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Nanoprobe for heavy metal ion up-conversion luminescence detection and preparation method thereof

A technology for heavy metal ion and luminescence detection, applied in the direction of material excitation analysis, can solve the problems of inability to analyze multiple elements at the same time, limited application elements, large linear range, etc., and achieves the effect of avoiding light damage, mild experimental conditions and good morphology.

Inactive Publication Date: 2015-12-30
SHANGHAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the widely used methods for detecting heavy metal ions are: atomic absorption spectrometry (AAS), atomic fluorescence spectrometry (AFS), inductively coupled plasma mass spectrometry (ICP-MS), etc. Also very mature, but every technology has limitations
Atomic absorption spectrometry has the advantages of good selectivity, high sensitivity, fast analysis speed, and strong anti-interference ability. However, the determination of each element requires a corresponding hollow cathode lamp, and simultaneous analysis of multiple elements is not possible.
The detection limit of atomic fluorescence spectrometry is lower than that of atomic absorption method, the spectral line is clear, less interference, high sensitivity, large linear range, but the application elements are limited
Inductively coupled plasma mass spectrometry has lower detection limits than atomic absorption and is the state-of-the-art method in the field of trace element analysis, but is expensive and prone to contamination

Method used

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  • Nanoprobe for heavy metal ion up-conversion luminescence detection and preparation method thereof
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  • Nanoprobe for heavy metal ion up-conversion luminescence detection and preparation method thereof

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preparation example Construction

[0027] For the preparation method of up-conversion luminescent nanocrystals with core-shell structure, please refer to the literature:

[0028] Li, Z.; Zhang, Y., A efficient and user-friendly method for the synthesis of hexagonal-phase NaYF 4 :Yb,Er / Tmnanocrystalswithcontrollableshapeandupconversionfluorescence. Nanotechnology 2008 , 19 (34), 345606.

Embodiment 1

[0030] This embodiment provides an example of a synthetic method for the nanoprobe Cit-UCNPs-ssDNA1-SWCNHs for upconversion luminescence detection of heavy metal lead ions, which includes the following steps:

[0031] 1. Preparation of Cit-UCNPs nanocrystals:

[0032] 1. Disperse 8~10mg of CS-UCNPs in the prepared 5mL mixed solution of toluene and chloroform, wherein the volume ratio of toluene and chloroform is 1:2~2:3;

[0033] 2. Under an inert atmosphere, dissolve 0.4~0.6g of anhydrous sodium citrate in 15mL of diethylene glycol, heat to 110°C, keep for 30~40min, and cool;

[0034] 3. Slowly add the nanocrystals obtained in step 1 to the solution obtained in step 2, heat to 130°C, keep for 40~50min, evaporate toluene and chloroform, heat to 180°C, and keep for 1 ~2 hours, cooled, centrifuged, and washed with ethanol and water to obtain Cit-UCNPs nanocrystals.

[0035] 2. Preparation of energy donor, namely Cit-UCNPs-ssDNA1

[0036]1. Prepare nanocrystalline Cit-UCNPs 3~...

Embodiment 2

[0043] This embodiment provides an example of a synthetic method for the nanoprobe Cit-UCNPs-ssDNA2-GO used for upconversion luminescence detection of heavy metal mercury ions, which includes the following steps:

[0044] 1. Preparation of energy donor, namely Cit-UCNPs-ssDNA2

[0045] 1. Prepare 3~4mg of Cit-UCNPs, 3~4mg of N-hydroxysuccinimide (NHS), 2~3mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC), 100μMHg 2+ Probe DNA2 molecule 20~30μL, deionized water 1~3mL; the sequence of probe DNA2 is: 5'-NH 2 -TTCTTCTTTCTTCCCCTTGTTTGTTG-3', the probe DNA is DNA that can specifically recognize heavy metal mercury ions.

[0046] 2. Add the prepared NHS and EDC into 1~3mL deionized water according to the mass ratio of 1:1~3:1, then add the prepared Cit-UCNPs nanocrystals, stir and react at 4°C for 2~4 hours, and the obtained The mixture was centrifuged and washed 3 times with deionized water to obtain activated Cit-UCNPs nanocrystals;

[0047] 2. Dissolve the activated Ci...

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Abstract

The invention relates to a nanoprobe for heavy metal ion up-conversion luminescence detection and a preparation method thereof. The nanoprobe is formed by combining an energy donor and an energy receptor through pi-pi bond interaction and is characterized in that the energy donor is formed by carboxyl-functionalized up-conversion luminescence nanocrystallines (CS-UCNPs) with a core-shell structure and probe DNA molecules capable of specifically identifying heavy metal ions through covalent bond assembly, wherein the molar ratio of probe DNA to the up-conversion luminescence nanocrystallines is 1:2000-2500. The surface carboxylation rate of the up-conversion luminescence nanocrystallines is 30%-60%. The energy receptor is single-walled carbon nanohorns (SWCNHs), graphene oxide (GO) or carbon nanotubes (WCNTs). The mass ratio of the energy donor to the energy receptor is 15:1-30:1. The preparation method has the advantages of being simple in process, convenient to operate and easy to control structurally, the prepared nanoprobe is uniform in size and stable in structure, has the advantages of being low in toxicity and good in biocompatibility and has potential application value in the fields of cell genetics, research on molecular biology and the like.

Description

technical field [0001] The invention relates to a nanoprobe for upconversion luminescence detection of heavy metal ions and a preparation method thereof. Background technique [0002] Heavy metal pollution has become one of the most serious environmental and ecological pollution. Because heavy metals are not easy to remove after being discharged into the environment, they accumulate in the environment for a long time, which will pose a threat to the survival of organisms and human health. The way heavy metals harm the human body is mainly by changing the structure of enzymes. Some heavy metal ions will interfere with the metabolism of essential metal ions in the human body, and inactivate enzymes by replacing other essential metal ions in enzymes. For example, Hg 2+ Highly toxic, it enters the human body through the food chain and gradually accumulates. It can combine with sulfhydryl groups of various proteins, destroy cell metabolism, liver detoxification function, and cau...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/63
Inventor 施利毅刘金亮徐艳霞孙丽宁孟宪福
Owner SHANGHAI UNIV
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