical field [0001] The invention relates to a method for preparing high-purity chlorogenic acid, in particular to a method for preparing high-purity chlorogenic acid from Eucommia leaves crude extract, belonging to the field of natural product purification. Background technique [0002] Eucommia ulmoides Oliv. is a plant of the genus Eucommia in the Eucommia family. It is a unique economic tree species in my country, mainly distributed in Gansu, Zhejiang, Henan, Guangxi, Sichuan, Yunnan and other regions. Eucommia ulmoides is a traditional precious Chinese herbal medicine, which has the functions of curing diseases and health care without diseases. In "Shen Nong's Materia Medica", eucommia is listed as top grade. Its medicinal value has always attracted attention, and it has pharmacological effects such as invigorating the middle and nourishing qi, strengthening muscles and bones, nourishing the liver and kidney, anti-fatigue, anti-abortion, lowering blood pressure, anti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C69/732C07C67/48
CPCY02P20/582
Inventor 夏传海苗芹孙鹏程
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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