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Enzyme fertilizer and preparation method and application thereof

An enzyme fertilizer and seed technology, applied in the field of new high-efficiency compound biological fertilizers, can solve the problems of long fermentation time of materials, achieve the effects of purifying and repairing soil, reducing pollution, and inhibiting soil-borne pathogens

Active Publication Date: 2016-01-20
山东宝来利来生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the above-mentioned prior art, since no special bacterial liquid for fermentation is added in the fermentation process, the time for fermenting the material is long, and the production performance of the obtained enzyme fertilizer needs to be further improved.

Method used

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  • Enzyme fertilizer and preparation method and application thereof
  • Enzyme fertilizer and preparation method and application thereof
  • Enzyme fertilizer and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1: Screening of functional strains

[0061] 1 test material

[0062] 1.1 Strains

[0063] Three strains of lactic acid bacteria (C 2 0038、C 2 0015, C 2 0001), 3 strains of yeast (C 4 0021, C 4 0006, C 4 0030).

[0064] 1.2 Medium

[0065] MRS medium: glucose 2.0%, ammonium citrate 0.2%, sodium acetate 0.5%, dipotassium hydrogen phosphate 0.5%, manganese sulfate 0.02%, magnesium sulfate 0.05%, peptone 1.0%, beef extract 1.0%, yeast extract 0.5%, Tween-800.1%, pH6.0, all are mass ratios;

[0066] Yeast medium: 2.0% glucose, 0.5% yeast extract, 1.0% peptone, 0.2% dipotassium hydrogen phosphate, natural pH, all in mass ratio;

[0067] The reagents used above were all analytically pure.

[0068] 1.3 Reagents and formulas

[0069] 1.3.1 Reagent and formula for the determination of amylase content in enzyme fertilizer

[0070] Maltose standard solution 1mg / mL, starch standard solution 2% (mass ratio);

[0071] DNS reagent: 3.15g of 3,5-dinitrosalicylic ac...

Embodiment 2

[0148] The preparation of embodiment 2 ferment fertilizer

[0149] 1 test material

[0150]Huiyuan Juice Factory provides pomace and brown sugar with a moisture content of 5% after drying;

[0151] Lactobacillus plantarum LP fermentation broth, the number of viable bacteria is 5.23×10 8 cfu / mL;

[0152] Candida utilis CUM fermentation broth, the number of viable bacteria is 2.21×10 9 cfu / mL.

[0153] 2 test method

[0154] 2.1 Preparation method of enzyme fertilizer

[0155] In the early stage of the fermentation of the pomace, yeasts play a major role in aerobic fermentation; in the later stage, lactic acid bacteria play a major role. After mixing pomace, brown sugar, water and Candida utilis CUM fermentation broth, seal and ferment in a fermentation jar, add Lactobacillus plantarum LP fermentation broth after 30 days of fermentation, and continue to ferment for 30 days; pomace: brown sugar: water: Candida utilis CUM fermentation broth: Lactobacillus plantarum LP ferme...

Embodiment 3

[0168] Embodiment 3 has the effect verification of the enzyme fertilizer that promotes crop growth and development and improves soil effect

[0169] 1 test material

[0170] The enzyme fertilizer prepared in the embodiment of the present invention 2;

[0171] Cabbage variety: commercially available Liangfeng Four Seasons Chinese cabbage;

[0172] 2 test method

[0173] 2.1 Test grouping:

[0174] A: Blank Control group (the blank group without chemical fertilizer and fertilizer); B: CK-I (the group of single application of chemical fertilizer ammonium bicarbonate, according to 180kg / hm 2 Apply, dilute 2000 times with water during use); C: CK-II (for the enzyme fertilizer prepared by comparative example 1 of the present invention, by 180kg / hm 2 Apply, dilute 2000 times with water during use); D: CK-Ⅲ (for the enzyme fertilizer prepared by comparative example 2 of the present invention, by 180kg / hm 2 Apply, dilute 2000 times with water during use); E: EM (for the ferment fe...

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Abstract

The invention discloses application of candida utilis (CUM) and lactobacillus plantarum (LP) in preparation of enzyme fertilizer with functions of promoting crop growth and development and improving the soil effect, and further discloses the enzyme fertilizer prepared after pomace is fermented through CUM and LP, and a preparation method of the enzyme fertilizer. The method includes the following steps of firstly, mixing pomace, brown sugar, water and yeast fermentation liquor, and conducting sealed fermentation for 25-35 days; secondly, adding a lactic acid bacteria fermented solution to the material subjected to sealed fermentation, and conducting sealed fermentation for 25-35 days. The invention further discloses application of the enzyme fertilizer in crop plantation. The enzyme fertilizer contains a large amount of somatomedin, and therefore the disease resistant function of crops can be improved, pesticide residuals are greatly reduced, and growth and development of crops are promoted; soil can be purified and restored, soil-borne disease pathogen can be restrained, and resisting performance of plants is improved.

Description

technical field [0001] The invention relates to an enzyme fertilizer and its preparation method and application, and belongs to the technical field of new high-efficiency compound biological fertilizers. Background technique [0002] The irrational use of industrial fertilizers not only causes a huge waste of resources, but also causes the deterioration of natural ecology and environmental pollution. With the country's demand for ecological agriculture and sustainable agriculture, as well as the rise of global green food production and consumption, the development of green and non-toxic bio-fertilizers has become an upsurge. Biological fertilizer refers to a class of biological products rich in effective and active microorganisms, which can improve the growth environment, nutritional conditions and nutrient transformation of crops through the activities of microorganisms, and inhibit the biological activity of harmful microorganisms to exert their specific fertilizer effects...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F17/00C05G3/04C05G3/80
CPCY02W30/40
Inventor 单宝龙陈雷谷巍张建梅韩广泉王红侯红燕黄启亮乔蓬蕾陶宁谢全喜于佳民侯楠楠王珂徐辉王春凤
Owner 山东宝来利来生物工程股份有限公司
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