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Lyophyllum decastes strain Finc-L6 and cultivation method and application thereof

A lotus-leaf Umbrella, finc-l6 technology, applied to the lotus-leaf Umbrella Finc-L6 strain and its cultivation field, can solve the problems of difficulty in selective breeding, prolong the culture period, etc. The effect of short cultivation period and lower production cost

Active Publication Date: 2016-01-06
青岛丰科生物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, the new varieties of Heyelizhi mushroom are mainly obtained by wild domestication, that is, selective breeding. However, due to excessive picking, wild Heyeli mushrooms are very few, and selective breeding is becoming more and more difficult.
However, the yield per unit area of ​​the current lotus-leaf-shimella varieties is still low. In addition, if you want to obtain a longer fresh-keeping period, you need to greatly extend the cultivation period, which is a major defect for industrial production and market supply.

Method used

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  • Lyophyllum decastes strain Finc-L6 and cultivation method and application thereof
  • Lyophyllum decastes strain Finc-L6 and cultivation method and application thereof
  • Lyophyllum decastes strain Finc-L6 and cultivation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1 hybridization obtains Finc-L6

[0055] Finc-L6 was obtained by cross breeding method.

[0056] Specific steps are as follows:

[0057] 1) Select parental strains: select the strains Finc-LD-2 and Finc-LD-3 domesticated by Shanghai Fengke Biotechnology Co., Ltd. as parents;

[0058] 2) Prepare mother seed culture medium, original seed culture medium and cultivar culture medium: the formulations of each culture medium have been given, and will not be repeated here;

[0059] 3) Isolation of basidiospores: place the caps of Finc-LD-2 and Finc-LD-3 in a petri dish respectively, after 24 hours, collect the spores falling on the petri dish, and dilute them into an appropriate amount in sterile water. Concentration, and spread on the PDA medium plate. Cultivate at 23°C for 25 days, and inoculate PDA slant test tubes with inoculation loops after the spores germinate. These tubes were incubated at 23°C for 25 days, after which hyphae were isolated from the tubes ...

Embodiment 2

[0063] Embodiment 2 Antagonism experiment

[0064] Finc-L6 of the present invention, 2 parent strains Finc-LD-2, Finc-LD-3 and 2 control strains Finc-LD-3, Finc-LD-4 are test materials, and the medium used is PDA medium.

[0065] Finc-L6, Finc-LD-1, Finc-LD-2, Finc-LD-3, and Finc-LD-4 were inoculated and cultured on the plate medium respectively. The distance between the inoculation points was 2.5 cm, and they were cultured at 23°C for 25 days. , see Table 1 for the test results.

[0066] Table 1 Antagonism test among different strains

[0067] Variety Finc-LD-1 Finc-LD-2 Finc-LD-3 Finc-LD-4 Finc-L6 antagonistic antagonistic antagonistic antagonistic

[0068] Such as Figure 1-8 As shown, there are obvious antagonistic lines between Finc-L6 and Finc-LD-1, Finc-LD-2, Finc-LD-3, and Finc-LD-4 at the junction of confrontation culture colonies, indicating that Finc-L6 is related to Finc-LD-1, Finc-LD-2, Finc-LD-3, and Finc-LD-4 are different ...

Embodiment 3

[0069] Embodiment 3 cultivation experiment

[0070] The lotus-leaved mushroom strain Finc-L6 of the present invention, parent strains Finc-LD-2 and Finc-LD-3, and existing varieties Finc-LD-1, Finc-LD-4 are cultivated and cultivated respectively, each 500 bottles of the bacterial strain were cultivated, and then the results of small batch cultivation were summarized and compared, and the advantages and characteristics of the bacterial strain Finc-L6 of the present invention were summarized.

[0071] The culture medium of this embodiment participates in the cultivar culture medium, and will not be repeated here.

[0072] Cultivation bottle volume: 850cc, bottle mouth diameter 58mm.

[0073] Cultivation: temperature 23~28℃, air relative humidity 75%, carbon dioxide concentration 2000~3500ppm;

[0074] Cultivation: The fruiting temperature is set at 15°C-18°C, the relative air humidity is 93%-100%, the carbon dioxide concentration is 1000-2000ppm, the light intensity is 10-50Lu...

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Abstract

The invention belongs to the technical field of edible fungi and particularly relates to a lyophyllum decastes strain Finc-L6 and a cultivation method and application thereof. The lyophyllum decastes strain is named Finc-L6, and obtained in the mode that a strain Finc-LD-2 and a strain Finc-LD-3 serve as parents to carry out hybridization, and systemic breeding is carried out, the lyophyllum decastes strain belongs to Lyophyllum decastes and is preserved in the common microorganism center of the China microorganism strain preservation management council, the address of the center is room 3, Beichen west road No.3, Chaoyang district of Beijing, China, the preservation serial number is CGMCC No.11201, and the preservation date is August 13th, 2015. According to the technical scheme, the blank of lyophyllum decastes hybridization breeding is filled up, and the obtained novel strain is short in cultivation period, high in per unit yield, beautiful in flower shape and suitable for large-area plantation and popularization, fruiting time is centralized, the freshness keeping period is long, and the market prospect is wide.

Description

technical field [0001] The invention belongs to the technical field of edible fungi, and in particular relates to the Finc-L6 bacterial strain of the mushroom of the lotus leaf and its cultivation method and application. Background technique [0002] Lotus leaf from the mushroom (Latin name Lyophyllumdecastes), belonging to Basidiomycotina (Basidiomycotina), layer fungi (Hymeno-myceyes), Agaricales (Agaricales), white mushroom (Tricholomataceae), from the genus Lyophyllum ), is an excellent edible fungus that has been researched and cultivated by the edible fungi industry both at home and abroad. The flesh of lotus leaf phyllomycetes is thick and delicate, fragrant and tangy, crisp and smooth, delicious, rich in nutrition, and has shown great advantages in the repair of human cells and in the treatment of cardiovascular diseases and anti-aging. It is a rare edible fungus that can be used both as food and medicine. Its food and medicine value has been accepted and recognized...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
CPCA01G18/00
Inventor 韩成玲吴慧敏张文宏
Owner 青岛丰科生物科技有限公司
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