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A kind of extraction process of honeysuckle polysaccharide

A technology of honeysuckle polysaccharide and extraction process, which is applied in the field of sugar processing technology, can solve the problems of low extraction rate and low quality of honeysuckle polysaccharide, achieve the effects of increasing extraction rate, increasing yield and antioxidant activity, and reducing the decline in antioxidant capacity

Active Publication Date: 2019-03-26
GUANGXI DONGXING DINGKANG PLASTIC IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, the purpose of the present invention is to provide a kind of extraction process of honeysuckle polysaccharide, to solve the existing shortages of low extraction rate and low quality of honeysuckle polysaccharide when extracting honeysuckle polysaccharide in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of extraction technology of honeysuckle polysaccharide, comprises the following steps:

[0023] (1) Drying and crushing: first put the honeysuckle in a microwave vacuum dryer, dry the honeysuckle to a moisture content of 3% under the conditions of a pressure of 10KPa and a temperature of 20°C, and then crush the honeysuckle to a fineness of 60 meshes. Powder, get honeysuckle powder;

[0024] (2) Enzymolysis: add water to honeysuckle powder, and add hydrochloric acid to adjust the pH value of the solution to 6.2, then add an enzymolysis agent, hydrolyze for 25 minutes at a temperature of 40°C, and obtain an enzymolysis solution, the enzymolysis agent is Cellulase, papain, pectinase and glucoamylase, and the weight ratio of cellulase, papain, pectinase and glucoamylase is 4:3:3:5, and the amount of enzymolysis agent is 0.03 of honeysuckle pulp %;

[0025] (3) Ultrasonic treatment: send the enzymatic hydrolysis solution into the ultrasonic equipment, treat it for ...

Embodiment 2

[0031] A kind of extraction technology of honeysuckle polysaccharide, comprises the following steps:

[0032] (1) Drying and crushing: first put the honeysuckle in a microwave vacuum dryer, dry the honeysuckle to a moisture content of 6% under the conditions of a pressure of 18KPa and a temperature of 30°C, and then crush the honeysuckle to a fineness of 200 meshes. Powder, get honeysuckle powder;

[0033] (2) Enzymolysis: add water to honeysuckle powder, and add hydrochloric acid to adjust the pH value of the solution to 6.8, then add an enzymolysis agent, hydrolyze for 40min at a temperature of 60°C, and obtain an enzymolysis solution, the enzymolysis agent is Cellulase, papain, pectinase and glucoamylase, and the weight ratio of cellulase, papain, pectinase and glucoamylase is 4:3:3:5, and the amount of enzymolysis agent is 0.04 of honeysuckle pulp %;

[0034] (3) Ultrasonic treatment: send the enzymatic hydrolysis solution into the ultrasonic equipment, treat it for 30 m...

Embodiment 3

[0040] A kind of extraction technology of honeysuckle polysaccharide, comprises the following steps:

[0041] (1) Drying and crushing: first put the honeysuckle in a microwave vacuum dryer, dry the honeysuckle to a moisture content of 4% under the pressure of 14KPa and a temperature of 25°C, and then crush the honeysuckle to a fineness of 80 meshes. Powder, get honeysuckle powder;

[0042] (2) Enzymolysis: add water to honeysuckle powder, and adjust the pH value of the solution to 6.5, then add an enzymolysis agent, hydrolyze at a temperature of 50°C for 30 minutes, and obtain an enzymolysis solution, and the enzymolysis agent is cellulose enzyme, papain, pectinase and glucoamylase, and the weight ratio of cellulase, papain, pectinase and glucoamylase is 4:3:3:5, and the amount of enzymolysis agent is 0.04% of honeysuckle pulp;

[0043] (3) Ultrasonic treatment: send the enzymatic hydrolysis solution into the ultrasonic equipment, treat it for 25 minutes under the condition o...

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Abstract

The invention discloses an extraction process of lonicera japonica polysaccharide. The process includes: firstly drying lonicera japonica with a microwave vacuum drier, then performing crushing, adding water and acid into the lonicera japonica powder to make a solution, then adding an enzymolysis agent to perform hydrolysis, sending the enzymolysis solution into ultrasonic equipment to conduct ultrasonic treatment and filtering, then sending the filtrate into ultrafiltration equipment to perform ultrafiltration treatment, then sending the ultrafiltrate into a centrifugal machine to carry out centrifugal separation, concentrating the centrifugate, adding ethanol into the concentrate and performing standing treatment, then conducting centrifugal separation and drying the lonicera japonica polysaccharide solution, thus obtaining lonicera japonica polysaccharide. By adopting the microwave low temperature vacuum drying and ultrasonic wave synergistic treatment technology, the process provided by the invention ensures effective wall breaking, and significantly improves the yield and antioxidant activity of lonicera japonica polysaccharide, the extraction rate of lonicera japonica polysaccharide is over 18.6%, the lonicera japonica polysaccharide content of the product is above 36.4%, and the antioxidant capacity of lonicera japonica polysaccharide is more than 72.3%.

Description

technical field [0001] The invention relates to the technical field of sugar processing technology, in particular to an extraction technology of honeysuckle polysaccharide. Background technique [0002] Honeysuckle has the effect of clearing away heat and detoxifying, cooling and dispersing wind-heat. It has rich pharmacological activities and no side effects, and has been widely used in clinical practice. Honeysuckle is rich in resources and is a traditional Chinese medicine with great development potential. Modern pharmacological experiments and clinical applications have proved that honeysuckle has strong antibacterial effects and good therapeutic effects on various germs. However, there are few research reports on honeysuckle polysaccharides in China at present. Therefore, the research on the preparation technology and immune activity of honeysuckle polysaccharides is conducive to the in-depth understanding of its pharmacological effects and the development of new medic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 邱峰
Owner GUANGXI DONGXING DINGKANG PLASTIC IND
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