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Colon cancer marker and application thereof

A marker, colorectal cancer technology, applied in the field of biomarkers, can solve the problem of lack of non-invasive early diagnosis methods for colorectal cancer, and achieve the effect of improving and restoring the intestinal microecological structure and preventing defects.

Active Publication Date: 2015-12-09
SHANGHAI REALBIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Colon cancer has no obvious symptoms in the early stage, and there is currently a lack of effective non-invasive early diagnosis methods for colorectal cancer

Method used

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  • Colon cancer marker and application thereof
  • Colon cancer marker and application thereof
  • Colon cancer marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] In this example, the inventors carried out correlation analysis of intestinal flora microorganisms to study the characteristics of fecal microbial communities and functional components from fecal samples of 53 patients with colorectal cancer, 42 patients with colonic adenoma, and 61 healthy individuals. In general, a total of about 1084.87Gb of sequencing data of each sample in the above-mentioned control group was downloaded, and a colorectal cancer reference gene set was constructed. Quantitative metagenomic analysis revealed that 14,993 genes were significantly different (fdr<0.05) in a large number of patients and healthy controls. Most of the genes can be classified into 6 gene clusters (MetagenomicSpecies, MGS) representing bacterial species, of which 2 MGS were enriched in the colorectal cancer patient group, and 4 MGS were enriched in the healthy group.

[0059] 1. Download the sequencing data of the control group

[0060] The DNA sequencing data of stool sampl...

Embodiment 2

[0091] In order to verify that the species markers determined in Example 1 can be used as colorectal cancer markers, the MGS abundances of the remaining 47 healthy people and 31 colorectal cancer patients were used for verification. Wherein, the determination of gene abundance and species (MGS) abundance refers to the steps in the above examples.

[0092] The verification result is as image 3 shown. Taking the significance level α=0.05, the 4 markers determined in Example 1 that are significantly enriched in the healthy population have a significant difference in the abundance of the healthy group and the disease group in the verification group (P<0.05), as shown in Table 3. Eubacterium halllii is Enterobacter hallii, and it has been reported that enterobacterium hallii in the termite gut has a decomposing effect on lignin. Ruminococcus belongs to the genus Ruminococcus. Clostridium is the genus Clostridium and Eubacterium is the genus Eubacterium.

[0093] Taking the si...

Embodiment 3

[0097] In order to further verify whether the species markers determined in Example 1 can be used as colorectal cancer markers, stool samples from 54 colorectal cancer patients and 66 healthy individuals were obtained. The extraction of DNA in stool samples and the acquisition of sequencing data were carried out according to the instructions of the commercially available DNA extraction kits purchased and the instructions for library construction and machine use of the selected sequencing platform. The determination of gene abundance and species abundance is carried out with reference to the above examples.

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Abstract

The invention discloses a colon cancer marker. The colon cancer marker comprises the microorganisms of rare pediococcocci Subdoligranulum_4_3_54A2FAA and polymorph bacilli Bacteroidses. The invention further discloses a method and device for determining individual states through the colon cancer marker. Compared with healthy individuals and groups, the colon cancer marker is remarkably enriched in patient groups with colon cancer, thereby being capable of serving as a distinguishing mark for healthy groups and patient groups with colon cancer.

Description

technical field [0001] The present invention relates to the field of biomarkers. Specifically, the present invention relates to colorectal cancer biomarkers and applications thereof. More specifically, the present invention relates to a colorectal cancer marker, the use of colorectal cancer markers, and a method for using colorectal cancer markers. A method for determining the state of an individual using a colorectal cancer marker, a device for determining the state of an individual using a colorectal cancer marker, a method for classifying a plurality of individuals using a colorectal cancer marker, a drug for treating colorectal cancer, and a method for preparing a treatment Approaches to colorectal cancer drugs. Background technique [0002] Colorectal cancer (Colorectal Cancer, CRC) is a cancer originating from the colon or rectum (a part of the large intestine). Due to abnormal growth of cells, it may invade or metastasize to other parts of the body. Patients with col...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12Q1/68C12Q1/02G06F19/22C12R1/145C12R1/01
Inventor 仲文迪刘婉辉郑智俊
Owner SHANGHAI REALBIO TECH CO LTD
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