A method for modifying the surface of collagen polypeptide nanospheres with positive charges

A collagen polypeptide and modification method technology, applied in chemical instruments and methods, specific peptides, microsphere preparation, etc., can solve the problems affecting uranium separation and recovery, difficult treatment of chemical sludge, large chemical toxicity, etc. Application prospects, the effect of simple production process

Inactive Publication Date: 2018-01-12
SOUTHWEAT UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Wastewater generated during the wet uranium production process contains relatively high concentrations of fluorine (F - ), which has high chemical toxicity and strong corrosiveness, has a strong corrosive effect on separation carriers such as ion exchange resins, and seriously affects uranium (uranyl ion UO 2 2+ ) separation and recovery
At present, precipitation method and adsorption method are considered to be the common methods to remove fluorine in water. The precipitation method has the advantages of simple operation and low cost, but the chemical sludge is difficult to deal with and has many defects.

Method used

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  • A method for modifying the surface of collagen polypeptide nanospheres with positive charges

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Step 1. Disperse 10.0 mg of collagen peptide nanospheres in 150 mL of distilled water, and add 0.5 mg of Al while stirring 2 (SO 4 ) 3 , stirred the reaction for 2 hours, and then used 1mol.L -1 NaHCO 3 The pH value of the solution was adjusted to 4.5, and the stirring reaction was continued for 30 minutes, centrifuged and separated to obtain Al 3+ Modified collagen peptide nanospheres;

[0031] Step two, take 10mgAl 3+ The modified collagen polypeptide nanospheres were dispersed in 150mL of distilled water, and 1mg of dodecyltrimethylammonium chloride was added while stirring, and 1mol.L -1 Adjust the pH value of the system to 5 with HCl, protect it with nitrogen (purity ≥ 99.99%), add 0.3 mg of azobisisobutyronitrile, stir and react in a water bath at 25°C for 7 hours, centrifuge, separate, and freeze-dry to obtain positively charged surface-modified collagen Peptide Nanospheres.

Embodiment 2

[0033] Step 1. Disperse 10.0 mg of collagen peptide nanospheres in 150 mL of distilled water, and add 0.3 mg of Al while stirring 2 (SO 4 ) 3 , stirred the reaction for 3 hours, and then used 1mol.L -1 NaHCO 3 The pH value of the solution was adjusted to 4.5, and the stirring reaction was continued for 30 minutes, centrifuged and separated to obtain Al 3+ Modified collagen peptide nanospheres;

[0034] Step two, take 10mgAl 3+ The modified collagen polypeptide nanospheres were dispersed in 150mL of distilled water, and 1mg of methacryloyloxyethyltrimethylammonium chloride was added while stirring, and mixed with 1mol.L -1 Adjust the pH value of the system to 5 with HCl, pass nitrogen (purity ≥ 99.99%) for protection, add 0.3 mg of dibenzoyl peroxide, stir and react in a water bath at 25 ° C for 7 hours, centrifuge, separate, and freeze-dry to obtain positively charged surface-modified collagen Peptide Nanospheres.

Embodiment 3

[0036] Step 1. Disperse 10.0 mg of collagen peptide nanospheres in 150 mL of distilled water, and add 0.3 mg of Al while stirring 2 (SO 4 ) 3 , stirred the reaction for 3 hours, and then used 1mol.L -1NaHCO 3 The pH value of the solution was adjusted to 4.5, and the stirring reaction was continued for 30 minutes, centrifuged and separated to obtain Al 3+ Modified collagen peptide nanospheres;

[0037] Step two, take 10mgAl 3+ The modified collagen polypeptide nanospheres were dispersed in 150mL of distilled water, and 1.5mg of dodecyldimethylbenzyl ammonium chloride was added while stirring, and mixed with 1mol.L -1 Adjust the pH value of the system to 5 with HCl, pass nitrogen (purity ≥ 99.99%) for protection, add 0.5 mg of ammonium persulfate, stir and react in a water bath at 25°C for 10 hours, centrifuge, separate, and freeze-dry to obtain positively charged surface-modified collagen polypeptide nanospheres .

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Abstract

The invention discloses a method for modifying the positive charge on the surface of collagen polypeptide nanospheres. The method comprises: taking collagen polypeptide nanospheres and dispersing them in distilled water, adding Al2(SO4)3 while stirring, stirring and reacting, and then adjusting the pH value of the system to 3.5 ~5.0, continue to stir the reaction, centrifuge, and separate to obtain Al3+ modified collagen polypeptide nanospheres; take Al3+ modified collagen polypeptide nanospheres and disperse them in distilled water. Nitrogen protection, adding an initiator, stirring for reaction, centrifugation, separation, and freeze-drying to obtain positively charged surface-modified collagen polypeptide nanospheres. According to the principle of aluminum tanning in the chemical method of tanning, the present invention introduces cations with good binding ability to fluoride ions through the reaction of Al3+ and collagen polypeptide nanospheres, and further introduces strong electropositive compounds through Michael addition reaction, and combines them with nanoparticles Excellent performance, can effectively adsorb and separate fluoride ions in wastewater.

Description

technical field [0001] The invention relates to a method for modifying the positive charge on the surface of collagen polypeptide nanospheres, and belongs to the technical field of tanning waste-collagen reuse. Background technique [0002] Wastewater generated during the wet uranium production process contains relatively high concentrations of fluorine (F - ), which has high chemical toxicity and strong corrosiveness, has a strong corrosive effect on separation carriers such as ion exchange resins, and seriously affects uranium (uranyl ion UO 2 2+ ) for separation and recovery. At present, precipitation and adsorption are considered to be the common methods to remove fluorine in water. The precipitation method has the advantages of simple operation and low cost, but the chemical sludge is difficult to deal with and has many defects. The commonly used adsorption method is an effective method to treat low-concentration fluorine-containing wastewater. Commonly used adsorben...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J13/02B01J20/26B01J20/30C07K14/78C07K1/12
Inventor 周建余杰苏林罗学刚
Owner SOUTHWEAT UNIV OF SCI & TECH
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