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Application of IL-17 in improving mesenchymal stem cell immunity inhibition function

An immunosuppressive, stem cell technology, applied in the field of biomedicine

Inactive Publication Date: 2015-11-25
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although IL-17 plays a protective role in the body against bacterial and fungal infections, overactivation of the IL-17 signaling pathway can lead to autoimmune diseases

Method used

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  • Application of IL-17 in improving mesenchymal stem cell immunity inhibition function
  • Application of IL-17 in improving mesenchymal stem cell immunity inhibition function
  • Application of IL-17 in improving mesenchymal stem cell immunity inhibition function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1I

[0284] Example 1 IL-17 can enhance the immunosuppressive function of MSC

[0285] Studies have shown that the immunosuppressive ability of mesenchymal stem cells is not inherent, but acquired under the induction of inflammatory cytokines, including IFNγ, TNFα, IL-1α and IL-1β. Under the stimulation of inflammatory cytokines IFNγ and TNFα, mesenchymal stem cells secrete a large amount of iNOS and chemokines. Chemokines recruit T cells to the surroundings of mesenchymal stem cells, and the metabolite NO of iNOS exerts the effect of inhibiting T cell proliferation. effect. As we all know, IL-17 is a pleiotropic pro-inflammatory cytokine, which plays an important role in the pathogenesis of many infectious diseases, inflammatory diseases and autoimmune diseases. The present inventors added inflammatory cytokines IFNγ and TNFα to mesenchymal stem cells cultured in vitro, or added IL-17 on this basis, and observed the effects of these cytokines on the immunosuppressive ability of m...

Embodiment 3I

[0297] Example 3 IL-17 enhances the immunosuppressive function of MSC independent of the effect on T cell apoptosis

[0298]The above studies have shown that IL-17 can cooperate with inflammatory cytokines IFNγ and TNFα to enhance the immunosuppressive function of MSC, and this function is mainly reflected in the inhibition of T cell proliferation and depends on the activity of iNOS (Figure 1, Figure 2) . In addition, MSCs can induce lymphocyte apoptosis in the presence of inflammatory cytokines IFNγ and TNFα, and this process also depends on NO. In order to determine whether this enhanced MSC immunosuppressive effect of IL-17 is dependent on the effect on T cell apoptosis, the inventors stimulated MSCs with IFNγ+TNFα or IFNγ+TNFα+IL-17 for 12 hours in advance, and then stimulated them Co-culture with A1.1 cells at a ratio of 1:10, collect A1.1 cells after 12 hours, and detect cell apoptosis. The inventors found that the proportion of apoptotic cells was lower than 0.5% when...

Embodiment 4I

[0301] Example 4 IL-17 cooperates with inflammatory cytokines to up-regulate the expression of iNOS in MSCs

[0302] Previous studies have shown that iNOS and chemokines are the core molecules that mediate the immunosuppressive function of MSCs, and the induction of inflammatory cytokines IFNγ and TNFα is required to initiate the expression of iNOS and some chemokines in MSCs. The above results of the present inventors show that the ability of IL-17 to enhance the immunosuppression of MSCs depends on the activity of iNOS. The present inventors treated MSCs with IFNγ+TNFα or IFNγ+TNFα+IL-17, and then measured the expressions of iNOS and chemokines by fluorescent quantitative PCR and Western Blot. The results showed that IL-17 could cooperate with IFNγ and TNFα to significantly promote the expression of iNOS ( Figure 4A , B, C, D). At the same time, the inventors also detected the mRNA expression of some chemokines related to MSC immunosuppression, including CCL2, CCL5, CXCL9...

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Abstract

Provided are uses of IL-17 in enhancing an immune-suppression function of mesenchymal stem cells. In particular, the present invention provides uses of an interleukin-17, a derivative of interleukin-17 or an agonist thereof for preparing a preparation or kit for enhancing an immune-suppression function of the mesenchymal stem cells; up-regulating the expression of imunosuppressive factors in the mesenchymal stem cells; enhancing the stability of mRNAs of the immunosuppressive factors; reducing the expression level of RNA-binding protein AUF1; inhibiting the proliferation of T cells; and treating hepatitis or liver damage.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular, the invention relates to the application of IL-17 in improving the immunosuppressive function of mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells (MSCs) are an important member of the stem cell family, derived from the mesoderm and ectoderm in the early stages of development, and belong to pluripotent stem cells. MSCs were originally found in the bone marrow because of their multi-directional differentiation potential, hematopoietic support and The promotion of stem cell engraftment, immune regulation and self-replication has attracted increasing attention. For example, mesenchymal stem cells can be differentiated into fat, bone, cartilage, muscle, tendon, ligament, nerve, liver, cardiac muscle, endothelial and other tissue cells under specific induction conditions in vivo or in vitro, after continuous subculture and cryopreservation It still has multi-direct...

Claims

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Application Information

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IPC IPC(8): A61K38/20A61P37/06A61P1/16A61P31/12A23L1/29C12N5/0775C12N5/10C07K19/00G01N33/68A23L33/00
CPCA61K38/20A61K38/21A61K45/00C07K19/00C12N5/10G01N33/68
Inventor 时玉舫韩晓燕王莹
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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