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A lactobacillus fermentum strain Lee capable of preventing gastric ulcer and its health care application

A technology for fermenting lactobacillus and gastric ulcer, applied in the field of microorganisms

Inactive Publication Date: 2019-04-16
SOUTHWEST UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these patents or patent applications do not fully address the microorganisms having gastric ulcer preventive properties or their functions

Method used

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  • A lactobacillus fermentum strain Lee capable of preventing gastric ulcer and its health care application
  • A lactobacillus fermentum strain Lee capable of preventing gastric ulcer and its health care application
  • A lactobacillus fermentum strain Lee capable of preventing gastric ulcer and its health care application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1: Isolation, purification and preliminary identification of Lactobacillus fermentum strain Lee.

[0052] This embodiment is carried out according to the following steps:

[0053] (1) Separation and purification of Lactobacillus fermentum strain Lee.

[0054] Aseptically draw 500 μL of traditional fermented yak yogurt sample and add it to 5 mL sterilized saline to make a uniform dilution of 1:10, and continue to make a certain proportion of dilution. Select the appropriate gradient diluent, draw 100 μL each into the MRS solid plate medium with a sterile pipette tip, and incubate at 30°C for 48-72 hours, observe and record the colony morphology as follows: figure 1 shown.

[0055] Use an inoculation loop (or a sterilized toothpick) to pick different colonies from the surface and interior of the plate and inoculate them in the MRS liquid medium, and culture them on a shaker at 30°C and 300r / min for 24-48h; repeat the above steps for continuous activation for 2 g...

Embodiment 2

[0061] Example 2: In vitro screening of Lactobacillus fermentum strain Lee.

[0062] (1) Screening of probiotics resistant to pH 3.0 artificial gastric juice.

[0063] Preparation of artificial gastric juice: 0.2% NaCl, 0.35% pepsin, adjust the pH value to 3.0 with 1M HCl, filter and sterilize with a vacuum pump in an aseptic operating table for later use.

[0064] Determination of the tolerance of probiotics to artificial gastric juice: take 5mL of the activated strain culture solution, pour it into a sterilized 10mL centrifuge tube in a sterile operating table, collect the bacteria by centrifugation at 3000r / min for 10min, add 5mL of sterilized Mix physiological saline to make bacterial suspension, take 1mL bacterial suspension and 9mL artificial gastric juice with pH3. Samples were taken, poured into MRS agar medium and cultured at 37°C for 48h. The number of viable bacteria was determined by plate counting method, and the survival rate (%) was calculated: survival rate (...

Embodiment 3

[0078] Example 3: Experiment of gastric ulcer induced by reserpine.

[0079] Feed 50 healthy SPF grade Kunming mice, weighing 23±2g, male. After being fed with basic feed for 7 days, the mice were randomly divided into 5 groups according to body weight, namely: normal group, gastric ulcer control group, omeprazole group, Lactobacillus bulgaricus group and Lactobacillus fermentum strain Lee group, with 10 mice in each group. Raise them in stainless steel cages, keep room temperature at 24±2°C, relative humidity at 50±10%, and light-dark rotation for 12 hours (lighting at 8:00-20:00). During the whole experimental period, the normal group and the gastric ulcer control group were fed with 0.2mL normal saline every day, and the experimental mice in the Lactobacillus bulgaricus group and the Lactobacillus fermentum strain Lee group were fed with 1.0×10 9 CFU / kg gavage 0.2mL of the corresponding bacterial solution. The omeprazole group was orally administered 0.2mL of omeprazole d...

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Abstract

The invention discloses lactobacillus fermentum Lee capable of preventing gastric ulcers and health-care application of the lactobacillus fermentum Lee. A bacterial strain of which the preservation number is CCTCCM2013512 has strong acid resistance, and the survival rate in artificial gastric juice of which the pH value is 3.0 for three hours reaches 40.48 plus or minus 1.84%, and the survival rate in cholate of which the concentration is 0.3% reaches 60%; the hydrophobicity of lactobacillus fermentum Lee cells also reaches 15.17 plus or minus 6.20%, and the lactobacillus fermentum Lee cells can normally grow in human intestines. By adopting the lactobacillus fermentum Lee, the areas and degrees of the gastric ulcers can be reduced; the secretion of gastric acid is reduced; the levels of somatostatin (SS) and vasoactive intestinal peptide (VIP) factors can be increased by different degrees; the levels of motilin (MOL) and P substance (SP) factors are reduced, and the levels of interleukin-6 (IL-6), interleukin-12 (IL-12), tumor necrosis factors-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) are lowered. The effect similar to omeprazole for treating the gastric ulcers is achieved, and a certain gastric ulcer preventing effect is shown.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Lactobacillus fermentum strain Lee capable of preventing gastric ulcer and its application. Background technique [0002] Lactobacillus is closely related to human life and is one of the beneficial microorganisms widely used in food fermentation, industrial lactic acid fermentation and medical care. Many of the now recognized Lactobacillus and Bifidobacterium genera have specific favorable properties and genetic traits, showing extremely strong probiotic properties. Lactobacillus can not only improve the nutritional value of food, improve and give food flavor, reduce the addition of flavors and fragrances in food, prolong the storage time, but also improve the functional properties of food. It can improve the digestibility and biological value of food by decomposing protein, sugar and synthetic vitamins in food, promote digestion and absorption, prevent constipation, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23C9/127A23L33/135C12R1/225
Inventor 李键索化夷骞宇赵欣兰道亮陈炼红李明月
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES
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