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Method for producing functional microbes with glutathione waste liquid

A technology of functional microorganisms and glutathione, applied in the field of microorganisms, can solve the problems of high capital construction investment and operation and maintenance costs, low utilization rate of waste recycling, and unreported research results, so as to improve the comprehensive utilization rate and added value. , Solve the effect of unstable product performance and quality, and solve the problem of environmental pollution

Active Publication Date: 2015-12-09
山东君德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Direct discharge of various waste liquids will not only seriously pollute the environment, but also cause waste of resources
Using traditional physicochemical treatment technology and biological treatment technology such as coagulation method, evaporation concentration method, adsorption method, activated sludge method, biological activated carbon method, biofilm method, etc. to treat wastewater, although it can meet the standard, the capital investment and operation and maintenance costs are high. , the process is complicated, the recycling rate of waste is low, and it is easy to produce secondary pollution
[0006] The recycling of glutathione waste liquid is the best strategy for the sustainable development of glutathione enterprises, but the relevant research results have not been reported yet

Method used

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  • Method for producing functional microbes with glutathione waste liquid
  • Method for producing functional microbes with glutathione waste liquid

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0036] Bacillus licheniformis (provided in the form of ampoule freeze-dried powder) was purchased from the China Industrial Microorganism Culture Collection Management Center, number CICC23584;

[0037] Lactobacillus acidophilus (provided form: freeze-dried powder in ampoules) was purchased from China Industrial Microorganism Culture Collection Management Center, No. CICC6074);

[0038] Candida utilis (provided in the form of ampoule freeze-dried powder) was purchased from the China Industrial Microorganism Culture Collection Management Center, No. CICC31188);

[0039] Bacillus subtilis (provided in the form of ampoule freeze-dried powder) was purchased from China Industrial Microorganism Culture Collection Management Center, number CICC20872).

[0040] 1) Activation of strains

[0041] (1-1) Activation of Bacillus licheniformis

[0042] Slant medium preparation: 0.5% peptone, 0.3% beef extract, 1.5% NaCl, 1.5% agar, pH 7.0, 121°C, 0.103MPa, sterilized for 30min, and prepare...

preparation Embodiment 2

[0088] Horizontal twin-shaft paddle mixer model SSHJ-4, total volume 4m 3 , each batch of mixing capacity is 2 tons, wherein the bran (water content 8.5%) of filling 1.7 tons, fermented liquid 0.3 tons, mixing time 60s, the material moisture that comes out from the mixer is 21.58%, Bacillus licheniformis bacteria content 2.60 ×10 8 CFU / mL; Bacillus subtilis content 2.54×10 8 CFU / mL; Candida utilis bacteria content 2.72×10 8 CFU / mL; Lactobacillus acidophilus content 4.87×10 8 CFU / mL; total number of viable bacteria 12.73×10 8 CFU / mL

[0089] Put the materials coming out of the mixer into trays, the depth of each tray is 4cm, send the trays to the three-dimensional rack of the drying room, the drying room is 12m×12m×3m, turn over the materials every 1h, and dry The drying room temperature is controlled at 37°C, and the drying time is 20 hours. 1.7 tons of dried solid functional microbial products are obtained. 8 CFU / mL; Bacillus subtilis content 2.26×10 8 CFU / mL; Candida ...

experiment example 1

[0091] A comparative test was carried out with the liquid type bottled functional microorganism product obtained in Preparation Example 1 and the commercially available products produced by other companies.

[0092] Test site: located in the lettuce greenhouse of Jinan Agricultural High-tech Industry Demonstration Park

[0093] Trial period: September 30, 2014 to December 18, and March 1, 2015 to May 11. Sowing seedlings were carried out on September 30, 2014 and March 1, 2015; planting was carried out on October 28, 2014 and March 25, 2015, respectively, and the planting density was 20-25 cm in rows and plants; in December 2014 Harvested on the 10th and May 15th, 2015 respectively.

[0094] Varieties of lettuce to be tested: American fast-growing.

[0095] Test bacterial fertilizer and test design:

[0096] The liquid-type bottled functional microbial product obtained in Example 1 of the present invention is represented by A; Runkangyuan microbial bacterial agent (the stra...

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Abstract

The invention discloses a method for producing functional microbes with glutathione waste liquid and belongs to the field of microbes. The method includes: a, respectively subjecting bacillus licheniformis seed solution, candida utilis seed solution, lactobacillus acidophilus seed solution, lactobacillus acidophilus seed solution and bacillus subtilis seed solution to seeding tank culture by taking the glutathione waste liquid as an effective ingredient of a seed culture medium; b, performing fermentation in a fermentation tank by taking the glutathione waste liquid as an effective ingredient of a fermentation tank culture medium to obtain functional fermentation broth. Compared with the prior art, the method has the advantages that the functional microbes are produced with the glutathione waste liquid, and the method is low in cost, diversified in product, high in economic value, high in waste recycling rate and the like and has extensive application value.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a method for producing functional microorganisms using glutathione waste liquid. Background technique [0002] Glutathione, generally refers to reduced glutathione (Glutathione, GSH), which is a biologically active substance widely present in normal cells of the human body, and is the most abundant sulfhydryl-containing low-lying compound naturally synthesized in human cells. Molecular tripeptide, clinically proven that reduced glutathione is a widely used small molecule peptide active substance, which has important anti-oxidation and integrated detoxification effects, and is used for viral, drug toxicity, alcohol toxicity and other chemical substances. It is also an adjuvant therapy drug for cancer patients during radiotherapy and chemotherapy, and has a positive effect on the combined treatment of acute anemia, adult respiratory distress syndrome, and sepsis. [0003] At present,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12R1/10C12R1/23C12R1/125C12R1/72
Inventor 常钟钱朋智史晓洁蔡洋赵传涛侯大彬
Owner 山东君德生物科技有限公司
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