Xenopus smooth skin antibacterial peptide and preparation method and use thereof

Abstract

An antimicrobial peptide of Xenopus laevis and its preparation method and application. The antibacterial peptide is isolated from the skin secretion of Xenopus laevis. After purification and sequencing, its sequence is Asp‑Glu‑Asp‑Asp‑Asp (DEDDD), and its mass spectrometry The molecular weight is 607.7 Da. The invention uses a physical method to stimulate the skin of Xenopus laevis, which greatly protects the structural integrity and biological activity of the antimicrobial peptide. The antimicrobial peptide has significant antibacterial activity, low hemolytic activity and good anti-breast cancer activity. Its molecular weight is low, which reduces the difficulty of its chemical synthesis and greatly reduces the cost. It has significant antibacterial effect on Gram-positive bacteria and Gram-negative bacteria, and has extremely low hemolytic activity within the MIC value range, and has high anti-breast cancer activity, which can be used for the prevention of wound infection , anti-tumor, antibacterial agents and other medical directions, with good application prospects.

Description

technical field [0001] The invention relates to the technical field of medicinal chemistry, in particular to a smooth Xenopus skin antibacterial peptide and its preparation method and application. Background technique [0002] Antibacterial peptides, also known as antimicrobial peptides, are substances with antimicrobial activity released by the immune system, which widely exist in organisms in nature as part of the innate immune components. Antimicrobial peptides are small molecule polypeptide substances with a wide range of biological activities, such as sterilization, anti-inflammatory, anti-virus, and tumor-inhibiting effects. They are an important part of biological non-specific defense systems. Antimicrobial peptides have broad-spectrum antibacterial activity, and can combine with lipopolysaccharide on the outer membrane of Gram-negative bacteria and peptidoglycan on the surface of Gram-positive bacteria, thereby destroying the cell membrane to form transmembrane chann...

AI Technical Summary

Problems solved by technology

The above two antimicrobial peptides are difficult and costly to synthesize due to their long sequences and secondary structures.

In the Chinese Patent Publication No. 103524602A, a six-peptide antibacterial peptide with the sequence RRRWWC is disclosed. However, this invention only clarifies its viral activity, and does not clarify its anti-tumor and other effects, and the patent does not study the antibacterial peptide. The hemolytic activity of the peptide greatly limits its application

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