Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing particle-size-controllable type starch nanoparticles by emulsifier assistant biological enzyme method

A starch nanometer and biological enzyme method technology, applied in the nanometer field, can solve the problems of large particle size, poor dispersion, poor stability and uneven size distribution of nanoparticles, achieve low equipment requirements, good dispersibility and thermal stability, Crystallize the perfect effect

Active Publication Date: 2016-01-06
QINGDAO AGRI UNIV
View PDF2 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the disadvantages of large particle size, uneven size distribution, poor dispersion, and poor stability in the prior art, the purpose of the present invention is to provide an emulsifier-assisted biological enzyme method to prepare particle size-controllable starch nanoparticles The method uses an emulsifier to control the recrystallization and crystal nucleus growth rate of short amylose, and controls the further aggregation of nanoparticles. The particle size distribution of the prepared starch nanoparticles is 9-30nm

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing particle-size-controllable type starch nanoparticles by emulsifier assistant biological enzyme method
  • Method for preparing particle-size-controllable type starch nanoparticles by emulsifier assistant biological enzyme method
  • Method for preparing particle-size-controllable type starch nanoparticles by emulsifier assistant biological enzyme method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052](1) Preparation of buffer solution: Accurately weigh 3 g of disodium hydrogen phosphate and 2 g of citric acid and dissolve in 200 mL of distilled water, stir to fully dissolve, and set aside;

[0053] (2) Activation of debranching enzyme: Accurately weigh 1.1 mL of pullulanase and drop it into 50 mL of distilled water, stir to make it fully mixed, and set aside;

[0054] (3) Preparation of starch milk: Weigh 15g waxy cornstarch and add in 100mL buffer solution to obtain 15% starch emulsion;

[0055] (4) Gelatinization: bath the prepared starch milk at 100°C for 20 minutes to completely gelatinize the starch, then cool down to 60°C;

[0056] (5) Enzymatic hydrolysis and debranching: add the treated pullulanase to the gelatinized colloidal solution, and enzymatic hydrolysis for 4 hours;

[0057] (6) Separation of short amylose solution: centrifuge the obtained solution for 3 min at a speed of 3000 r / min while hot to remove long amylose to obtain a short amylose solution;...

Embodiment 2

[0063] (1) Preparation of buffer solution: Accurately weigh 3.5 g of disodium hydrogen phosphate and 2.3 g of citric acid and dissolve them in 200 mL of distilled water, stir to fully dissolve, and set aside;

[0064] (2) Activation of debranching enzyme: Accurately weigh 1.5 mL of pullulanase and drop it into 50 mL of distilled water, stir to make it fully mixed, and set aside;

[0065] (3) Preparation of starch milk: Weigh 10 g of waxy rice starch and add it to 100 mL of buffer solution to obtain 10% starch emulsion;

[0066] (4) Gelatinization: put the prepared starch milk in a water bath at 100°C for 30 minutes to completely gelatinize the starch, and then lower the temperature to 58°C (the optimum temperature for pullulanase);

[0067] (5) Enzymatic hydrolysis and debranching: add the treated pullulanase to the gelatinized colloidal solution, and enzymatic hydrolysis for 6 hours;

[0068] (6) Separation of short amylose solution: centrifuge the obtained solution for 3 mi...

Embodiment 3

[0074] (1) Preparation of buffer solution: Accurately weigh 3.7g of disodium hydrogen phosphate and 2.0g of citric acid and dissolve in 200mL of distilled water, stir to fully dissolve, and set aside;

[0075] (2) Activation of debranching enzyme: Accurately weigh 1 mL of pullulanase and drop it into 50 mL of distilled water, stir to make it fully mixed, and set aside;

[0076] (3) Preparation of starch milk: take 20g of taro starch and add it to 100mL buffer solution to obtain 20% starch emulsion;

[0077] (4) Gelatinization: put the prepared starch milk in a water bath at 100°C for 40 minutes to completely gelatinize the starch, and then lower the temperature to 50°C (the optimum temperature for pullulanase);

[0078] (5) Enzymatic hydrolysis and debranching: add the treated pullulanase to the gelatinized colloidal solution, and enzymatic hydrolysis for 5 hours;

[0079] (6) Separation of short amylose solution: centrifuge the solution obtained while hot at a speed of 3000r...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing particle-size-controllable type starch nanoparticles by an emulsifier assistant biological enzyme method, and is characterized in that the method specifically comprises the following steps: (1) preparation of a buffer solution; (2) debranching enzyme activation; (3) preparation of starch milk; (4) gelatinization (starch chain extension); (5) enzymolysis debranching; (6) short straight-chain solution separation; (7) enzyme deactivation; (8) addition of an emulsifier, and control of a crystallization process; (9) recrystallization; and (10) drying. The method disclosed by the invention has the advantages of low equipment requirement, simple technology, simple and convenient operation, moderate reaction, short reaction time, and high efficiency, and is suitable for large-scale production; and the product performance is good, the particle diameter of the prepared starch nanoparticles can be controlled to be 9-30 nm, the dispersion and thermal stability are good, the preparation cost is low, the energy consumption is low, no harmful waste is generated, and the method conforms to modern production requirements of environmentally-friendly production and energy conservation.

Description

technical field [0001] The invention relates to the field of nanotechnology, in particular to a method for preparing starch nanoparticles with a controllable particle size by an emulsifier-assisted biological enzyme method. Background technique [0002] Starch nanoparticles are a kind of nano-sized polysaccharide crystals prepared from native starch, which exhibit many physical and chemical properties due to properties such as small size effect, surface effect, and quantum size effect. Since the last century, nanotechnology has attracted sufficient attention and attention from all walks of life. So far, starch nanoparticles made of organic polymers such as inorganic substances and natural polymers, semi-synthetic polymers and synthetic polymers have been used as raw materials. It is widely used in many fields such as biology, chemical industry, medicine and food. Among them, starch nanoparticles are suitable for many fields including the pharmaceutical field due to their wi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08J3/12C08L3/12C08B30/20
Inventor 孙庆杰熊柳李晓静秦洋刘成珍
Owner QINGDAO AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com