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A kind of preparation method of salicornia rutin algastatic agent

A technology of salt hornwort and algae inhibitor, which is applied in the field of preparation of salt hornwort rutin algae inhibitor, can solve the problems of easy secondary outbreak, insufficient treatment, and insignificant effect, and achieve no secondary pollution, green The effect of environmental protection and secondary pollution

Active Publication Date: 2017-11-28
上海双骏环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem mainly solved by the present invention: for the eutrophication of water body, the treatment of algae is relatively complicated, and the treatment of algae in water body by ordinary physical methods is not thorough enough and easy to cause secondary outbreaks, while the treatment by biological method can inhibit the growth of algae for a long time and the effect is not significant To solve the problem, we provide a method for preparing algae inhibitor by cultivating Salicornia, selecting its stem parts to extract rutin, and loading rutin with chitosan pellets, which can completely inhibit the growth and reproduction of algae for a long time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0018] Select plump Salicornia seeds and sow them in the vermiculite in the culture bottle. After they germinate, the seawater filtered through a 0.2μm cellulose acetate membrane is injected into the culture bottle according to the volume ratio of vermiculite and seawater at a ratio of 1:3. , and adjust the pH of the cultured seawater to 7.8, the salinity to 30psu, and set the light intensity to 120μmol m -2 the s -1 , the temperature was 20°C, and it was cultivated for 25 days; the planted salicornia and its roots were taken out, rinsed with deionized water, the leaves and excess roots were removed, and it was freeze-dried for 20 hours and then ground into powder, and passed through 200 mesh Sieve and sieve to obtain the salt hornwort stem powder. At the same time, according to the solid-to-liquid mass ratio of salt hornwort stem powder and n-butanol 1:4, stir and mix it at 20°C and 800r / min for 10min, and wait for the stirring to be completed , at 25kHZ, ultrasonically extr...

example 2

[0020] Select plump Salicornia seeds, sow them in the vermiculite in the culture bottle, and after they germinate, pour the seawater filtered through a 0.25μm cellulose acetate membrane into the culture bottle according to the volume ratio of vermiculite and seawater at a ratio of 1:3 , and adjust the pH of the cultured seawater to 8.0, the salinity to 30psu, and set the light intensity to 130μmol m -2 the s -1, the temperature was 25°C, and it was cultivated for 28 days; the planted salicornia and its roots were taken out, rinsed with deionized water, the leaves and excess roots were removed, and it was freeze-dried for 25 hours and then ground into powder. Sieve and sieve to obtain the salt hornwort stem powder, and at the same time according to the solid-to-liquid mass ratio of salt hornwort stem powder and n-butanol 1:4, place it at 25°C and 1000r / min and stir for 12min, after the stirring is completed , at 30kHZ, ultrasonically extracted for 1.5h, and placed in a dark en...

example 3

[0022] Select plump Salicornia seeds, sow them in the vermiculite in the culture bottle, and after they germinate, pour the seawater filtered through a 0.3μm cellulose acetate membrane into the culture bottle according to the volume ratio of vermiculite and seawater at a ratio of 1:3 , and adjust the pH of the cultured seawater to 8.2, the salinity to 30psu, and set the light intensity to 140μmol m -2 the s -1 , the temperature was 30°C, and it was cultivated for 30 days; the planted salicornia and its roots were taken out, rinsed with deionized water, the leaves and excess roots were removed, and it was freeze-dried for 30 hours and then ground into powder. Sieve and sieve to obtain the salt hornwort stem powder. At the same time, according to the solid-to-liquid mass ratio of salt hornwort stem powder and n-butanol 1:4, place it at 30°C and 1200r / min and stir for 15 minutes. After the stirring is completed , at 35kHZ, ultrasonically extracted for 2h, and placed in a dark en...

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Abstract

The invention relates to a preparation method of a salicornia rutin algicide, belonging to the field of alga treatment. Aiming at the problems that the existing physical method for treating water eutrophication algae is not thorough and is easy to cause secondary or multiple outbreaks and the existing biological method is relatively long in treatment time and not obvious in effect, the preparation method of the salicornia rutin algicide comprises the following steps: screening and planting for culturing salicornia, freeze-grinding the stem of the salicornia into powder, carrying out ultrasonic assistance through n-butyl alcohol to extract rutin, dispersing chitosan powder to form chitosan suspension, carrying out deionized water suction filtration and extraction to prepare a chitosan microsphere carrier, and then magnetically stirring the rutin and the chitosan microsphere carrier so as to load the rutin on the chitosan microsphere carrier to prepare the algicide. The inhibition rate is as high as 95-99%, and the algicide prepared by extraction of the salicornia is biodegradable, environment-friendly and free of secondary pollution.

Description

technical field [0001] The invention relates to a preparation method of a salicornia rutin algae inhibitor, which belongs to the field of algae treatment. Background technique [0002] The eutrophication of water bodies has become the focus of human attention in recent years, and it has seriously affected the normal life of human beings. The lakes in the country have experienced different degrees of eutrophication. The control of eutrophication is mainly to suppress or even kill harmful algae, among which blue-green algae is the main target. Cyanobacteria are firstly not picky about temperature, and secondly, they are a kind of "pollution-loving" plant, which leads to their strong competitiveness, which can crowd out other organisms and occupy one side of water and soil. In nature, cyanobacteria have strong competitiveness, which makes them in an advantageous state, and when the water body is seriously polluted, the algae in the water bloom are all cyanobacteria. In recent ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/16A01N25/10A01P13/00C07H17/07C07H1/08
Inventor 卢峰卢斌徐俊伟魏金豹姚梦威沈峰
Owner 上海双骏环保科技有限公司
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