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Graphene-modified lactic acid biosensor and preparation method thereof

A biosensor, graphene technology, applied in the field of biosensors, can solve problems such as affecting the accuracy and sensitivity of biosensors, hindering electron transfer, etc., to avoid electrochemical interaction, realize miniaturization, and realize the effect of integration

Active Publication Date: 2018-04-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for most redox proteins or enzymes, the active center is usually buried in a non-conductive protein structure, which hinders the transfer of electrons, thus affecting the accuracy and sensitivity of biosensors

Method used

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  • Graphene-modified lactic acid biosensor and preparation method thereof
  • Graphene-modified lactic acid biosensor and preparation method thereof
  • Graphene-modified lactic acid biosensor and preparation method thereof

Examples

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Embodiment 1

[0051] The preparation steps of the lactic acid biosensor modified by graphene of the present invention are as follows:

[0052] (1) Using the screen printing method, use silver paste to print on the polypropylene substrate such as figure 1 For the silver wire of the reference electrode shown, the mesh cloth used is 420 mesh, the mesh distance is 1.5mm, the scraper hardness is 80°, the scraper angle is 64.3°, the scraper speed is 8mm / s, and the scraper pressure is 4kg / cm 2After printing, dry the substrate in the oven for 2 minutes in the dark, and the oven temperature is 50°C to form a dry silver wire;

[0053] (2) Using the method of screen printing, use carbon paste to print on the polypropylene substrate such as figure 1 The carbon paste wire and the counter electrode of the working electrode shown are 420-mesh mesh cloth, 1.5mm mesh distance, 80° scraper hardness, 64.3° scraper angle, 8mm / s scraper speed, and 4kg / scraper pressure. cm 2 After printing, dry the substrate...

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Abstract

The invention discloses a graphene-modified lactic acid biosensor, comprising: a substrate, a reference electrode, a counter electrode and a working electrode arranged in parallel on the substrate, and coated on the reference electrode, the counter electrode and the working electrode An insulating layer on part of the surface, the insulating layer and the exposed part of the reference electrode, the counter electrode and the working electrode surround a reaction chamber, and the working electrode includes a carbon paste wire, a graphene layer and an enzyme solution layer, The graphene layer is covered on the reaction area of ​​the carbon paste wire; the enzyme liquid layer is located in the reaction chamber and combined with the graphene layer, and the enzyme liquid layer contains lactate oxidase and enzyme activity additives. The lactic acid biosensor is a miniaturized lactic acid sensor that does not rely on electron transfer agents, has low cost, high sensitivity, and good anti-interference performance, and can quickly and accurately measure the lactic acid content in human serum.

Description

technical field [0001] The invention belongs to the field of biosensors, in particular to a graphene-modified lactic acid biosensor and a preparation method thereof. Background technique [0002] Lactic acid is an intermediate product of carbohydrate metabolism. The detection of lactic acid content in the blood can reveal the severity of certain diseases. When human tissue is hypoxic or suffers from certain liver diseases, the concentration of lactic acid in the blood will increase. The detection of lactic acid content in blood can also help guide athletes to carry out reasonable training. The normal concentration of lactic acid in human blood is 1.2-2.7mmol / L, but the concentration can reach 25mmol / L after intense exercise. The above characteristics make lactic acid can be used as a relevant indicator in clinical and sports medicine, so it is very important to quickly, easily and accurately determine the content of lactic acid in blood. [0003] Lactate biosensors are gen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/327
Inventor 胡瑞芬屠丹丹李光
Owner ZHEJIANG UNIV
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