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Application of Chinese herbal medicament to delaying of bacterial drug resistance

A technology of Chinese herbal medicine and drug resistance, applied in the direction of antibacterial drugs, medical preparations containing active ingredients, pharmaceutical formulas, etc., to achieve the effect of delaying drug resistance

Inactive Publication Date: 2015-12-23
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no report about the application of the Chinese herbal medicine of the present invention in delaying bacterial drug resistance

Method used

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  • Application of Chinese herbal medicament to delaying of bacterial drug resistance
  • Application of Chinese herbal medicament to delaying of bacterial drug resistance
  • Application of Chinese herbal medicament to delaying of bacterial drug resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 screening has the Chinese herbal medicine that delays drug resistance

[0023] The main work includes: exploring the delaying effect of Chinese herbal medicines on Aeromonas or Vibrio drug resistance, and screening Chinese herbal medicines that have the effect of delaying drug resistance

[0024] 1 Screening of drug-resistant strains

[0025] The Aeromonas hydrophila standard strain ATCC7966 (hereinafter referred to as SS) was selected as the target strain, and the drug resistance of the Aeromonas hydrophila standard strain ATCC7966 was screened, and five antibiotics (enrofloxacin, fluorobenzene, etc.) were initially selected. Nicotine, doxycycline hydrochloride, compound metamethoxan sodium and neomycin sulfate), and strains with strong resistance to these five antibiotics were screened out as further experimental objects.

[0026] 1.1 Determination of the minimum inhibitory concentration (MIC) of five antibiotics on SS

[0027] The MIC of five kinds of ...

Embodiment 2

[0057] Example 2 Analysis of the drug resistance mechanism of Aeromonas hydrophila

[0058] Transcriptome analysis of the mechanism of enrofloxacin resistance in Aeromonas hydrophila

[0059] 1 experimental strain

[0060] There are two experimental strains for transcriptome sequencing, Aeromonas hydrophila ATCC7966(SS) with an MIC of 0.25 μg / mL and the drug-resistant Aeromonas hydrophila with an MIC of 64 μg / mL that has been induced before Bacteria ATCC7966-RS (RS).

[0061] 2 Partial data analysis of transcriptome sequencing

[0062] After extraction of sample RNA, sequencing analysis was performed. After assembling and splicing the raw data after sequencing, 2353, 1821, and 4151 unigenes (non-repeated sequence genes) belonging to RS, SS, and RS&SS were obtained, respectively. Among the 4151 unigenes in RS&SS, 3237 unigenes were annotated by Swiss-Protdatabase, and 3944 unigenes were annotated by NCBI protein database. Through BLAST and BLAST2GO software analysis, 2548 ...

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Abstract

The invention relates to application of Chinese herbal medicament to delaying of bacterial drug resistance. The Chinese herbal medicament comprises isatis roots, blackberry lily, radix sophorae flavescentis, folium isatidis, plantain herbs, fructus forsythiae, scutellaria baicalensis or wormwood. The Chinese herbal medicament has the advantage that the traditional Chinese medicine which is selected out has the effect of delaying aeromonas drug resistance.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of Chinese herbal medicine in delaying bacterial drug resistance. Background technique [0002] Certain substances are known to kill microbes, but there are also some microbes that survive normal lethal doses. In 1907, Ehrlich studied the formation of resistance to p-roseaniline in Trypanosoma brucei; in 1911, Morgenroth and Kaufmann reported the resistance of Streptococcus pneumoniae to ethanoquinine. Every antibacterial drug can kill or inhibit microorganisms, but at the same time, its resistance also emerges. [0003] In recent years, due to the wide application of antibiotics and other chemical antibacterial drugs, drug-resistant bacteria have grown rapidly, making effective treatment of infectious diseases a serious problem that plagues human and animal health. Therefore, research on how to delay bacterial resistance has become a hot spot of concern. [0004] ...

Claims

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Application Information

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IPC IPC(8): A61K36/88A61P31/04
Inventor 董亚萍
Owner SHANGHAI OCEAN UNIV
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