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Preparation method and application of graphene based hemoglobin sensor electrode

A sensor electrode and hemoglobin technology, applied in the field of biosensors, can solve problems such as low occult blood, achieve easy adsorption, improve the effect of enrichment and direct electrochemical reaction, and achieve the effect of direct detection

Active Publication Date: 2018-07-03
INST OF METAL RESEARCH - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the concentration of fecal occult blood is usually low, and it is difficult to observe with the naked eye, so a process of enrichment and direct electrochemical determination is required

Method used

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  • Preparation method and application of graphene based hemoglobin sensor electrode
  • Preparation method and application of graphene based hemoglobin sensor electrode

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preparation example Construction

[0034] In the specific implementation process, the preparation method of a graphene-based third-generation hemoglobin sensor proposed by the present invention firstly drips a graphene oxide solution onto the cleaned sensor electrode, and electrochemically reduces the graphite oxide. Graphene is converted into graphene for direct electron transfer of hemoglobin and active adsorption and enrichment on the electrode surface. Then, the chitosan solution was drop-coated on the surface of the electrode, and after drying at room temperature, the hemoglobin sensor electrode modified with graphene was obtained. The role of chitosan is to prevent graphene from falling off into the electrolyte and improve the stability of the sensor. The specific steps of this method are as follows:

[0035] (1) Preparation of the third-generation hemoglobin sensor of graphene:

[0036] Pretreatment of glassy carbon electrode: First, the glassy carbon electrode A1 with particle sizes of 1.0 μm, 0.3 μ...

Embodiment 1

[0043] First, a PBS solution with a concentration of 1 mg / ml graphene oxide and a chitosan acetic acid solution with a concentration of 0.2 wt% were prepared respectively. Then, the electrode 1 whose surface has been polished is modified. First, 1 μl of the above graphene oxide solution is dropped on the electrode, and after the electrode is dried at room temperature, 5 μl of 0.2 wt% chitosan solution is added dropwise. After the electrode is dried, the electrode is inserted into a 0.1 mol / L phosphate buffer solution and soaked for 2 hours to remove the graphene oxide that is not firmly bonded to the electrode, and finally a graphene oxide modified electrode is obtained. The above-mentioned modified electrode is used as the working electrode, and a three-electrode system is formed with a saturated calomel electrode (reference electrode) and a platinum sheet electrode (counter electrode), and a phosphate buffer solution of 0.1 mol / L is used as the electrolyte. Electrochemical r...

Embodiment 2

[0045] The preparation method of the graphene-modified electrode is the same as that of Example 1. In order to investigate the electrode reaction process of hemoglobin on the graphene-modified electrode, the modified electrode is used as the working electrode and the silver / silver chloride reference electrode. In the electrode system, 0.06M sulfuric acid containing 2 μg / mL hemoglobin was used as the electrolyte, and the cyclic voltammetry curves were tested at different scanning speeds. The voltage scanning rate is proportional, indicating that the electrode process is controlled by the electron transfer process on the electrode surface, that is, the adsorption process.

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Abstract

The invention belongs to the field of biosensors and specifically relates to a preparation method and application of a graphene based hemoglobin sensor electrode. Graphene and chitosan are compoundedto serve as a modification layer of an electrode; excellent biocompatibility and conductivity of the graphene facilitate direct electron transfer between hemoglobin and an electrode; and due to the excellent biocompatibility of the graphene, the agglomerated hemoglobin molecules are spread on the surface of the graphene, and the heme at the electroactive part is exposed, to realize direct electrontransfer between the hemoglobin and the electrode as well as direct detection of hemoglobin. According to the hemoglobin biosensor prepared in the invention, the graphene material is favorable for adsorbing and spreading hemoglobin, and also can realize direct electron transfer with the electrode; the biosensor has the advantages of high sensitivity, high stability, wide linear range and relatively strong anti-jamming capability, and can be widely applied to fecal occult blood screening of suspected gastrointestinal cancer patients.

Description

technical field [0001] The invention belongs to the field of biosensors, and in particular relates to a preparation method and application of a graphene-based hemoglobin biosensor electrode. Background technique [0002] Colorectal cancer is a common malignant tumor in the gastrointestinal tract, accounting for the first place in all gastrointestinal cancers, and its incidence is increasing year by year. According to the National Cancer Registry, the incidence of colorectal cancer from 1991 to 2005 was one of the three fastest-rising tumors. In Western societies, the incidence is higher. According to recent statistics, there is one case of colorectal cancer in every 20 adults. Foreign data show that colorectal cancer screening can detect precancerous diseases, and after appropriate intervention, the morbidity and mortality of tumors can be effectively reduced. Although the diagnostic technology continues to develop, because the early symptoms of colorectal cancer are easy ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/327
Inventor 英哲张睿杨卫东郝亚斌孙宝明宋纯李记彬曾尤成会明
Owner INST OF METAL RESEARCH - CHINESE ACAD OF SCI
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