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Method for preparing tissue-inducing hydrogels for cartilage repair

A cartilage repair and hydrogel technology, which is applied in the fields of medical science and prosthesis, can solve the problems of expensive growth factors, short half-life, and activity damage, and achieve the advantages of growth and repair, simple preparation, and resistance to damage. powerful effect

Inactive Publication Date: 2018-08-17
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, growth factors are expensive, have a short half-life, and are easily denatured. Dropping growth factors directly on the surface of the hydrogel or directly swelling the solution containing the factors with the hydrogel is prone to burst release, and may also cause toxicity and carcinogenicity due to local overdose
The problem of burst release and local excess toxicity can be solved by covalently immobilizing the factor in the group of the hydrogel component, but the activity of the factor will be destroyed by the covalent reaction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A. Double Bonding of Gelatin

[0028] Gelatin was dissolved in phosphate buffered saline to obtain a solution with a gelatin mass concentration of 10%, then, methacrylic anhydride 0.8 times the mass of gelatin was added dropwise to the solution, and then stirred and reacted for 3 hours at 50°C; subsequently, The solution was diluted 5 times, then dialyzed and dried to obtain double-bonded gelatin;

[0029] B. Preparation of hydrogel

[0030] Dissolving the methacrylamidated dopamine monomer in dimethyl sulfoxide to obtain a methacrylamidated dopamine monomer solution, and then co-dissolving the methacrylamidated dopamine monomer solution and the double bonded gelatin obtained in step A in A mixed solution is prepared in the phosphate buffer solution, the mass concentration of the double-bonded gelatin in the mixed solution is 20%, and the mass concentration of the methacrylamide dopamine monomer is 4%. Then, the photoinitiator irgacure2959 is added to the mixed soluti...

Embodiment 2

[0034] A. Double Bonding of Gelatin

[0035] Dissolving the gelatin in a phosphate buffer solution to obtain a solution with a gelatin mass concentration of 10%, then adding glycidyl methacrylate 17.5 times the mass of the gelatin dropwise to the solution, and then stirring and reacting at 50°C for 3 hours; Subsequently, the solution was diluted 5 times, then dialyzed and dried to obtain double-bonded gelatin;

[0036] B. Preparation of hydrogel

[0037] Dissolving the methacrylamidated dopamine monomer in dimethyl sulfoxide to obtain a methacrylamidated dopamine monomer solution, and then co-dissolving the methacrylamidated dopamine monomer solution and the double bonded gelatin obtained in step A in A mixed solution is prepared in the phosphate buffer solution, the mass concentration of the double-bonded gelatin in the mixed solution is 15%, and the mass concentration of the methacrylamide monomer is 3%. , the photoinitiator irgacure127 is added to the mixed solution, the ...

Embodiment 3

[0041] A. Double Bonding of Gelatin

[0042]Dissolving the gelatin in a phosphate buffer solution to obtain a gelatin solution with a mass concentration of 10%, then adding glycidyl methacrylate 0.16 times the mass of the gelatin dropwise to the solution, and then stirring and reacting at 50°C for 3 hours; Subsequently, the solution was diluted 5 times, then dialyzed and dried to obtain double-bonded gelatin;

[0043] B. Preparation of hydrogel

[0044] Dissolving the methacrylamidated dopamine monomer in dimethyl sulfoxide to obtain a methacrylamidated dopamine monomer solution, and then co-dissolving the methacrylamidated dopamine monomer solution and the double bonded gelatin obtained in step A in A mixed solution is prepared in the phosphate buffer solution, the mass concentration of the double-bonded gelatin in the mixed solution is 20%, and the mass concentration of the methacrylamide monomer is 5%. , the photoinitiator irgacure184 is added to the mixed solution, the m...

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Abstract

The invention discloses a method for preparing a tissue-inducing hydrogel for cartilage repair, the steps of which mainly include: A, double bond of polysaccharide or protein, B, preparation of hydrogel, C, growth factor of adsorption. This method uses fully degradable double bonded natural polysaccharide / protein and methacrylamide dopamine as the main components, and forms a double network structure through free radical polymerization of double bonded polysaccharide / protein and methacrylamide dopamine. Hydrogels. Subsequently, the hydrogel adsorbs biologically active growth factors through dehydration and reswelling, and the growth factors are immobilized in the hydrogel by the dopamine component. The hydrogel prepared by the method has good biodegradability and cell / tissue affinity, and the loaded growth factor has high activity and good slow-release performance, and can better induce cartilage regeneration and promote cartilage repair.

Description

technical field [0001] The invention relates to a method for preparing a tissue-inducing hydrogel for cartilage repair Background technique [0002] The structure of hydrogel is similar to the extracellular matrix of natural cartilage. It has been widely used in the research of cartilage defect repair and is expected to become a cartilage replacement material. However, the poor mechanical properties of hydrogels make it difficult to adapt to various mechanical environments in joints, which limits their application as cartilage replacement materials. The latest research has found that the double-network structure hydrogel has good mechanical properties, and can even make the mechanical properties of the hydrogel reach or exceed those of cartilage. The existing preparation method of the double network structure hydrogel is mainly to first polymerize a monomer network, then put it into the solution containing the second layer network monomer to swell and then polymerize the se...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/24A61L27/20A61L27/52A61L27/54C08F290/10C08F290/00
Inventor 鲁雄胥杰龙甘东林王振铭张晏宁
Owner SOUTHWEST JIAOTONG UNIV
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