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Bacillus amyloliquefaciens subsp. plantarum strain for producing cellulase

A technology of amylolytic spores and bacillus plants, applied in the field of microorganisms, can solve the problem of low enzyme activity, achieve the effect of good enzyme activity and broaden the source

Inactive Publication Date: 2015-12-23
福建省农业科学院农业生物资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few literature reports on Bacillus amyloliquefaciens producing cellulase, and the enzyme activity is very low

Method used

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  • Bacillus amyloliquefaciens subsp. plantarum strain for producing cellulase
  • Bacillus amyloliquefaciens subsp. plantarum strain for producing cellulase
  • Bacillus amyloliquefaciens subsp. plantarum strain for producing cellulase

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] The isolation and screening of the bacterial strain FJAT-29941 of embodiment 1

[0013] (1) Collection of samples: Take the plant roselle root soil from the experimental base of Sugarcane Research Institute in Zhangzhou City, Fujian Province, put it into a sterile collection bag, store it at 4°C, and store it for later use;

[0014] (2) Isolation of bacterial strains: take 10g of soil in 90mL sterile water, draw 1mL for gradient dilution after fully shaking, and choose a dilution of 10 -1 , 10 -2 , 10 -3 ; Then spread the soil dilution on the bacterial culture medium plate and culture at 15°C for 3-5 days; then inoculate the colonies obtained from the culture on the bacterial medium plate by continuous streaking method, and purify and culture at 15°C 72h, obtain pure bacterial strain;

[0015] (3) Screening of bacterial strains: Inoculate the isolated pure bacterial strains in cellulose sodium liquid medium (CMC-Na) by spot-patch method, culture at 30°C for 48 hours,...

Embodiment 2

[0017] Identification of the bacterial strain FJAT-29941 of embodiment 2

[0018] (1) Morphological identification:

[0019] The main morphology of the strain FJAT-29941 was identified, and the main morphology of the strain FJAT-29941 was obtained as follows: the color of the colony is off-white, the colony is round, the surface is dry, the edges are irregular, opaque, and Gram staining is positive.

[0020] (2) Identification of physiological and biochemical characteristics:

[0021] The physiological and biochemical characteristics of the strain FJAT-29941 were identified using the physiological and biochemical micro-identification tubes provided by Beijing Luqiao Company. The identification results are shown in Table 1 below.

[0022] Table 1 Physiological and biochemical identification results of strain FJAT-29941

[0023]

[0024] Note: In Table 1, "+" means there is an effect or a response; "-" means no effect or no response.

[0025] (3) Determination and analysis...

Embodiment 3

[0032] Example 3 Determination of cellulase activity produced by the bacterial strain FJAT-29941

[0033] (1) Drawing of glucose standard curve:

[0034] a. Glucose is dried overnight in an oven at 80°C;

[0035] b. Preparation of glucose solution: take 100 mg of glucose dried to constant weight, dissolve it in sterile water and dilute it to 100 mL to obtain a standard glucose solution (concentration is 1.00 mg / ml), and place it in the refrigerator for subsequent use;

[0036] c. Determination of the standard curve: take 7 test tubes with 20mL scale, and perform operations according to Table 2. Scan the full spectrum with a microplate reader, and draw a tyrosine standard curve based on the data (see figure 2 ).

[0037] Table 2 glucose standard curve

[0038]

[0039]

[0040] (2) Determination of enzyme activity:

[0041] The strain FJAT-29941 was inoculated into CMC-Na liquid medium with 1% inoculation amount, cultured in a shaker at 170 rpm and 30°C for 48 hours...

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Abstract

The invention provides a bacillus amyloliquefaciens subsp. plantarum strain FJAT-29941 for producing cellulase. The bacillus amyloliquefaciens subsp. plantarum strain FJAT-29941 is preserved in China General Microbiological Culture Collection Center, which is located at No.3, No.1 courtyard, Beichen West Road, Chaoyang District, Beijing, on Feburary 10th, 2015, and the preservation number of the bacillus amyloliquefaciens subsp. plantarum strain FJAT-29941 is CGMCC No. 10558. The cellulase produced by the bacillus amyloliquefaciens subsp. plantarum strain FJAT-29941 is good in enzyme activity, the source of good cellulase is expanded, and possibility of more cellulase application is provided.

Description

【Technical field】 [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a cellulase-producing Bacillus amyloliquefaciens plant subspecies strain. 【Background technique】 [0002] Cellulose is one of the cheapest and most abundant renewable resources on earth. However, due to the particularity of its molecular structure and its existence in nature, cellulose resources have not been fully developed and utilized reasonably, and incineration or disposal will cause environmental pollution. The mechanism of cellulase-producing cellulolytic bacteria degrading fiber is through a series of actions such as attachment, adhesion, and penetration with crude fiber, producing cellulase to decompose the components of fiber, and cellulose is gradually converted into glucose Or single-cell protein, etc., and further converted into alcohol, etc., can not reduce environmental pollution, or can be efficiently used in energy, feed, food, textile, pape...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/42C12R1/07
Inventor 刘波刘国红车建美朱育菁陈峥陈倩倩唐建阳
Owner 福建省农业科学院农业生物资源研究所
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