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Method for identifying resistance of soybeans to soybean phytophthora blight and application of method in breeding

A technology of blight resistance and Phytophthora sojae, applied in soybean breeding, identification of soybean resistance to soybean blight, can solve problems such as loss of F2 population of heterozygous disease-resistant plants, and avoid population loss and time-consuming short, small footprint

Inactive Publication Date: 2015-12-23
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to improve the identification efficiency of soybean resistance to soybean blight, and to avoid the defect that traditional identification methods will cause the loss of F2 population of heterozygous disease-resistant plants, and to provide a new method for identifying soybean resistance to soybean blight and its application in soybean breeding

Method used

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  • Method for identifying resistance of soybeans to soybean phytophthora blight and application of method in breeding
  • Method for identifying resistance of soybeans to soybean phytophthora blight and application of method in breeding
  • Method for identifying resistance of soybeans to soybean phytophthora blight and application of method in breeding

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Embodiment 1

[0035] Embodiment 1 A kind of method for identification soybean to soybean blight resistance

[0036] (1) Preparation of Phytophthora soybean inoculum

[0037] Phytophthora soybean strain ( 52696 TM ) were stored on the CA medium, and during the experiment, the bacterial strain was transferred to a petri dish containing 15ml of 1.5% agar-based CA medium, and cultivated in the dark at 25°C for 7-10 days, and the relative humidity of the culture environment was 90-100%. Inoculate the susceptible variety Williams (this variety is disclosed in "GeneticAnalysisofSoybeanPlantIntroductionswithResistancetoPhytophthorasojae, GordonSG, etal. Phytopathology, 2007Jan; 97(1): 106-12", it is a conventional soybean susceptible variety) to restore virulence to the seedlings, and carry out pathogenic bacteria after the onset of the plants. isolation and purification for inoculation. One week before the inoculation, the bacterial strain was transferred into a petri dish containing 15 ml of ...

Embodiment 2

[0045] Embodiment 2 A kind of method for identification soybean to soybean blight resistance

[0046] (1) Preparation of Phytophthora soybean inoculum

[0047] Phytophthora sojae strains are preserved on the carrot juice agar (CA) medium. During the experiment, the strains are transferred to the CA medium petri dish containing 15ml of 1.5% agar, cultivated in the dark at 22°C for 7-10 days, and inoculated with the susceptible variety Williams The seedlings recovered their virulence, and the pathogenic bacteria were isolated and purified after the onset of the plants, and used for inoculation. One week before inoculation, the strains were transferred to a petri dish containing 15 ml of CA medium containing 1.5% agar, and cultivated in the dark at 27°C for 7-10 days. When inoculating, use a Joyoung Soymilk machine to crush the culture medium containing mycelium to prepare inoculum, dispense it into 5ml syringes, and prepare for inoculation.

[0048] (2) Inoculate the soybean p...

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Abstract

The invention provides a method for identifying the resistance of soybeans to soybean phytophthora blight and the application of the method in breeding. The method comprises the steps of (1) inoculant preparation: inoculating a phytophthora sojae strain to a CA culture medium, a V8 culture medium or a lima bean sulture medium, enabling the phytophthora sojae strain to rejuvenate through dark culture, and processing and smashing a culture medium which is rich in thalli to obtain an inoculant; (2) soybean plant material inoculation: containing the inoculant obtained in step (1) in a detection container, selecting a green shanked leaf, which just stretches, of a to-be-detected soybean plant, and inserting a leafstalk in the inoculant in the detection container; (3) dark culture: judging a result after carrying out the dark culture by putting the detection container in which the soybean material is inoculated in step (2) in a humid confined space. The method for identifying the resistance of the soybeans on the soybean phytophthora blight, disclosed by the invention, has the advantages that the operation is simple and convenient, the cost is low, and the time is saved, repeated experiment can be carried out; meanwhile, a to-be-identified experiment material cannot be lost, and the defect in a traditional method that experiment materials are collectively lost can be avoided.

Description

technical field [0001] The invention belongs to the technical field of disease-resistant breeding, and in particular relates to a method for identifying soybean blight resistance and its application in soybean breeding. Background technique [0002] Soybean blight caused by Phytophthorasojae Kaufmann & Gerdemann is a devastating soil-borne disease in soybean production. The disease has occurred in many provinces of our country. Phytophthora sojae can infect soybeans in all growth stages of soybeans, causing root rot, stem rot, plant dwarfing, withering and death. Generally, the yield of fields is reduced by 10%-30%, and in severe fields it can reach 60%-90%. even lead to miscarriage. The current worldwide economic loss due to soybean blight is approximately US$ 1 billion per year (Tyler, 2007). [0003] At present, researchers generally identify the resistance of soybean to soybean blight through hypocotyl wound inoculation. The hypocotyl inoculation method can only be c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H3/00A01H3/04
Inventor 朱振东
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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